Effect of Nucleation Temperature on Detecting Molecular Ions and Charged Nanoparticles with a Diethylene Glycol-Based Particle Size Magnifier

The classical nucleation theory predicts that a decrease in nucleation temperature under a constant saturation ratio increases the energy barrier for homogeneous nucleation to occur; therefore lower nucleation temperature would allow higher saturation ratio inside a condensation particle counter (CPC) while suppressing homogenous nucleation of working fluid vapor below a threshold value. On the other hand, the classical theory also predicts that a decrease in nucleation temperature increases the energy barrier for heterogeneous nucleation to occur, which potentially increases the minimum detectable size of CPC. Accordingly, it is important to investigate experimentally whether higher super-saturation under lower nucleation temperature decreases the minimum detectable size or not. Minimum detectable sizes of a diethylene glycol (DEG)-based nanoparticle size magnifier (nano-PSM) developed by Ito et al. (Ito, E., Seto, T., Otani, Y., and Sakurai, H. [2011]. Aerosol Sci. Technol. 45:1250–1259) were investigated at three different nucleation temperatures. Mobility standard molecular ions and mobility-classified silver nanoparticles were used as test aerosol particles. The vapor flux exiting from the evaporator in nano-PSM is accurately controlled using a syringe pump. The temperature of the mixing region and condenser in the nano-PSM is controlled at adiabatic mixing temperature, which we call its nucleation temperature. When the nucleation temperature was set at 16.7, 25.0, and 33.3°C, the minimum detectable mobility diameter values, which were defined at 50% value of counting efficiency curve, were 2.2, 2.9, and 3.0 nm, respectively, indicating that lower nucleation temperature is favorable for detecting smaller sized particles using DEG-based PSM or CPCs.

Copyright 2015 American Association for Aerosol Research     

Drift of amplification factor and its effects in a tri-electrode electron gun†

The drift of the amplification factor p. should induce drifts of the emitted current I a in a fixed-bias circuit and the beam energy in a self-bias circuit.

The μ-drift due to the heat from the filament was measured, and also calculated from the I _A-drift for an hour. The relative μ-drift thus calculated was 1-3% in 10?min in a conventional gun, and it decreased to smaller than 003% in 10?min in an improved gun, where thermal expansion of the Wehnelt electrode was compensated by the movement of the filament supporter. Energy of the beam was 20 kev. The initial emitted current I _A(t= 0) was 198 μA in the conventional gun and 14.4 μA in the improved gun.

The relative energy-drift should be proportional to the relative μ-drift and inversely proportional to (μ+ 1). Accordingly, the relative energy-drift should be 1.3 × 10^?6; in 10?min in the conventional gun, while it should be smaller than 3 ×10^?6 in 10?min in the improved gun, when μ is assumed to be 100 in both guns.     

Preparation of Highly Crystallized Strontium Titanate Powders at Room Temperature

Highly crystallized fine powder of strontium titanate (SrTiO₃) was obtained at room temperature simply by leaving a vial containing the powder mixture of Sr(OH)₂·8H₂O and hydrous titania gel (TiO₂·nH₂O) for 10 d. Solvent and additive(s) used in the conventional low‐temperature process were not used. The crystallinity judged from the FWHM of X‐ray diffraction peak was comparable to that obtained by a solid‐state reaction. To cause the reaction at room temperature, the titania gel had to contain a considerable amount of H₂O (n > 0.97). The reaction is considered to be neutralization of titanic acid [H₄TiO₄ or Ti(OH)₄] and strontium hydroxide (base). Using similar process, highly crystallized BaTiO₃ powder was also obtained at 60°C. In comparison with the formation temperature of BaTiO₃, tolerance factor in the perovskite structure was important for the room‐temperature synthesis of SrTiO₃. SrTiO₃ was hardly obtained at room temperature by the addition of saturated strontium hydroxide solution to the hydrous TiO₂ gel (n = 1.29). Therefore, the reaction seems to proceed in the hydrous titania gel. This process is characterized by three important points; “no solvent”, “no additional reagents”, and above all, “no heating”.     

