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181.
Michaela Oeller Sandra Laner-Plamberger Linda Krisch Eva Rohde Dirk Strunk Katharina Schallmoser 《International journal of molecular sciences》2021,22(10)
Numerous cell-based therapeutics are currently being tested in clinical trials. Human platelet lysate (HPL) is a valuable alternative to fetal bovine serum as a cell culture medium supplement for a variety of different cell types. HPL as a raw material permits animal serum-free cell propagation with highly efficient stimulation of cell proliferation, enabling humanized manufacturing of cell therapeutics within a reasonable timeframe. Providers of HPL have to consider dedicated quality issues regarding identity, purity, potency, traceability and safety. Release criteria have to be defined, characterizing the suitability of HPL batches for the support of a specific cell culture. Fresh or expired platelet concentrates from healthy blood donors are the starting material for HPL preparation, according to regulatory requirements. Pooling of individual platelet lysate units into one HPL batch can balance donor variation with regard to essential platelet-derived growth factors and cytokines. The increasingly applied pathogen reduction technologies will further increase HPL safety. In this review article, aspects and regulatory requirements of whole blood donation and details of human platelet lysate manufacturing are presented. International guidelines for raw materials are discussed, and defined quality controls, as well as release criteria for safe and GMP-compliant HPL production, are summarized. 相似文献
182.
Scientometrics - Identification of important works and assessment of importance of publications in vast scientific corpora are challenging yet common tasks subjected by many research projects.... 相似文献
183.
Illustrates, in a case study of a 42-yr-old woman, known factors that facilitate mirroring (e.g., therapeutic blind spots, unconscious identifications, temporary regressions in therapist or supervisor) and describes the previously unrecognized impact of the therapeutic milieu on the occurrence of mirroring. Consideration of the therapeutic milieu allows the identification of situations in which mirroring is most likely to occur and those in which mirroring can be falsely suspected. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
184.
Youngrok Kim Katharina Broch Woocheol Lee Heebeom Ahn Jonghoon Lee Daekyoung Yoo Junwoo Kim Seungjun Chung Henning Sirringhaus Keehoon Kang Takhee Lee 《Advanced functional materials》2020,30(28)
In organic device applications, a high contact resistance between metal electrodes and organic semiconductors prevents an efficient charge injection and extraction, which fundamentally limits the device performance. Recently, various contact doping methods have been reported as an effective way to resolve the contact resistance problem. However, the contact doping has not been explored extensively in organic field effect transistors (OFETs) due to dopant diffusion problem, which significantly degrades the device stability by damaging the ON/OFF switching performance. Here, the stability of a contact doping method is improved by incorporating “dopant‐blockade molecules” in the poly(2,5‐bis(3‐hexadecylthiophen‐2‐yl)thieno[3,2‐b]thiophene) (PBTTT) film in order to suppress the diffusion of the dopant molecules. By carefully selecting the dopant‐blockade molecules for effectively blocking the dopant diffusion paths, the ON/OFF ratio of PBTTT OFETs can be maintained over 2 months. This work will maximize the potential of OFETs by employing the contact doping method as a promising route toward resolving the contact resistance problem. 相似文献
185.
Dominic J. Wales Qun Cao Katharina Kastner Erno Karjalainen Graham N. Newton Victor Sans 《Advanced materials (Deerfield Beach, Fla.)》2018,30(26)
The formulation of advanced molecular materials with bespoke polymeric ionic‐liquid matrices that stabilize and solubilize hybrid organic–inorganic polyoxometalates and allow their processing by additive manufacturing, is effectively demonstrated. The unique photo and redox properties of nanostructured polyoxometalates are translated across the scales (from molecular design to functional materials) to yield macroscopic functional devices with reversible photochromism. These properties open a range of potential applications including reversible information storage based on controlled topological and temporal reduction/oxidation of pre‐formed printed devices. This approach pushes the boundaries of 3D printing to the molecular limits, allowing the freedom of design enabled by 3D printing to be coupled with the molecular tuneability of polymerizable ionic liquids and the photoactivity and orbital engineering possible with hybrid polyoxometalates. 相似文献
186.
187.
