Differential hormonal regulation of estrogen receptors ERalpha and ERbeta and androgen receptor expression in rat efferent ductules

Estrogen receptors, in addition to the androgen receptor (AR), are expressed at high levels in efferent ductules of the male reproductive tract and it is now well recognized that estrogen receptor (ER) alpha is required for the maintenance of normal structure and function of the ductules. However, little is known regarding the hormonal regulation of the receptors themselves in the male. In the present study, efferent ductule ligation and castration, followed by replacement with testosterone, dihydro-testosterone (DHT) or estradiol was used to investigate the relative importance of circulating and luminal sources of steroid for the modulation of ERalpha, ERbeta and AR in rat efferent ductules. Uni- or bilateral castration and ligation did not affect the expression of ERalpha and ERbeta, but bilateral castration caused down-regulation of AR. Replacement with DHT and testosterone alone or in combination with estradiol caused the recovery of AR expression to control levels. A slight recovery of AR was also observed after estrogen replacement. ERalpha expression was decreased to nearly undetectable levels after estrogen replacement. On the other hand, ERbeta did not show evident effects following any of the treatments, suggesting a constitutive expression of this receptor. This differential modulation of the steroid hormone receptors highlights the importance of maintaining a physiological androgen-estrogen balance to regulate the structure and function of efferent ductules in the male.     

Characterizing future large,rapid changes in aggregated wind power using Numerical Weather Prediction spatial fields

A critical limiting factor to the successful deployment of a large proportion of wind power in power systems is its predictability. Power system operators play a vital role in maintaining system security, and this task is greatly aided by useful characterizations of future system operations. A wind farm power forecast generally relies on the forecast output from a Numerical Weather Prediction (NWP) model, typically at a single grid point in the model to represent the wind farm's physical location. A key limitation of this approach is the spatial misplacement of weather features often found in NWP forecasts. This paper presents a methodology to display wind forecast information from multiple grid points at hub height around the wind farm location. If the raw forecast wind speeds at hub height at multiple grid points were to be displayed directly, they would be misleading as the NWP outputs take account of the estimated local surface roughness and terrain at each grid point. Hence, the methodology includes a transformation of the wind speed at each grid point to an equivalent value that represents the surface roughness and terrain at the chosen single grid point for the wind farm site. The chosen‐grid‐point‐equivalent wind speeds for the wind farm can then be transformed to available wind farm power. The result is a visually‐based decision support tool which can help the forecast user to assess the possibilities of large, rapid changes in available wind power from wind farms. A number of methods for displaying the field for multiple wind farms are discussed. The chosen‐grid‐point‐equivalent transformation also has other potential applications in wind power forecasting such as assessing deterministic forecast uncertainty and improving downscaling results. Copyright © 2008 John Wiley & Sons, Ltd.     

Ultrasound velocity and attenuation of porcine soft tissues with respect to structure and composition: II. Skin and backfat

Ultrasound is regarded as a promising method to determine the intramuscular fat content of pork loin. At intact carcasses, the signal passes the backfat whose ultrasound parameters (sound velocity and attenuation) have not been fully investigated. This study intended to collect a dataset of ultrasound parameters for individual backfat layers and to elucidate relationships with structural and compositional characteristics. In-vitro measurements at 10 MHz were conducted on backfat samples of pork carcasses representative for German populations. The average sound velocity ranged from 1436 ± 9 to 1470 ± 37 ms(-1) for the fat layers, and 1682 ± 23 ms(-1) for skin. Velocity of the compound backfat decreased with overall thickness. Attenuation was not affected by thickness ranging between 1.6 ± 0.7 and 2.7 ± 1.5 dB MHz(-1)cm(-1) for all layers. Sound velocity was negatively correlated with fat content and dry matter. The obtained results are anticipated to improve signal correction prior to spectral analysis of ultrasound measurements at intact carcasses.     

Analysis of incurred crystal violet in Atlantic salmon (Salmo salar L.): comparison between the analysis of crystal violet as an individual parent and leucocrystal violet and as total crystal violet after oxidation with 2,3-dichloro-5,6-dicyanobenzoquinone

Due to on-going concern about the occurrence of triphenylmethane dye residues in fish destined for human consumption, a depletion study of crystal violet in salmon was carried out. Atlantic salmon less than 12 months old were exposed to crystal violet in fresh water at 15°C and subsequently sampled at 1, 7, 14, 28, 63 and 91 days after exposure. The salmon were then analysed by two analytical methods. In the first method, 2,3-dichloro-5,6-dicyanobenzoquinone (DDQ) was used to oxidise leucocrystal violet to its parent form. Total parent crystal violet was then analysed by LC-MS/MS. In the second method, crystal violet and leucocrystal violet were analysed individually by LC-MS/MS without oxidation. Both methods gave comparable results for total crystal violet concentrations, with a correlation of r(2)=0.69. Statistical treatment for 88 incurred salmon samples showed no significant difference between the two sets of results with t=1.68 and t(crit)=1.99. Up to 98% of crystal violet was metabolised to its leuco form in the salmon after 1 day of exposure and could be detected at significant concentrations (approximately 20 μg kg(-1)) 91 days after exposure. The depletion data also suggest that crystal violet has a half-life of approximately 15-16 days in salmon.     

