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51.
Comparison of the effect of a proteolytic enzyme dispase and a chelator for the preparation of epidermal sheet from nude mice. 总被引:1,自引:0,他引:1
Histochemical studies of epidermal Langerhans cells require well-preserved epidermal sheets. We studied the conditions for the preparation of the epidermal sheets from the skin of the ear, hind limb and trunk of nude mice. Two types of commercial dispase, and ethylenediaminetetraacetic acid (EDTA), were used at several concentrations and the effects were compared. Dermo-epidermal separation was evaluated by the preservation of ATPase activity of Langerhans cells in the epidermal sheet and by the ultrastructure of epidermal cells (Langerhans cells and keratinocytes) as well as epidermal-dermal junction. Good separation depended on the combination of the concentration of the reagent and site of the skin. The concentrations of both dispase and EDTA effective for separation of the epidermis in the nude mouse were lower than those in other mouse strains reported. 相似文献
52.
I Kobayashi M Hasegawa S Miyazaki M Nishida S Goto 《Canadian Metallurgical Quarterly》1993,67(6):528-534
Pseudomonas aeruginosa Nos. 1 and 5, each co-existing growth-inhibitor-producing and -nonproducing cells, were used in this study. An equal number of both cells (each 10(8) CFU/mouse) was challenged intraperitoneally to mice, and these cells in the heart blood and kidneys of mice were determined. Furthermore, the effect of piperacillin, ceftazidime and sisomicin on the cell distribution in mice was studied in the model infection due to P. aeruginosa Nos. 1 and 5. As a control experiment both cells of P. aeruginosa No. 1 were each challenged intraperitoneally at a dose of 10(8) CFU/mouse to mice of two groups, but there were no marked differences between the two types in cell counts of the heart blood or kidneys 9 hours after challenge. When a concomitant challenge of both cells (each 10(8) CFU/mouse) was performed in mice, the number of growth-inhibitor-producing cells of the heart blood and kidneys was about 100 times greater than that of the non-producing cells. These in vivo results were well comparable to the previous in vitro results and indicated that the inhibitor affected the invasion of the non-producing bacteria in the body in the model infection due to P. aeruginosa isolates consisting of the two types of cells. Similar results were obtained in mice with the model infection due to P. aeruginosa No. 5. Anti-pseudomonal drugs such as piperacillin (50 mg/mouse) and ceftazidime (50 mg/mouse) and sisomicin (1 mg/mouse) were given intramuscularly to mice infected concomitantly with both cells of P. aeruginosa No. 1.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
53.
54.
S Tanihara T Zhang T Ojima Y Nakamura H Yanagawa M Kobayashi 《Canadian Metallurgical Quarterly》1997,44(9):688-693
A secondary medical area is defined as a medical administrative area under the Medical Care Law by which every prefectural government is expected to provide general medical care supplies such as beds for inpatients. This study evaluated the distribution in medical supplies among the secondary medical areas in Japan and the correlation between medical supply and demand. The numbers of hospital inpatients (residence of patients), physicians, and general beds excluding those for mental disorders and tuberculosis were analysed in relation to the population in all secondary medical areas in Japan. Those data were obtained from surveys conducted by the Ministry of Health and Welfare. The main results were as follows; 1) The distribution of physicians and general hospital beds among the secondary medical areas was not equal. 2) Even adjusted for the sex and age distribution, the highest hospital inpatients/population ratio among the secondary medical areas was four times as large as that of the lowest. 3) With multiple regression models, two independent variables (normal hospital beds/population ratio and aged 65 and over/total population ratio) accounted about 60% of the variance in hospital inpatients/population ratio. 4) The differences in medical supplies among the secondary medical areas may cause the differences in hospital inpatients/population ratio. 相似文献
55.
