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The hepatitis C virus (HCV) is a single‐stranded (ss) RNA virus that is responsible for chronic liver diseases, such as cirrhosis, end‐stage liver disease, and hepatocellular carcinoma. Driven by the need to detect the presence of the HCV viral sequence, herein it is demonstrated for the first time that the nonlinear optical (NLO) properties of gold nanoparticles can be used for screening and quantifying HCV RNA without any modification, with excellent detection limit (80 pM ) and selectivity (single base‐pair mismatch). The hyper‐Rayleigh scattering (HRS) intensity increases 25 times when label‐free, 145‐mer, HCV ss‐RNA is hybridized with 400 pM target RNA. The mechanism of HRS intensity change is discussed with experimental evidence for a higher multipolar contribution to the NLO response of gold nanoparticles.  相似文献   
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The utilization of motor proteins for the movement and assembly of synthetic components is currently a goal of nanoengineering research. Application of the myosin actin motor system for nanotechnological uses has been hampered due to the low flexural rigidity of individual F‐actin filaments. Here it is demonstrated how actin bundling can be used to affect the translational behavior of myosin‐propelled filaments, transport molecules across a motor‐patterned surface, and that the movement of bundled actin can be regulated photonically. These data suggest that actin bundling may significantly improve the applicability of the myosin motor for future nanotechnological applications.  相似文献   
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The radial mechanical properties of single-walled boron nitride nanotubes (SW-BNNTs) are investigated by atomic force microscopy. Nanomechanical measurements reveal the radial deformation of individual SW-BNNTs in both elastic and plastic regimes. The measured effective radial elastic moduli of SW-BNNTs are found to follow a decreasing trend with an increase in tube diameter, ranging from 40.78 to 1.85 GPa for tube diameters of 0.58 to 2.38 nm. The results show that SW-BNNTs have relatively lower effective radial elastic moduli than single-walled carbon nanotubes (SWCNTs). The axially strong, but radially supple characteristics suggest that SW-BNNTs may be superior to SWCNTs as reinforcing additives for nanocomposite applications.  相似文献   
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Organic semiconductor films are susceptible to noncovalent interactions, trapping and doping, photoexcitation, and dimensional deformation. While these effects can be detrimental to the performance of conventional circuits, they can be harnessed, especially in field‐effect architectures, to detect chemical and physical stimuli. This Review summarizes recent advances in the use of organic electronic materials for the detection of environmental chemicals, pressure, and light. The material features that are responsible for the transduction of the input signals to electronic information are discussed in detail.  相似文献   
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A recent trend in technological innovation is towards the development of increasingly multifunctional and complex products to be used within rich socio‐cultural contexts such as the high‐end office, the digital home, and professional or personal healthcare. One important consequence of the development of strongly innovative products is a growing market uncertainty regarding ‘if’, ‘how’, and ‘when’ users can and will adopt such products. Often, it is not even clear to what extent these products are understood and interacted with in the intended manner. The mentioned problems have already become an evident concern in the field, where there is a significant rise in the numbers of seemingly sound products being complained about, signaling a lack of soft reliability. In this paper, we position soft reliability as a growing and critical industrial problem, whose solution requires new academic expertise from various disciplines. We illustrate potential root causes for soft reliability problems, such as discrepancy between the perceptions of users and designers. We discuss the necessary approach to effectively capture subjective feedback data from actual users, e.g. when they contact call centers. Furthermore, we present a novel observation and analysis approach that enables insight into actual product usage, and outline opportunities for combining such objective data with the subjective feedback provided by users. Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   
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