Enhanced Solubility and Ecological Impact of Atmospheric Phosphorus Deposition upon Extended Seawater Exposure

Atmospheric P solubility affects the amount of P available for phytoplankton in the surface ocean, yet our understanding of the timing and extent of atmospheric P solubility is based on short-term leaching experiments where conditions may differ substantially from the surface ocean. We conducted longer- term dissolution experiments of atmospheric aerosols in filtered seawater, and found up to 9-fold greater dissolution of P after 72 h compared to instantaneous leaching. Samples rich in anthropogenic materials released dissolved inorganic P (DIP) faster than mineral dust. To gauge the effect of biota on the fate of atmospheric P, we conducted field incubations with aerosol samples collected in the Sargasso Sea and Red Sea. In the Sargasso Sea phytoplankton were not P limited, and biological activity enhanced DIP release from aerosols, and aerosols induced biological mineralization of dissolved organic P in seawater, leading to DIP accumulation. However, in the Red Sea where phytoplankton were colimited by P and N, soluble P was rapidly consumed by phytoplankton following aerosol enrichment. Our results suggest that atmospheric P dissolution could continue over multiple days once reaching the surface ocean, and that previous estimates of atmospheric P deposition may underestimate the contribution from this source.     

Ability of patients to recall habitual contact lens products and enhancement of recall using photographic aids

Purpose

To determine the proportion of soft contact lens (CL) wearers who are able to recall their habitual products (lenses and care system) correctly from memory, and to evaluate the value of using photographic aids (PAs) to improve recall.

Methods

103 soft lens wearers attended 2 visits to investigate their habitual CL product use. At the first visit they were asked to recall which products they were using and then to identify their products from PAs. They returned for a second visit with their products for confirmation.

Results

51% correctly reported their lens brands from memory alone, which improved to 87% with the use of the PAs (p < 0.001). 41% correctly reported their habitual care system from memory alone, which improved to 80% with the use of PAs (p < 0.001). Females were better at recalling care system brand names than males (49% versus 27% correct, p = 0.040) and wearers with more than 1 year experience with their habitual CLs had better recall than those with up to 1 year experience (63% versus 27%, p = 0.014).

Conclusion

Less than 50% of contact lens wearers were able to recall the names of their habitual lens and lens care products correctly from memory. PAs improved this recall significantly for both contact lenses and contact lens care systems.     

Revised fish aging techniques improve fish contaminant trend analyses in the face of changing Great Lakes food webs

Incorporation of fish age into the assessment of status and trends for persistent, bioaccumulative and toxic chemicals in the Great Lakes has become an important step for the U.S. EPA's Great Lakes Fish Monitoring and Surveillance Program (GLFMSP). A slowing in the rate of decline for total PCBs in Lake Huron beginning in 2000, led the Program to complete a retrospective analysis to assess how chemical contamination may be influenced by fish age. Analytical results suggest that fish age is an important variable when assessing contaminant trends and that the Program needed to revise its compositing scheme to group fish according to age, rather than by length, prior to homogenization and chemical analysis. An Interlaboratory comparison study of multiple age structures was performed to identify the most appropriate age estimation structure for the Program. The lake trout (Salvelinus namaycush) maxillae was selected, over the otolith, as the most precise, accurate, and rapidly assessed structure for the Program when compared between laboratories and against the known age from the coded wire tag (CWT). Age-normalization practices can now be implemented when assessing contaminant concentrations and trends for the GLFMSP.     

Estimation of diffuse and point source microbial pollution in the ribble catchment discharging to bathing waters in the north west of England.

Achieving compliance with the mandatory standards of the 1976 Bathing Water Directive (76/160/EEC) is required at all U.K. identified bathing waters. In recent years, the Fylde coast has been an area of significant investments in 'point source' control, which have not proven, in isolation, to satisfactorily achieve compliance with the mandatory, let alone the guide, levels of water quality in the Directive. The potential impact of riverine sources of pollution was first confirmed after a study in 1997. The completion of sewerage system enhancements offered the potential for the study of faecal indicator delivery from upstream sources comprising both point sources and diffuse agricultural sources. A research project to define these elements commenced in 2001. Initially, a desk study reported here, estimated the principal infrastructure contributions within the Ribble catchment. A second phase of this investigation has involved acquisition of empirical water quality and hydrological data from the catchment during the 2002 bathing season. These data have been used further to calibrate the 'budgets' and 'delivery' modelling and these data are still being analysed. This paper reports the initial desk study approach to faecal indicator budget estimation using available data from the sewerage infrastructure and catchment sources of faecal indicators.     

Money Management After Acquired Brain Dysfunction: The Validity of Neuropsychological Assessment.

Objective: To study the money management ability of people with acquired brain dysfunction (ABD) and its relationship to neuropsychological test performance. Subjects or Other Participants: 35 people with ABD and 15 matched controls without ABD. Main Outcome Measures: Case managers completed an experimenter-designed Money Management Survey (MMS), a staff-rating measure of the client's money management abilities. Results: In addition to the problems identified by controls, the group with ABD had other difficulties such as not leaving money aside for essentials and using automatic teller machines. Neuropsychological assessment demonstrated a modest ability to predict the overall MMS, however, the prediction of specific behaviors (problematic impulsive spending and paying the bills or rent late) was more successful. Conclusion: These results provide support for the use of the MMS and indicate that people with ABD have greater problems with specific aspects of money management than do controls. (PsycINFO Database Record (c) 2010 APA, all rights reserved)