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排序方式: 共有639条查询结果,搜索用时 15 毫秒
61.
J Morokuma K Akasaka K Mitsunaga-Nakatsubo H Shimada 《Canadian Metallurgical Quarterly》1997,39(4):469-476
Mycobacteria generally have thick cell walls and contain large amounts of lipid, making them resistant to DNA extraction. Five methods, namely, extensive enzymic digestion method (M1), 2-min mechanical glass-bead disruption method (M2), thermal shock method (M3), modified conventional enzymic digestion method (M4), and manual disruption with modified conventional enzymic digestion method (M5), were used to compare their effectiveness and simplicity in extracting DNA from slowly growing mycobacteria (Mycobacterium leprae, M. lepraemurium and M. bovis BCG), and a rapidly growing mycobacterium (M. phlei). The highest DNA yield was obtained by M2 from M. lepraemurium which produced 2.82 micrograms DNA/mg wet weight of cells, representing a theoretical yield of 78%. M3 gave the lowest DNA yield; 0.01 microgram DNA/mg wet weight of cells of M. lepraemurium was obtained. M4, in which proteinase K was used, is more effective than M1, in which subtilisin and pronase were used. M5 yielded a higher amount of DNA, but it required more manipulations to extract DNA as compared to M4. Extraction of DNA of M. leprae from nude mice is more difficult than that of M. leprae from armadillos by all of the methods used. These results suggest that the biosynthetic capabilities of these two forms of M. leprae may vary, depending on their cultural conditions and/or strain differences. Our results have shown that both M2 and M4 are the simplest, most effective and time-saving methods which are suitable for every routine laboratory to extract DNA from slowly and rapidly growing mycobacteria. 相似文献
62.
Manabu Iguchi Tsuneo Kondoh Zen-Ichiro Morita Keiji Nakajima Kazuharu Hanazaki Tomomasa Uemura Fujio Yamamoto 《Metallurgical and Materials Transactions B》1995,26(2):241-247
Laser Doppler velocimeter (LDV) measurements were made to clarify the fluid flow behavior in a bath subject to centric bottom
gas injection. Correlations of the axial mean velocity and turbulence components in the gas-liquid two-phase flow region,i.e., in the bubbling jet region, were proposed as functions of the inner diameter of nozzle, gas flow rate, and densities of
gas and liquid. Measured values of the flow rate, momentum, and kinetic energy of water rising upward were approximated satisfactorily
by these empirical correlations. In addition, the Reynolds shear stress was calculated and compared with measured values.
Formerly Graduate Student, Osaka University, 相似文献
63.
Ragnhild E. Aune Miyuki Hayashi Keiji Nakajima Seshadri Seetharaman 《JOM Journal of the Minerals, Metals and Materials Society》2002,54(11):62-69
The optimization of metallurgical processes requires reliable data of the slag phase. This paper focuses on three properties
that are relevant to heat and mass-transfer calculations — viscosities,thermal diffusivities, and surface tensions of silicate melts. A brief account of the experimental techniques used for the
measurements of these properties, with special reference to the work carried out in the Division of Metallurgy, Royal Institute
of Technology, Stockholm, Sweden, are presented, along with the advantages and limitations. As these properties are structure-oriented,
the impact of structure on these properties is also presented. The paper is intended as a state-of-the-art review of the subject. 相似文献
64.
James A. Cheshire Paul A. Longley Keiji Yano Tomoki Nakaya 《Papers in Regional Science》2014,93(3):539-555
This paper uses an extended case study of Japan to illustrate how surnames, or family names, can be used as a basis for regionalization. We undertake a comparison between inductively surname regions of Japan with areal geographies based upon both contemporary and historical prefecture (administrative) units. The work is seen as using highly disaggregate framework data to evaluate the integrity of the areal units that are used in regional science. It also is relevant to understanding population distributions, past and present, and the consequences of local, regional and national residential mobility and migration. 相似文献
65.
Tie?Liu Qiang?WangEmail author Hong-Wei?Zhang Chang-Sheng?Lou Keiji?Nakajima Ji-Cheng?He 《Journal of Materials Science》2011,46(6):1628-1634
The effects of high magnetic fields on the solidification microstructure of Al–Si alloys were investigated. Al–7.2 wt%Si and
Al–11.8 wt%Si alloys were solidified in various high magnetic fields at different cooling rates. The secondary dendrite arm
spacing (SDAS) of the primary Al dendrites and the lamellar spacing (LS) of the eutectics were measured. It was found that
the application of a high magnetic field could decrease the SDAS of the primary Al dendrites in Al–7.2 wt%Si alloys and the
LS of the eutectics in Al–11.8 wt%Si alloys. The effects of the high magnetic field on the SDAS decreased with increasing
cooling rate. The decrease in the SDAS and LS can be attributed to the decrease of the solute diffusivity in the liquid ahead
of the solid/liquid interface during the growth of the dendrite and eutectic. This decrease is caused by the high magnetic
field which can damp the convection and avoid its contributions to the diffusion. 相似文献
66.
