Characterization of the lactic acid bacteria in ewe's milk and cheese from northwest Argentina

Indigenous lactic acid bacteria in ewe's milk and artisanal cheese were studied in four samples of fresh raw milk and four 1-month-old cheeses from the provinces of northwest Argentina. Mean growth counts on M17, MRS, and MSE agar media did not show significant differences (P < 0.05) in raw milk and cheeses. Isolates of lactic acid bacteria from milk were identified as Enterococcus (48%), lactococci (14%), leuconostocs (8%), and lactobacilli (30%). All lactococci were identified as Lactococcus lactis (subsp. lactis and subsp. cremoris). Lactobacilli were identified as Lactobacillus plantarum (92%) and Lactobacillus acidophilus (8%). Enterococci (59%) and lactobacilli (41%) were isolated from cheeses. L. plantarum (93%), L. acidophilus (5%), and Lactobacillus casei (2%) were most frequently isolated. L. lactis subsp. lactis biovar diacetylactis strains were considered as fast acid producers. L. lactis subsp. cremoris strains were slow acid producers. L. plantarum and L. casei strains identified from the cheeses showed slow acid production. The majority of the lactobacilli and Lactococcus lactis strains utilized citrate and produced diacetyl and acetoin in milk. Enzyme activities (API-ZYM tests) of lactococci were low, but activities of L. plantarum strains were considerably higher. The predominance of L. plantarum in artisanal cheese is probably important in the ripening of these cheeses due to their physiological and biochemical characteristics.     

A comparison of estimators of output multipliers from incomplete input-output data

This paper presents derivations and empirical tests of three formulas for estimating output multipliers with only limited input-output type data. The first formula assumes only knowledge of the proportion of the value of output of the firm or industry in question which is respent within the region, as well as an estimate of the average of this value over all industries. The second formula assumes this value as well as the relative values of the I-O row totals. The third equation assumes data equivalent to a column in the I-O matrix is known for the specific firm or industry in question. The data requirements for all three formulas are modest but are more for (2) than for (1) and are more for (3) than for (2). All three formulas are shown to be quite reliable but the third gives especially close estimates of the actual multipliers for the several models tested. It is concluded that fairly rough estimates of the average interindustry proportion can be safely used but it is important to obtain the firm or industry expenditure (direct effects) data as accurately as possible.     

Photoaction, temperature and O2 depletion effects in fullerene photoelectrochemical solar cells

It is demonstrated that higher temperature and C₆₀ oxygen depletion increase the photocurrent of fullerene photoelectrochemical solar cells (PEC). Fullerene/iodide electrolyte PEC consisting of intrinsic single crystal C₆₀ in either aqueous 3 M KI, 0.01 M I₂, or 0.1 M tetrabutyl ammonium iodide, 0.3 M LiClO₄ in acetonitrile solution, drive regenerative photoinduced iodide oxidation. The photocurrent is increased by an order of magnitude (to 6.4 μA/cm² under 100 mW/cm² illumination) by an increase of the aqueous cell temperature from 24°C to 82°C. A similar order of magnitude increase in photocurrent is accomplished by O₂ depletion pretreatment (24 h at 400°C in Ar) of the C₆₀ to improve conductivity. However, this latter treatment also irreversibly increases the cell dark current. The spectral action of single crystal C₆₀ is also probed, through the generated photoelectrochemical current in iodide, ferricyanide and sulfuric electrolytes as a function of wavelength. Band edges are observed at 720 nm (1.7 eV) and 560 nm (2.2 eV), and a substantial peak photocurrent response occurs at 395 nm (3.1 eV) and decreases at shorter wavelengths.     

处理器与音视频外设之间的连接

数字信号处理器以需要大量I/O负载的应用为目标,通常为开发人员提供各种集成接口一某些是标准接口,另外一些则是专用接口.例如,美国模拟器件公司(ADI)的Blackfin系列处理器是会聚处理器,这意味着它们在一个芯片上集成了DSP与MCU功能,这些处理器拥有与音频应用相关的两种主要类型的串行接口.该处理器的低比特速率双线接口(TWI)与串行外设接口(SPI)模块是用于音频器件控制与配置的.这些外设接口的前向通道通常用于配置或控制音频转换器,而反向通道则主要负责传输转换器的反馈信息或状态信息.该处理器的高比特速率串行端口外围电路,通常用于执行音频数据传输.     

Electron mobility in an AlGaN/GaN two-dimensional electron gas. I. Carrier concentration dependent mobility

The transport properties of two-dimensional electron gas (2-DEG) at the AlGaN/GaN interface were studied by characterizing the 2-DEG mobility dependence on carrier concentration, n/sub s/, and temperature. High-quality AlGaN/GaN heterostructures were grown, and heterostructure field effect transistors (HFETs) using a Fat FET geometry were fabricated. Measurements of 2-DEG mobility were performed by magnetoresistance and capacitance-conductance. In order to understand the dominant transport factors, the mobility was modeled using different scattering mechanisms and compared to our results. It is found that mobility dependence on n/sub s/ shows a bell-shape behavior over the whole temperature range. For low n/sub s/ the mobility is dominated by Coulomb interaction from interface charge, and at high n/sub s/ the mobility is dominated by interface roughness. Using previously reported experimental values of interface charge and interface roughness in our modeling, we show good agreement with mobility measurement results. Scattering from interface states in AlGaN/GaN heterostructures, seems to be related to the high polarization field in the heterointerface. At temperatures higher than 200K polar optical phonon scattering dominates the transport, yet both interface charge and roughness affect the mobility at the low and high n/sub s/, respectively.     