Surface Features of Recombinant Spider Silk Protein eADF4(κ16)‐Made Materials are Well‐Suited for Cardiac Tissue Engineering
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Jana Petzold Tamara B. Aigner Filip Touska Katharina Zimmermann Thomas Scheibel Felix B. Engel 《Advanced functional materials》2017,27(36)
Cardiovascular diseases causing high morbidity and mortality represent a major socioeconomic burden. The primary cause of impaired heart function is often the loss of cardiomyocytes. Thus, novel therapies aim at restoring the lost myocardial tissue. One promising approach is cardiac tissue engineering. Previously, it is shown that Antheraea mylitta silk protein fibroin is a suitable material for cardiac tissue engineering, however, its quality is difficult to control. To overcome this limitation, the interaction of primary rat heart cells with engineered Araneus diadematus fibroin 4 (κ16) (eADF4(κ16)) is investigated here, which is engineered based on the sequence of ADF4 by replacing the glutamic acid residue in the repetitive unit of its core domain with lysine. The data demonstrate that cardiomyocytes, fibroblasts, endothelial cells, and smooth muscle cells attach well to eADF4(κ16) films on glass coverslips which provide an engineered surface with a polycationic character. Moreover, eADF4(κ16) films have, in contrast to fibronectin films, no hypertrophic effect but allow the induction of cardiomyocyte hypertrophy. Finally, cardiomyocytes grown on eADF4(κ16) films respond to pro‐proliferative factors and exhibit proper cell‐to‐cell communication and electric coupling. Collectively, these data demonstrate that designed recombinant eADF4(κ16)‐based materials are promising materials for cardiac tissue engineering. 相似文献
188.
Spectroscopic Indications of Tunnel Barrier Charging as the Switching Mechanism in Memristive Devices
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Benedikt Arndt Francesco Borgatti Francesco Offi Monifa Phillips Pedro Parreira Thorsten Meiners Stephan Menzel Katharina Skaja Giancarlo Panaccione Donald A. MacLaren Rainer Waser Regina Dittmann 《Advanced functional materials》2017,27(45)
Resistive random access memory is a promising, energy‐efficient, low‐power “storage class memory” technology that has the potential to replace both flash storage and on‐chip dynamic memory. While the most widely employed systems exhibit filamentary resistive switching, interface‐type switching systems based on a tunable tunnel barrier are of increasing interest. They suffer less from the variability induced by the stochastic filament formation process and the choice of the tunnel barrier thickness offers the possibility to adapt the memory device current to the given circuit requirements. Heterostructures consisting of a yttria‐stabilized zirconia (YSZ) tunnel barrier and a praseodymium calcium manganite (PCMO) layer are employed. Instead of spatially localized filaments, the resistive switching process occurs underneath the whole electrode. By employing a combination of electrical measurements, in operando hard X‐ray photoelectron spectroscopy and electron energy loss spectroscopy, it is revealed that an exchange of oxygen ions between PCMO and YSZ causes an electrostatic modulation of the effective height of the YSZ tunnel barrier and is thereby the underlying mechanism for resistive switching in these devices. 相似文献
189.
Katharina Brandt Stefan Dötterl Wittko Francke Manfred Ayasse Paulo Milet-Pinheiro 《Journal of chemical ecology》2017,43(1):4-12
The pollen diet provided by adult bees to their offspring varies immensely. While some species collect pollen on several plants irrespective of their phylogenetic relatedness (polyleges), others collect only on plants within a genus or family (oligoleges). Floral scents play a central role in bee-plant interactions. To locate flowers, polyleges are assumed to rely on compounds commonly found as floral scent constituents, whereas oligoleges rely on unusual compounds to recognize host flowers unambiguously. Campanula flowers are visited by both polylectic and oligolectic species, and their scent bouquets consist of common and unusual (e.g., spiroacetals) volatiles. In a comparative approach, we performed electroantennographic analyses to investigate the antennal responses of three polyleges and three oligoleges to three common volatiles and four spiroacetals. We hypothesized that: 1) oligoleges and polyleges should respond similarly to common flower volatiles, and 2) Campanula oligoleges should be more sensitive to spiroacetals than are polyleges. In corroboration, we found that antennal sensitivity to common volatiles was similar among bees irrespective of pollen diet, whereas oligoleges of Campanula were more sensitive to spiroacetals than polyleges. Newly emerged bees of the Campanula oligolege Chelostoma rapunculi rely on spiroacetals for recognizing host-flowers, and our results suggest that this might also be true for other Campanula oligoleges, since Chelostoma campanularum and Hoplitis mitis also were able to perceive these specific volatiles at very low concentrations. Together, our results provide interesting insights into the significance of olfactory adaptations in oligolectic and polylectic bee species. 相似文献
190.
The 5′‐AG5CC‐3′ Fragment from the Human CPEB3 Ribozyme Forms an Ultrastable Parallel RNA G‐Quadruplex
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Katharina Kulikov Dr. Senada Nozinovic Dr. Stephanie Kath‐Schorr 《Chembiochem : a European journal of chemical biology》2017,18(11):969-973
An unusually thermostable G‐quadruplex is formed by a sequence fragment of a naturally occurring ribozyme, the human CPEB3 ribozyme. Strong evidence is provided for the formation of a uniquely stable intermolecular G‐quadruplex structure consisting of five tetrad layers, by using CD spectroscopy, UV melting curves, 2D NMR spectroscopy, and gel shift analysis. The cationic porphyrin TMPyP4 destabilizes the complex. 相似文献