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排序方式: 共有104条查询结果,搜索用时 62 毫秒
81.
Guillaume P. Grolez Giorgia Chinig Alexandre Barras Mehdi Hammadi Lucile Noyer Kateryna Kondratska Etmar Bulk Thibauld Oullier Sverine Marionneau-Lambot Marilyne Le Me Stphanie Rtif Stphanie Lerondel Antonino Bongiovanni Tullio Genova Sbastien Roger Rabah Boukherroub Albrecht Schwab Alessandra Fiorio Pla Dimitra Gkika 《International journal of molecular sciences》2022,23(12)
In the fight against prostate cancer (PCa), TRPM8 is one of the most promising clinical targets. Indeed, several studies have highlighted that TRPM8 involvement is key in PCa progression because of its impact on cell proliferation, viability, and migration. However, data from the literature are somewhat contradictory regarding the precise role of TRPM8 in prostatic carcinogenesis and are mostly based on in vitro studies. The purpose of this study was to clarify the role played by TRPM8 in PCa progression. We used a prostate orthotopic xenograft mouse model to show that TRPM8 overexpression dramatically limited tumor growth and metastasis dissemination in vivo. Mechanistically, our in vitro data revealed that TRPM8 inhibited tumor growth by affecting the cell proliferation and clonogenic properties of PCa cells. Moreover, TRPM8 impacted metastatic dissemination mainly by impairing cytoskeleton dynamics and focal adhesion formation through the inhibition of the Cdc42, Rac1, ERK, and FAK pathways. Lastly, we proved the in vivo efficiency of a new tool based on lipid nanocapsules containing WS12 in limiting the TRPM8–positive cells’ dissemination at metastatic sites. Our work strongly supports the protective role of TRPM8 on PCa progression, providing new insights into the potential application of TRPM8 as a therapeutic target in PCa treatment. 相似文献
82.
Dr. Tatjana Ruks Dr. Kateryna Loza Dr. Marc Heggen Prof. Dr. Christian Ottmann Prof. Dr. Peter Bayer Dr. Christine Beuck Prof. Dr. Matthias Epple 《Chembiochem : a European journal of chemical biology》2021,22(8):1456-1463
The surface of ultrasmall gold nanoparticles with an average diameter of 1.55 nm was conjugated with a 14-3-3 protein-binding peptide derived from CRaf. Each particle carries 18 CRaf peptides, leading to an overall stoichiometry of Au(115)Craf(18). The binding to the protein 14-3-3 was probed by isothermal titration calorimetry (ITC) and fluorescence polarization spectroscopy (FP). The dissociation constant (KD) was measured as 5.0 μM by ITC and 0.9 μM by FP, which was close to the affinity of dissolved CRaf to 14-3-3σ. In contrast to dissolved CRaf, which alone did not enter HeLa cells, CRAF-conjugated gold nanoparticles were well taken up by HeLa cells, opening the opportunity to target the protein inside a cell. 相似文献
83.
Dmytro Nozdrenko Olga Abramchuk Svitlana Prylutska Oksana Vygovska Vasil Soroca Kateryna Bogutska Sergii Khrapatyi Yuriy Prylutskyy Peter Scharff Uwe Ritter 《International journal of molecular sciences》2021,22(9)
The widespread use of glyphosate as a herbicide in agriculture can lead to the presence of its residues and metabolites in food for human consumption and thus pose a threat to human health. It has been found that glyphosate reduces energy metabolism in the brain, its amount increases in white muscle fibers. At the same time, the effect of chronic use of glyphosate on the dynamic properties of skeletal muscles remains practically unexplored. The selected biomechanical parameters (the integrated power of muscle contraction, the time of reaching the muscle contraction force its maximum value and the reduction of the force response by 50% and 25% of the initial values during stimulation) of muscle soleus contraction in rats, as well as blood biochemical parameters (the levels of creatinine, creatine phosphokinase, lactate, lactate dehydrogenase, thiobarbituric acid reactive substances, hydrogen peroxide, reduced glutathione and catalase) were analyzed after chronic glyphosate intoxication (oral administration at a dose of 10 μg/kg of animal weight) for 30 days. Water-soluble C60 fullerene, as a poweful antioxidant, was used as a therapeutic nanoagent throughout the entire period of intoxication with the above herbicide (oral administration at doses of 0.5 or 1 mg/kg). The data obtained show that the introduction of C60 fullerene at a dose of 0.5 mg/kg reduces the degree of pathological changes by 40–45%. Increasing the dose of C60 fullerene to 1 mg/kg increases the therapeutic effect by 55–65%, normalizing the studied biomechanical and biochemical parameters. Thus, C60 fullerenes can be effective nanotherapeutics in the treatment of glyphosate-based herbicide poisoning. 相似文献
84.
Andrew J. Allen Igor Levin Russell A. Maier Suzanne E. Witt Fan Zhang Ivan Kuzmenko 《Journal of the American Ceramic Society》2021,104(6):2442-2448
The first in situ characterization of the pore morphology evolution during the cold sintering process (CSP) is presented using small-angle X-ray scattering methods. For practical reasons, measurements have been made on a model system, KH2PO4 (KDP). The scattering signal revealed a striking behavior that could be modeled with nanoscale structural features associated with the dissolution and reprecipitation of KDP close to the grain/pore interface during CSP. The prospects for future more quantitative experiments under a range of temperature and pressure conditions, as well as for studies of more technologically important materials such as ZnO are considered. 相似文献
85.
86.