Templated Crosslinked Imidazolyl Acrylate for Electronic Detection of Nitroaromatic Explosives

A low‐voltage operable, highly sensitive, and selectively responsive polymer for the detection of nitroaromatic explosives is investigated. Resistive devices are fabricated by simple spin‐coating on flexible and transparent substrates in addition to silicon substrates and are stable under ambient temperature and oxygen levels before exposure to the nitroaromatics. After exposure to 2,4,6‐trinitrotoluene (TNT), the devices show increased conductance, even with picogram (pg) quantities of TNT, accompanied by a confirming color change from colorless to deep red. The relative conductance increase per unit exposure is the highest yet reported for TNT. Aromatic anion salts, on the other hand, do not induce any electronic responses. ¹H NMR and microscopic analyses show chemical interactions and morphological changes correlated with the electronic responses, some of which are specific to TNT in relation to other nitroaromatics. The binding constant for the imidazole rings and TNT is on the order of tens of M ^?1. The materials are promising for rapid indication of exposure to nitroaromatic compounds.     

OPM/Web – Object-Process Methodology for Developing Web Applications

Web applications can be classified as hybrids between hypermedia and information systems. They have a relatively simple distributed architecture from the user viewpoint, but a complex dynamic architecture from the designer viewpoint. They need to respond to operation by an unlimited number of heterogeneously skilled users, address security and privacy concerns, access heterogeneous, up-to-date information sources, and exhibit dynamic behaviors that involve such processes as code transferring. Common system development methods can model some of these aspects, but none of them is sufficient to specify the large spectrum of Web application concepts and requirements. This paper introduces OPM/Web, an extension to the Object-Process Methodology (OPM) that satisfies the functional, structural and behavioral Web-based information system requirements. The main extensions of OPM/Web are adding properties of links to express requirements, such as those related to encryption; extending the zooming and unfolding facilities to increase modularity; cleanly separating declarations and instances of code to model code transferring; and adding global data integrity and control constraints to express dependence or temporal relations among (physically) separate modules. We present a case study that helps evaluate OPM/Web and compare it to an extension of the Unified Modeling Language (UML) for the Web application domain.     

A novel application of the infrared coagulator in hair transplantation. Significant reduction of width of the donor site wound

BACKGROUND: The infrared coagulator, a by-product of laser technology, has been used in dermatology in a variety of settings. During hair transplantation sessions, we observed a significant reduction of the donor ellipse width while performing hemostasis with the infrared coagulator. OBJECTIVE: Quantitative assessment of the donor wound width after infrared coagulator use, and correlation to the number of previous transplant sessions and patients' age. METHODS: Twenty-four patients (22 men, two women) underwent hair transplantation. The infrared coagulator was utilized for hemostasis with a pulse duration of 2.5 seconds. RESULTS: The infrared coagulator produced an average donor area decrease of 42%, while achieving rapid hemostasis. No correlation was demonstrated to number of previous transplant sessions or patients' age. CONCLUSIONS: The infrared coagulator significantly decreases the donor wound width while providing hemostasis. Advantages include the potential of larger donor strip harvest, minimal tissue manipulation, and less traumatic closure.     

Regulation of periodontal ligament cell functions by interleukin-1beta

Periodontal ligament (PDL) cells maintain the attachment of the tooth to alveolar bone. These cells reside at a site in which they are challenged frequently by bacterial products and proinflammatory cytokines, such as interleukin-1beta (IL-1beta), during infections. In our initial studies we observed that IL-1beta down-regulates the osteoblast-like characteristics of PDL cells in vitro. Therefore, we examined the functional significance of the loss of the PDL cell's osteoblast-like characteristics during inflammation. In this report we show that, during inflammation, IL-1beta can modulate the phenotypic characteristics of PDL cells to a more functionally significant lipopolysaccharide (LPS)-responsive phenotype. In a healthy periodontium PDL cells exhibit an osteoblast-like phenotype and are unresponsive to gram-negative bacterial LPS. Treatment of PDL cells with IL-1beta inhibits the expression of their osteoblast-like characteristics, as assessed by the failure to express transforming growth factor beta1 (TGF-beta1) and proteins associated with mineralization, such as alkaline phosphatase and osteocalcin. As a consequence of this IL-1beta-induced phenotypic change, PDL cells become responsive to LPS and synthesize proinflammatory cytokines. The IL-1beta-induced phenotypic changes in PDL cells were transient, as removal of IL-1beta from PDL cell cultures resulted in reacquisition of their osteoblast-like characteristics and lack of LPS responsiveness. The IL-1beta-induced phenotypic changes occurred at concentrations that are frequently observed in tissue exudates during periodontal inflammation (0.05 to 5 ng/ml). The results suggest that, during inflammation in vivo, IL-1beta may modulate PDL cell functions, allowing PDL cells to participate directly in the disease process by assuming LPS responsiveness at the expense of their normal structural properties and functions.