Experimental analysis in recording transmission and reflection holograms at the same time and location

Holographic recording media with a reflection layer are useful because they make it possible to maintain backward compatibility with CDs and DVDs, and a conventional servo system is easily attachable. The incident beam is fed back to the recording layer by the reflection layer, so there are four beam pairs to record the transmission and reflection holograms. We analyze the basic property of the transmission and reflection holograms and evaluate the problem when the transmission and reflection holograms are recorded at the same time. It is shown that the shrinkage in the photopolymer medium has a different effect on each hologram, so the readout image from the two holograms is misaligned. Those diffraction beams make the interference pattern, and the signal-to-noise ratio (SNR) of the output image decreased. Taking into account the difference in wavelength selectivity between the transmission and the reflection holograms, we propose a way to select one hologram to get the diffraction beam and eliminate the interference pattern using the tuning readout wavelength. By using this method, we can eliminate the diffraction beam from the reflection hologram and keep a high SNR.     

Near-infrared fluorescence probes for enzymes based on binding affinity modulation of squarylium dye scaffold

We present a novel design strategy for near-infrared (NIR) fluorescence probes utilizing dye-protein interaction as a trigger for fluorescence enhancement. The design principle involves modification of a polymethine dye with cleavable functional groups that reduce the dye's protein-binding affinity. When these functional groups are removed by specific interaction with the target enzymes, the dye's protein affinity is restored, protein binding occurs, and the dye's fluorescence is strongly enhanced. To validate this strategy, we first designed and synthesized an alkaline phosphatase (ALP) sensor by introducing phosphate into the squarylium dye scaffold; this sensor was able to detect ALP-labeled secondary antibodies in Western blotting analysis. Second, we synthesized a probe for β-galactosidase (widely used as a reporter of gene expression) by means of β-galactosyl substitution of the squarylium scaffold; this sensor was able to visualize β-galactosidase activity both in vitro and in vivo. Our strategy should be applicable to obtain NIR fluorescence probes for a wide range of target enzymes.     

Numerical simulation of buoyancy-assisting,backward-facing step flow and heat transfer in a rectangular duct

Two- and three-dimensional numerical simulations have been performed for mixed convective upward flows over a backward-facing step in a duct. The Reynolds number, expansion ratio, and aspect ratio (in 3-D simulations) were kept constant at Re = 125, ER = 2, and AR = 16, respectively. The heat flux at the wall downstream of the step was uniform. The straight wall, the step, and the side walls (in 3-D simulations) were assumed to be adiabatic. The effect of the buoyancy level, Ri^*, was the major interest in this study. It was found that the reattachment point and the peak Nusselt number point moved upstream as Ri^* was increased. The secondary recirculation region, which developed at the corner of the step, became larger. A secondary flow was also found in a cross section immediately downstream of the step. Flow directed toward the center of the duct became more intensive as Ri^* increased, which possibly resulted from an increase in the level of three-dimensionality of the flow and thermal fields. © 1999 Scripta Technica, Heat Trans Asian Res, 28(1): 58–76, 1999     

Three-dimensional monolithic microwave integrated circuit technology for fully computer-aided design-compatible monolithic microwave integrated circuit development

This paper presents the advantages of the three-dimensional (3D) masterslice monolithic microwave integrated circuit (MMIC) technology and the application of its technology to computer-aided design (CAD) software. Its high level of freedom in transmission line routing offers design flexibility and the high integration of 3D MMICs. A 20-GHz single-chip receiver and 60 GHz amplifier are demonstrated. The 3D masterslice MMIC technology, an advanced MMIC design method, is effective in making existing CAD software fully CAD compatible by attaching software packages which allow the sharing of parameters between schema and layout, autorouting, customized simulation and database including reference circuits, and so on. Masterslice MMIC CAD software is also developed. © 1998 John Wiley & Sons, Inc. Int J RF and Microwave CAE 8: 498–506, 1998.     