Hrechaniuk N.I. Khomenko O.V. Smashnyuk Yu. A. Kuzmenko N. N. Kulak L. D. 《Powder Metallurgy and Metal Ceramics》2019,58(5-6):301-306
Powder Metallurgy and Metal Ceramics - The conditions of high-speed vacuum evaporation–condensation for producing granulated relite (a eutectic alloy of tungsten monocarbide and semicarbide)... 相似文献
87.
Kateryna Muzyka Khalku Karim Antonio Guerreiro Alessandro Poma Sergey Piletsky 《Nanoscale research letters》2014,9(1):154
A novel optimized protocol for solid-state synthesis of molecularly imprinted polymer nanoparticles (nanoMIPs) with specificity for antibiotic vancomycin is described. The experimental objective was optimization of the synthesis parameters (factors) affecting the yield of obtained nanoparticles which have been synthesized using the first prototype of an automated solid-phase synthesizer. Applications of experimental design (or design of experiments) in optimization of nanoMIP yield were carried out using MODDE 9.0 software. The factors chosen in the model were the amount of functional monomers in the polymerization mixture, irradiation time, temperature during polymerization, and elution temperature. In general, it could be concluded that the irradiation time is the most important and the temperature was the least important factor which influences the yield of nanoparticles. Overall, the response surface methodology proved to be an effective tool in reducing time required for optimization of complex experimental conditions. 相似文献
88.
Hybrid inks are often used for the advertising products because a variety of effects can serve as an added value. Much attention is paid to the inks application settings, using the hybrid inks at the hi-tech offset equipment and color features of images. The thorough research of the different printing settings with the use of modern inks and efficient equipment, is an urgent problem. Work reveals some new facts about characteristics for printability such as emulsification and influences of ink's additives on color characteristics of imprints. The experimental inks were developed to define the technological settings of offset printing. The result of research showed that conventional inks have the considerably high degree of emulsification comparing to experimental hybrid inks. Also it was discovered that the UV components influence the emulsification. An optimal water-ink balance and stable printing process can be achieved with adding of 2%-10% of the UV-component to the hybrid inks. Inks application and drying settings as well as color features of the imprints are analyzed in the context of offset printing. 相似文献
89.
Ultrastructural study of mouse adipose‐derived stromal cells induced towards osteogenic direction
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Oleg Tsupykov Alina Ustymenko Vitaliy Kyryk Ekaterina Smozhanik Kateryna Yatsenko Gennadii Butenko Galina Skibo 《Microscopy research and technique》2016,79(6):557-564
We investigated the ultrastructural characteristics of mouse adipose‐derived stem/stromal cells (ASCs) induced towards osteogenic lineage. ASCs were isolated from adipose tissue of FVB‐Cg‐Tg(GFPU)5Nagy/J mice and expanded in monolayer culture. Flow cytometry, histochemical staining, and electron microscopy techniques were used to characterize the ASCs with respect to their ability for osteogenic differentiation capacity. Immunophenotypically, ASCs were characterized by high expression of the CD44 and CD90 markers, while the relative content of cells expressing CD45, CD34 and CD117 markers was <2%. In assays of differentiation, the positive response to osteogenic differentiation factors was observed and characterized by deposition of calcium in the extracellular matrix and alkaline phosphatase production. Electron microscopy analysis revealed that undifferentiated ASCs had a rough endoplasmic reticulum with dilated cisterns and elongated mitochondria. At the end of the osteogenic differentiation, the ASCs transformed from their original fibroblast‐like appearance to having a polygonal osteoblast‐like morphology. Ultrastructurally, these cells were characterized by large euchromatic nucleus and numerous cytoplasm containing elongated mitochondria, a very prominent rough endoplasmic reticulum, Golgi apparatus and intermediate filament bundles. Extracellular matrix vesicles of variable size similar to the calcification nodules were observed among collagen fibrils. Our data provide the ultrastructural basis for further studies on the cellular mechanisms involved in osteogenic differentiation of mouse adipose‐derived stem/stromal cells. Microsc. Res. Tech. 79:557–564, 2016. © 2016 Wiley Periodicals, Inc. 相似文献
90.
Elizabeth Arkhangelsky Denis Kuzmenko Norm V. Gitis Michael Vinogradov Suresh Kuiry Vitaly Gitis 《Tribology Letters》2007,28(2):109-116
Polymer membranes are often used in water treatment industry, as a reliable economic solution for purification of surface
water streams. Among a variety of membrane processes the ultrafiltration (UF) membranes combine high pathogen removal ability
with minimal energy consumption. Since the membranes are considered to be chemically and mechanically stable, in-place cleaning
is routinely performed with strong oxidizing agents, such as hypochlorite. The results of the current study, however, clearly
indicate that the mechanical strength of UF membranes deteriorates upon hypochlorite cleaning. Values of ultimate tensile
strength, ultimate elongation and Young’s modulus decreased as the dose (concentration × contact time) of hypochlorite was
increased, with the decreases being more marked for cellulose acetate membranes than for polyethersulfone membranes. Scanning
probe microscopy showed membrane aging in response to hypochlorite treatment. The aging was related to gradual chain breaking
in membrane skin layer, as reflected by the disappearance of O–C–O and C–S absorbance peaks in ATR-FTIR spectra of treated
cellulose acetate and polyethersulfone membranes, respectively. Since the degree of scission was linearly related to the deterioration
in ultimate tensile strength, we here propose that ultimate tensile strength measurements can be used as a simple macroscopic
test for periodic inspection of the degree of degradation of UF membranes exposed to in-place chemical cleaning. 相似文献