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41.
42.
The block copolymers consisting of 2,7-dimethoxycarbazole- and oxadiazole-containing segments as hole and electron transporting units, respectively, were synthesized by NMRP manner. OLED devices were fabricated using block copolymer, random copolymer, and polymer blend for matrix of the emitting layer with Ir(ppy)3 as a phosphorescent dopant in order to investigate morphological effect on the performance. From the finding that the block copolymer system overwhelmed the others in EQE, we assumed that a morphology with dimethoxycarbazole units assembled to the surface of PEDOT:PSS played a considerable role for effective recombination of charges as well as sufficient charge injection into the emitting layer. 相似文献
43.
A photocatalytic sensor for the determination of chemical oxygen demand (COD) using titanium dioxide, based on the use of a pair of oxygen electrodes and flow injection analysis, is described. The measuring principle is based on the direct determination of the oxygen concentration change resulting from photocataltic oxidation of organic compounds. One of the two oxygen electrodes, the reference oxygen electrode, was utilized to measure the reference signal responding only to oxygen present in the injected samples. Oxygen consumption due to the TiO2-catalyzed photochemical oxidation of organic compounds in samples was monitored with the working oxygen electrode. The COD value of this sensor was calculated as the difference of the currents at reference and working oxygen electrodes, respectively. The operation characteristics of the sensor are demonstrated using artificially treated wastewater as a substrate. The sensor was also applied to the determination of COD in real water samples from dam reservoirs (n = 20) all over Japan. The results were in good agreement with those from the conventional COD methods (i.e., permanganate and dichromate methods). 相似文献
44.
To examine the effect of stress on skin homeostasis, cutaneous barrier recovery was measured in rate exposed to immobilization stress after tape stripping or sodium dodecyl sulphate treatment. The barrier function was evaluated by measuring transepidermal water loss. Barrier recovery was delayed in rats exposed to stress in comparison with untreated controls. This tendency was observed in both male and female animals. The delay in barrier recovery was blocked by application of the sedative drugs diazepam and chlorpromazine. The barrier recovery rate in mice which were kept at a high population density (10 animals per cage) for 2 weeks was slower than that in mice kept at lower population densities (five animals or one animal per cage). These animal models could be useful for objectively quantifying the influence of stress on the cutaneous function. 相似文献
45.
Acrylic resin dentures have the potential to elicit irritation, inflammation, and an allergic response of the oral mucosa. Studies of substances leachable from acrylic resins, their cytotoxicity to cultured cells, and means of reducing their leaching were systematically conducted. Under in vivo and in vitro conditions, formaldehyde and methyl methacrylate were significantly leached into human saliva and saliva-substitute buffer, especially from autopolymerized resins. Both leachable substances showed cytotoxic potentials in the range of their leaching concentrations. Formaldehyde was cytotoxic at lower concentrations than methyl methacrylate. Preleaching in water reduced subsequent leaching of both formaldehyde and methyl methacrylate, and the amount of reduction depended on an increase in the preleaching temperatures. Immersion of acrylic resin dentures in hot water (50 degrees C) before insertion is recommended, especially for autopolymerized resins used either for rebasing or as denture base materials, to minimize the risk of adverse reactions in patients who wear acrylic resin dentures. 相似文献
46.
Shinichiro Gondo Shinbu Tanaka Kazuyoshi Kazikuri Koichiro Kusunoki 《Chemical engineering science》1973,28(7):1437-1445
Liquid mixing by large gas bubbles of spherical cup was investigated for co- and counter-current contact of air-water system with bubble columns of 5 and 10 cm dia. The results obtained are that for the column of 5 cm dia., the longitudinal dispersion coefficient ranges from 5 to 20 cm2/sec for superficial gas velocity from 0·07 to 8 cm/sec and that for the one of 10 cm in diameter it ranges from 9 to 45 cm2/sec for that from 0·035 to 8 cm/sec. Liquid mixing under the coexistence of large and small bubbles was also investigated and it was found that the gas holdup was fairly well explained by an equation derived on the assumption that the mixture of small bubbles and liquid behaves independently of large bubbles. The expansion model was applied to the experimental results on the longitudinal dispersion coefficient and it was observed that there should be the lower limit in the holdup of small bubbles where this model can be applied. 相似文献
47.
