Alpha1-adrenergic receptors in human spinal cord: specific localized expression of mRNA encoding alpha1-adrenergic receptor subtypes at four distinct levels

alpha1-Adrenergic receptors (alpha1ARs) are important in lower urinary tract syndromes such as benign prostatic hypertrophy and bladder irritability. Spinal cord alpha1ARs have been postulated to play a role in modulating these diseases, yet alpha1AR subtype (alpha1a, alpha1b, alpha1d) neuronal localization in human spinal cord has not been described. We therefore tested the hypothesis that alpha1AR subtype distribution varies according to specific spinal cord tract and level. In situ hybridization was performed to identify cell bodies containing alpha1AR subtype mRNA at four levels of human spinal cord (cervical enlargement, thoracic, lumbar, sacral). alpha1AR mRNA is present in ventral gray matter only (ventral>dorsal; sacral>lumbar=thoracic>cervical). Signaling cell bodies were detected in anterior horn motor neurons at all levels; dorsal nucleus of Clarke and intermediolateral columns in cervical enlargement, thoracic and lumbar spinal cord regions; and parasympathetic nucleus in sacral spinal cord. Although all three alpha1AR subtypes are present throughout human spinal cord, alpha1d mRNA predominates overall. If confirmed at a protein level, these findings may contribute to the development of new therapeutic strategies in the treatment of several human diseases.     

Performance improvement integration: a whole systems approach

Performance improvement integration in health care organizations is a challenge for health care leaders. Required for accreditation by the Joint Commission on Accreditation of Healthcare Organizations (Joint Commission), performance improvement (PI) can be designed as a sustainable model for performance to survive in a turbulent period. Central Baptist Hospital developed a model for PI that focused on strategy established by the leadership team, delineated responsibility through the organizational structure of shared governance, and accountability for outcomes evidenced through the organization's profitability. Such an approach integrated into the culture of the organization can produce positive financial margins, positive customer satisfaction, and commendations from the Joint Commission.     

Urinary monitoring of saccharin and acesulfame-K as biomarkers of exposure to these additives

A method was developed to determine the levels of two intense sweeteners, saccharin and acesulfame-K, in human urine. Measurement of these two analytes in urine allowed an assessment to be made of dietary exposure to the sweeteners using intake/excretion curves. This paper describes an intake/excretion study in which 22 volunteers consumed known amounts of saccharin and acesulfame-K at five different levels ranging between 1.4 and 70mg of saccharin (0.7- 30% of the ADI for a 60kg person) and 2.2-102mg of acesulfame-K (0.4-19% of the ADI for a 60kg person). Urine collections were then carried out by the volunteers for 24 h after each dose. The data obtained from this study were used to construct intake/excretion curves for both sweeteners. To test the methodology developed, 188 volunteers aged between 3 and 74 years were asked to carry out a 24-h urine collection whilst keeping a 48-h food diary. Comparison of the intake data obtained using the biomarker approach with the questionnaire-based results was generally good, although discrepancies due to the format and assessment of the questionnaire data were noticed.     

Design and characterization of a horizontal thermal gradient cloud condensation nucleus spectrometer

We report the design and characterization of a continuous-flow horizontal thermal gradient cloud condensation nucleus spectrometer (CCNS). The calibration of supersaturation inside the CCNS chamber using monodisperse NaCl aerosols shows that it is important to experimentally determine the supersaturation profile of the instrument, rather than relying on theoretical calculations based on measurements of the temperature gradient. The latter method significantly overestimates the actual supersaturation, mainly because of the discrepancy between measured and actual temperature gradients and the non-ideality in droplet samplings. Laboratory experiments were also performed to validate the instrumental performance and to compare the cloud condensation nuclei (CCN) activation results with theoretical predictions based on Köhler theory. In the current configuration, the operational range of the CCNS has been verified to be between 0.08% and 0.9% supersaturation, with potential for further range enhancement. Using a computer-controlled motorized sampling system, we have demonstrated that CCN activation experiments can be routinely performed with much higher time resolution, suggesting excellent potential of this CCNS instrument for airborne measurements.     

Randomised restarted search in ILP

Recent statistical performance studies of search algorithms in difficult combinatorial problems have demonstrated the benefits of randomising and restarting the search procedure. Specifically, it has been found that if the search cost distribution of the non-restarted randomised search exhibits a slower-than-exponential decay (that is, a “heavy tail”), restarts can reduce the search cost expectation. We report on an empirical study of randomised restarted search in ILP. Our experiments conducted on a high-performance distributed computing platform provide an extensive statistical performance sample of five search algorithms operating on two principally different classes of ILP problems, one represented by an artificially generated graph problem and the other by three traditional classification benchmarks (mutagenicity, carcinogenicity, finite element mesh design). The sample allows us to (1) estimate the conditional expected value of the search cost (measured by the total number of clauses explored) given the minimum clause score required and a “cutoff” value (the number of clauses examined before the search is restarted), (2) estimate the conditional expected clause score given the cutoff value and the invested search cost, and (3) compare the performance of randomised restarted search strategies to a deterministic non-restarted search. Our findings indicate striking similarities across the five search algorithms and the four domains, in terms of the basic trends of both the statistics (1) and (2). Also, we observe that the cutoff value is critical for the performance of the search algorithm, and using its optimal value in a randomised restarted search may decrease the mean search cost (by several orders of magnitude) or increase the mean achieved score significantly with respect to that obtained with a deterministic non-restarted search. Editors: Rui Camacho     

Transient inactivation of the ventral tegmental area selectively disrupts the expression of conditioned place preference for pup- but not cocaine-paired contexts.