Tada Y. Kobayashi Y. Yamabayashi Y. Matsuoka S. Hagimoto K. 《Selected Areas in Communications, IEEE Journal on》1996,14(5):914-922
Photonic networks based on wavelength division multiplexing (WDM) and optical path technologies are expected to realize flexible, transparent, and cost-effective transport networks with a large transmission capacity. This paper explores the design framework of photonic transport networks taking into consideration the operation administration and maintenance (OA&M) functions required for the successful introduction of WDM systems based on the optical path concept. From the view point of network maintenance, clear distinction is made between the optical path layer and the optical section layer to facilitate accurate and smooth failure localization. The digital multiplexing span between physical multiplexing interfaces at the end-to-end digital nodes should have the same maintenance span as the corresponding optical path. We argue that cooperative maintenance by OA&M functions at both the digital and optical layers can be a practical way of network supervision. A supervisory (SV) signal transfer method and a configuration that is suitable for the terrestrial trunk network are also indicated. As an example, a practical SV system design methodology and an actual procedure developed for a single channel optical transmission system based on optical in-line amplifiers are introduced. Furthermore, application of the developed SV system and network restoration schemes is discussed for future WDM-based photonic networks. The OA&M aspects introduced will be valuable for creating future photonic network systems 相似文献
56.
The influence of multi-coatings of LaB6-Si/polycarbosilane/SiO2 on the oxidation behaviour of carbon/carbon composite materials was investigated in the temperature range from 500 to 1400 °C. The additives of LaB6-Si offered lower oxidation rates and accelerated increases in oxidation rates at temperatures below 900 °C. The coating of polycarbosilane (PCS) improved the compatibility of the coating on the carbon/carbon composite and lowered the oxidation rates of the LaB6-Si coated composite below the transition temperature. With the SiO2 coating, the cracks of the LaB6-Si/PCS coating was sealed and a good oxidation resistance of the LaB6-Si/PCS/SiO2 coated composite was found at temperatures up to 1300 °C. 相似文献
57.
H Saitoh T Ono A Nomura Y Iwasaki N Ohno S Matsumoto Y Kobayashi H Atarashi T Katoh H Kishida H Hayakawa 《Canadian Metallurgical Quarterly》1998,46(10):1043-1048
Although diagnosis of neurally mediated syncope (NMS) using head-up tilt (HUT) test has been established, the exact mechanism of NMS has not yet been elucidated. We evaluated beta and alpha-adrenergic function in NMS patients by pharmacological autonomic function test. The alpha-adrenergic sensitivity of NMS patients was significantly lower than that of control subjects. The patients who need low dose isoproterenol for provocation of syncope showed higher beta-adrenergic sensitivity than patients who developed syncope without isoproterenol. Thus, pharmacological autonomic function test was useful for evaluation of NMS patients. 相似文献
58.
59.
K Kubota M Makuuchi T Kobayashi Y Sakamoto T Noie K Inoue Y Harihara T Takayama 《Canadian Metallurgical Quarterly》1998,45(20):545-546
When reconstructing the portal vein (PV) following hepatopancreatoduodenectomy (HPD) with PV resection, a new porto-systemic bypass (PSB) technique can be employed to prevent intestinal vascular congestion. The Whipple procedure is performed in a standard manner, as long a portion of the gastrocolic trunk is preserved for insertion of an antithrombogenic catheter (ATC). After harvesting the left external iliac vein and exposing the right great saphenous vein, the end of the ATC is inserted in the superior mesenteric vein via the gastrocolic trunk in the distal direction and the other end of the ATC is inserted in the greater saphenous vein. PSB is achieved as a result of the venous pressure gradient. By employing this technique, an ATC can be inserted without damaging another mesenteric venous branch and with minimal damage to the endothelium, and the small intestine is not exposed in the operative field until enteric reconstruction is started. This technique is a promising option for PSB during HPD with PV resection. 相似文献
60.
The present paper concerns robust H∞ performance for linear delay-differential systems which involve an uncertain time delay and time-varying norm-bounded parameter uncertainties. Based on the Lyapunov functional, a simple criterion is proposed which assures the pseudo-quadratic stability as a well as an H∞-norm bound. The criterion is given in the form of a linear matrix inequality which is affine or convex in additional scalar parameters. A simple criterion is presented to evaluate the extent of the performance robustness 相似文献