Acidophilic xylanase from Aureobasidium pullulans: efficient expression and secretion in Pichia pastoris and mutational analysis 总被引:1,自引:0,他引:1
Tanaka H Okuno T Moriyama S Muguruma M Ohta K 《Journal of Bioscience and Bioengineering》2004,98(5):338-343
A yeast-like fungus Aureobasidium pullulans var. melanigenum strain ATCC 20524 produces an extracellular acidophilic endo-1,4-beta-xylanase with an optimum pH of 2.0 [Ohta et al., J. Biosci. Bioeng., 92, 262-270 (2001)]. The xynI cDNA encoding the precursor protein (XynI) was expressed in the methylotrophic yeast Pichia pastoris under the control of the alcohol oxidase I gene promoter. The 34 amino acid prepro-signal peptide of the A. pullulans XynI directed the efficient secretion of 178 mg of active xylanase per liter of the culture medium. The secretion level of the xylanase with its own signal peptide was comparable to that of the mature protein fused to the prepro leader from Saccharomyces cerevisiae alpha-mating factor and twofold higher than that of the mature protein fused to the pre-type signal peptide from P. pastoris acid phosphatase. The N-terminal amino acid sequence and the apparent M(r) of 24 kDa of the secreted recombinant protein indicated the native-like processing of the A. pullulans XynI signal sequence in P. pastoris. The three-dimensional model and mutational analysis of the xynI gene product showed that Asp-73 and Glu-157 residues located at the upper and lower edges of the active site cleft, respectively, play a significant role in its low pH optimum. 相似文献
48.
Kojima K Hiratsuka A Suzuki H Yano K Ikebukuro K Karube I 《Analytical chemistry》2003,75(5):1116-1122
An electrochemical protein chip was microfabricated. A thin-film three-electrode system, including an array of 36 platinum working electrodes, a set of thin-film Ag/AgCl electrodes, and platinum auxiliary electrodes, was integrated on a glass substrate. Capture antibodies were immobilized in a 4.5-nm-thick double layer of a hexamethyldisiloxane plasma-polymerized film. Because of their highly cross-linked network structure, the capture antibodies could be firmly immobilized. No nonspecific adsorption was observed during a series of procedures to detect target proteins, and electrochemical cross talk between neighboring sites was negligible. The sandwich immunoassay was conducted on a single chip using model proteins, alpha-1-fetoprotein and beta2-microglobulin. A distinct current increase following the oxidation of hydrogen peroxide produced by the enzymatic reaction of glucose oxidase was observed, which indicates that the capture proteins could actually bind the target proteins. Two kinds of protein were detected independently on multiple sites with respective capture antibodies. 相似文献
49.
Fujikake H Yonai J Sato H Kikuchi H Iino Y Kawakita M Tsuchiya Y Iwashita K Umeda S 《Applied optics》2001,40(14):2275-2281
A color-temperature compensating system with an electrically controllable liquid-crystal filter and a color sensor mounted on a video camera has been developed for color image sensing. The filter contains two guest-host liquid-crystal devices with dichroic dyes that have strong light absorption for shorter-wavelength light; two devices are necessary because of the spectral difference between the sun and an incandescent lamp as light sources. The filter's absorption is continuously controlled by the voltage applied to the filter. Because the filter is driven according to spectral information about the illumination detected by the color sensor, the color balance of the video image to be sensed can be compensated automatically and rapidly. This is especially useful for video image shooting in which a video camera experiences changes in illumination color temperatures. 相似文献
50.
This paper reports the fabrication process of β-FeSi2 droplets on silicon substrates at room temperature by ArF excimer pulsed laser deposition (PLD). The chemical treatment of substrate could compensate the thermal treatment of the deposited droplets. Observations with the transmission electron microscopy revealed that the crystallization of droplet began from the surface of droplet rather than from the interface between the melt and the substrate. 相似文献