Variation of Fructooligosaccharides and their Metabolizing Enzymes in Onion Bulb (Allium cepa L. cv. Tenshin) During Long-term Storage 总被引:1,自引:0,他引:1
Noureddine Benkeblia Keiji Ueno Shuichi Onodera Norio Shiomi 《Journal of food science》2005,70(3):S208-S214
ABSTRACT: The objective of this study was to assess the status of fructooligosaccharides (FOS) in onion bulbs ( Allium cepa L. cv. Tenshin) and their metabolizing-enzymes—1-fructoexohydrolase (1-FEH), 1-kestose hydrolyzing enzyme (1-KH), fructan:fructan 1F -fructosyltransferase (1-FFT) and fructan:fructan 6G -fructosyltransferase (6G-FFT)—during storage at 15°C. Fructose varies slightly, whereas 1-kestose peaked after 6 wk and then decreased progressively during the last 18 wk of storage. Lower degree of polymerization (DP) 3 to 6) FOS, higher (DP 7 to 12) FOS, total FOS, and total carbohydrates showed similar and close patterns during 24 wk. They varied slightly at the beginning of the storage period; afterward they decreased progressively and regularly during the last 20 wk of storage. 1-FEH and 1-KH activities were low but peaked abruptly after 12 and 16 wk, respectively, after which they decreased to levels higher (1-FEH) or similar (1-KH) to those observed at the beginning of the storage. Surprisingly, 1-FFT activity showed similar pattern to the variation of 1-KH hydrolyzing activity; on the other hand, 6G-FFT, although higher, was stable during 16 wk but decreased after that. The results allowed us to associate FOS to the dormancy and sprouting states, and the peaks of the degrading enzymes were shown to signal the release of dormancy of onion bulb. 相似文献
67.
Reforming of Methane with Carbon Dioxide over a Catalyst Consisting of Ruthenium Metal and Cerium Oxide Supported on Mordenite 总被引:1,自引:0,他引:1
Reforming of CH4 with CO2 proceeds at 400 °C over a catalyst consisting of ruthenium metal and CeO2 highly dispersed on mordenite. The catalyst, Ru-CeO2/MZ, is highly active for the reforming of CH4 under the conditions at which a carbon formation reaction is thermodynamically apt to take place. The reforming selectively forms H2 and CO. An increase in the weight of the catalyst resulting from carbon deposits was scarcely observed. IR spectra for the catalyst indicate that the reforming proceeds via the formation of the intermediate species such as Ru-CO and Ru-CHx on the surface of ruthenium. The data of H2 adsorption support the idea that ruthenium is highly dispersed in Ru-CeO2/MZ. 相似文献
68.
Keiji Nakajima Hajime Hasegawa Sakhob Khumkoa Shozo Mizoguchi 《Metallurgical and Materials Transactions B》2003,34(5):539-547
In order to elucidate the nature of the heterogeneous nucleation, a differential scanning calorimetry (DSC) thermal analysis
of pure Fe and Fe-Ni alloys (Ni content: 1.0 to 29.3 mass pct) containing TiN, Al2O3, and Ti2O3 was conducted. Then, special attention was paid to the difference in the phase of the primary crystal nucleated by the triggering
effect of a catalyst (nucleating agent). The solidification and transformation mode appearing during cooling in these alloys
is classified into three cases: F mode, FA mode, and A mode. The change of modes and the critical undercooling (ΔT) depend on the kind of catalyst used as well as the chemical composition (Ni content). In addition, in spite of the kind
of primary crystal, the value of ΔT is always small in the order of TiN, Al2O3, and Ti2O3. As a matter of fact, only TiN has a practical effect as a catalyst on the triggered nucleation of the primary crystal of
the δ phase. None of them has a practical effect on the nucleation of the primary crystal of the γ phase.
This article is based on a presentation given in the Mills Symposium entitled “Metals, Slags, Glasses: High Temperature Properties
& Phenomena,” which took place at The Institute of Materials in London, England, on August 22–23, 2002. 相似文献
69.
Alumina has been incorporated in the cavities of mordenite by the hydrolysis of Al3+ ions in the cavities with moist ammonia gas, following calcination at 450°C. The incorporation of the superfine particles of alumina into the cavities of the mordenite is assessed on the basis of the data of MAS 27Al NMR measurements, X-ray powder diffraction and the amount of saturated adsorption of nitrogen for FAL/MZ. The acid properties, assessed using NH3 TPD and FTIR studies, show the presence of Brønsted and Lewis acid sites on the catalyst, and an increase in acidic sites on incorporating alumina. The dehydrogenation selectivities of FAL/MZ, Al3+/MZ, H+/MZ, and Na+/MZ have been investigated. Brønsted acid sites largely enhance the ring-cleavage, whereas this incorporation of superfine particles of alumina significantly suppresses the ring-cleavage. Therefore, the incorporation of the superfine particles increases remarkably in the cyclohexene selectivity. 相似文献
70.