The ventral tegmental area (VTA) plays a critical role in motivated behavior. However, it remains unclear whether intact VTA function is necessary for motivated behavior to seek contexts repeatedly paired with natural stimuli and/or pharmacological stimuli. In the present study, conditioned place preference (CPP) was induced with highly salient natural or drug stimuli attributed with strong incentive–motivational value in each of 2 female models: Postpartum females were conditioned to associate one unique context in the CPP apparatus with young offspring (pups) and a second context with a neutral stimulus, and virgin females were conditioned to associate unique contexts with cocaine (5 mg/kg ip) and saline injections. Immediately before CPP testing, each female received a microinfusion of bupivacaine bilaterally into the VTA to transiently inactivate the region; subjects were also tested after saline microinfusion into the VTA. Postpartum females’ preference for the pup-paired context was abolished by VTA inactivation but was restored to high control levels after saline microinfusion. In separate tests, VTA inactivation also reduced motivated pup licking and pup retrieval in postpartum females, suggesting that intact VTA function is required for the expression of both pup CPP and motivated pup-directed behaviors. Cocaine CPP remained unaffected by VTA inactivation. Locomotion was not affected by VTA microinfusions but was severely impaired by bupivacaine microinfusions into the substantia nigra. We concluded that the VTA is differentially involved in the expression of conditioned preference for contexts paired with pups, a salient natural stimulus, and contexts paired with cocaine. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A 100% renewable electricity generation system for New Zealand utilising hydro,wind, geothermal and biomass resources

The New Zealand electricity generation system is dominated by hydro generation at approximately 60% of installed capacity between 2005 and 2007, augmented with approximately 32% fossil-fuelled generation, plus minor contributions from geothermal, wind and biomass resources. In order to explore the potential for a 100% renewable electricity generation system with substantially increased levels of wind penetration, fossil-fuelled electricity production was removed from an historic 3-year data set, and replaced by modelled electricity production from wind, geothermal and additional peaking options. Generation mixes comprising 53–60% hydro, 22–25% wind, 12–14% geothermal, 1% biomass and 0–12% additional peaking generation were found to be feasible on an energy and power basis, whilst maintaining net hydro storage. Wind capacity credits ranged from 47% to 105% depending upon the incorporation of demand management, and the manner of operation of the hydro system. Wind spillage was minimised, however, a degree of residual spillage was considered to be an inevitable part of incorporating non-dispatchable generation into a stand-alone grid system. Load shifting was shown to have considerable advantages over installation of new peaking plant. Application of the approach applied in this research to countries with different energy resource mixes is discussed, and options for further research are outlined.     

Towards trapped antihydrogen

Substantial progress has been made in the last few years in the nascent field of antihydrogen physics. The next big step forward is expected to be the trapping of the formed antihydrogen atoms using a magnetic multipole trap. ALPHA is a new international project that started to take data in 2006 at CERN’s Antiproton Decelerator facility. The primary goal of ALPHA is stable trapping of cold antihydrogen atoms to facilitate measurements of its properties. We discuss the status of the ALPHA project and the prospects for antihydrogen trapping.     

Controlled Sub‐Micrometer Hierarchical Textures Engineered in Polymeric Fibers and Microchannels via Thermal Drawing

The controlled texturing of surfaces at the micro‐ and nanoscales is a powerful method for tailoring how materials interact with liquids, electromagnetic waves, or biological tissues. The increasing scientific and technological interest in advanced fibers and fabrics has triggered a strong motivation for leveraging the use of textures on fiber surfaces. Thus far however, fiber‐processing techniques have exhibited an inherent limitation due to the smoothing out of surface textures by polymer reflow, restricting achievable feature sizes. In this article, a theoretical framework is established from which a strategy is developed to reduce the surface tension of the textured polymer, thus drastically slowing down thermal reflow. With this approach the fabrication of potentially kilometers‐long polymer fibers with controlled hierarchical surface textures of unprecedented complexity and with feature sizes down to a few hundreds of nanometers is demonstrated, two orders of magnitude below current configurations. Using such fibers as molds, 3D microchannels are also fabricated with textured inner surfaces within soft polymers such as poly(dimethylsiloxane), at dimensions and a degree of simplicity impossible to reach with current techniques. This strategy for the texturing of high curvature surfaces opens novel opportunities in bioengineering, regenerative scaffolds, microfluidics, and smart textiles.     

Superelastic Multimaterial Electronic and Photonic Fibers and Devices via Thermal Drawing

Electronic and photonic fiber devices that can sustain large elastic deformation are becoming key components in a variety of fields ranging from healthcare to robotics and wearable devices. The fabrication of highly elastic and functional fibers remains however challenging, which is limiting their technological developments. Simple and scalable fiber‐processing techniques to continuously codraw different materials within a polymeric structure constitute an ideal platform to realize functional fibers and devices. Despite decades of research however, elastomeric materials with the proper rheological attributes for multimaterial fiber processing cannot be identified. Here, the thermal drawing of hundreds‐of‐meters long multimaterial optical and electronic fibers and devices that can sustain up to 500% elastic deformation is demonstrated. From a rheological and microstructure analysis, thermoplastic elastomers that can be thermally drawn at high viscosities (above 10³ Pa s), allowing the encapsulation of a variety of microstructured, soft, and rigid materials are identified. Using this scalable approach, fiber devices combining high performance, extreme elasticity, and unprecedented functionalities, allowing novel applications in smart textiles, robotics, or medical implants, are demonstrated.