Dual Nature of RAGE in Host Reaction and Nurturing the Mother–Infant Bond

Non-enzymatic glycation is an unavoidable reaction that occurs across biological taxa. The final products of this irreversible reaction are called advanced glycation end-products (AGEs). The endogenously formed AGEs are known to be bioactive and detrimental to human health. Additionally, exogenous food-derived AGEs are debated to contribute to the development of aging and various diseases. Receptor for AGEs (RAGE) is widely known to elicit biological reactions. The binding of RAGE to other ligands (e.g., high mobility group box 1, S100 proteins, lipopolysaccharides, and amyloid-β) can result in pathological processes via the activation of intracellular RAGE signaling pathways, including inflammation, diabetes, aging, cancer growth, and metastasis. RAGE is now recognized as a pattern-recognition receptor. All mammals have RAGE homologs; however, other vertebrates, such as birds, amphibians, fish, and reptiles, do not have RAGE at the genomic level. This evidence from an evolutionary perspective allows us to understand why mammals require RAGE. In this review, we provide an overview of the scientific knowledge about the role of RAGE in physiological and pathological processes. In particular, we focus on (1) RAGE biology, (2) the role of RAGE in physiological and pathophysiological processes, (3) RAGE isoforms, including full-length membrane-bound RAGE (mRAGE), and the soluble forms of RAGE (sRAGE), which comprise endogenous secretory RAGE (esRAGE) and an ectodomain-shed form of RAGE, and (4) oxytocin transporters in the brain and intestine, which are important for maternal bonding and social behaviors.     

Characterization of the aramid/epoxy interfacial properties by means of pull-out test and influence of water absorption

Single fiber pull-out tests were carried out to investigate the influence of water absorption on the interfacial properties of aramid/epoxy composite. The fiber/matrix interfacial strength was severely decreased between 4 and 7 week immersion time in deionized water at 80 °C, and thereafter showed a plateau. This change with immersion time did not correspond with that of the water gain of the pull-out specimens, because the water gain did not reflect the one in the fiber/matrix interface. As a result of the degradation of the fiber/matrix interfacial strength, the pulled-out fiber surfaces of 7, 10 and 13 week wet specimen were smooth. In situ observations of interfacial crack propagation by a video microscope and an analysis of acoustic emission (AE) signals showed that AE signals obtained during the pull-out process were classified into four types according to fracture modes. AE signals detected at final unstable crack propagation and fiber breakage had high amplitude and long duration.     

Relaxation behavior of polymers probed by positron annihilation lifetime spectroscopy

Relaxation behaviors of polyethylene, polypropylene, and polycarbonate have been studied by positron annihilation lifetime spectroscopy (PALS). In PALS positron sources made of radioisotopes are used to inject positrons into polymer as a micro probe. The injected positron probes can induce radiation effect, which plays an important role in detecting the polymer relaxation behavior through electrons trapped in shallow potentials at low temperature. Monitoring the intensity (I₃) of ortho-positronium (o-Ps), transitions of γ and δ relaxation can be measured by PALS as a secondary effect. In this experiment, the change of I₃ below Tg is connected with the number of the trapped electrons, which can be excited from the shallow potential by the thermal motion of polymer structures and visible light irradiation. In the PALS measurements of non-irradiated PP samples, relaxation of methyl groups was observed as low as 50 K, which can be assigned as the δ relaxation. Relaxations of β and γ were also observed for the non-irradiated PP samples between 100–370 K. However for the 3 MGy γ-ray irradiated PP samples, only β relaxation was observed because the large radiation dose caused a large number of scissions of –CH₃ groups from main chains and the characteristics changed. For the irradiated samples, radiation hardening was observed. Electronic Publication