Semiautomatic leakage analyzing system for time series fluorescein ocular fundus angiography

An algorithm for measuring an index of leakage in fluorescein fundus angiography is presented. The number of pixels whose gray level exceeds an automatically determined slice level is calculated. An automatic blood vessel elimination technique is introduced. Further, a method of measuring the short term change of leakage from a time series of the fluorescein fundus angiographs is described. Our system can also deal with the time series images obtained on different occasions (long term change).The features of this paper are: (1) this paper deals with double time sequence images (short and long term); (2) an automatic blood vessel recognition algorithm is employed.     

Aerodynamics of high speed trains passing by each other

A three-dimensional flow field induced by two trains passing by each other inside a tunnel is studied based on the numerical simulation of the three-dimensional compressible Euler/Navier-Stokes equations formulated in the finite difference approximation. A domain decomposition method with the FSA (fortified solution algorithm) interface scheme is used to treat this moving-body problem. The computed results show the basic characteristics of the flow field created when two trains pass by each other. The history of the pressure distributions and the aerodynamic forces acting on the trains are the main areas discussed. The results indicate that the phenomenon is complicated due to the interaction of the flow induced by the two trains. Strong side forces occur between the two trains when the front portion of the opposite train passes by. The forces fluctuate rapidly and the maximum suction force occurs when two trains are aligned side by side. The results also indicate the effectiveness of the present numerical method calculating moving boundary problems.     

Influences of interfacial bonding strength and scatter of fibre strength on tensile behaviour of unidirectional metal matrix composites

The influences of interfacial bonding strength and scatter of strength of fibres on tensile behaviour of unidirectional metal matrix composites, whose matrix has low yield stress in comparison to the strength of fibres, were studied using the Monte-Carlo simulation technique using two-dimensional model composites. The following results were found. The strength of composites increases with increasing bonding strength, especially when the bonding strength exceeds the shear yield stress of the matrix and then remains nearly constant. The strength of composites is very sensitive to bonding strength when the scatter of fibre strength is large, but not when it is small. The fracture mode varies from non-cumulative to cumulative with increasing scatter of fibre strength for both cases of weak and strong interfacial bondings. The fracture surface becomes irregular when bonding strength becomes low and scatter of fibre strength becomes large. The applicability of the Rosen and Zweben models and the rule of mixtures to predict the strength of composites was examined.     

Surface oxide structures on a commercial activated carbon

Direct transmission IR spectra of a commercial activated carbon (Filtrasorb 200, Calgon Corporation) as well as spectra of this carbon after neutralization with 0.25 N solutions of NaOH and HCl are presented. Neutralization capacities for bases of different strengths (NaHCO₃, Na₂C0₃ and NaOH) indicate that the majority of the acidic surface groups (65%) are of weak acidity. This carbon adsorbs acid as well. From the analysis of the spectra it is concluded that the main oxides present on the surface of this particular carbon are lactones, quinones, phenols and carboxylates. The lactonic structures open upon reaction with alkali to give carboxylate structures, and in acid media the carboxylic tautomer is favored. Furthermore upon reaction with acid some of the oxides come off as C0₂. This C0₂ chemisorbs on the carbon surface and generates stable carbonate structures.     

Cardiac Arrival Time Analyzer

The sequence of excitation of cardiac muscles is greatly affected by any abnormality in the conduction system of the heart. Epicardial mapping for the measurement of activation time at different points is helpful for the diagnosis of such abnormalities. For the measurement of activation time at different points of the epicardial surface, a number of sophisticated instruments, including microcomputer-based ones, have been reported. However, the need for a reliable, low-cost, and easy to operate instrument cannot be denied. In this note, such a cardiac arrival time analyzer is described.     

A very small dielectric planar filter for portable telephones

A high performance, high-dielectric stripline filter with the size of 4.5 mm×3.2 mm×2.0 mm has been developed. The filter consists of planar resonators and its structure is made of high permittivity multilayer ceramic in the Bi-Ca-Nb-O system. The performance is distinctive with its attenuation pole. An equivalent lumped circuit is derived to explain the behavior of the attenuation pole quantitatively. A precise design procedure is also described     

Activation of Rho-dependent cell spreading and focal adhesion biogenesis by the v-Crk adaptor protein

The small GTPase RhoA plays a critical role in signaling pathways activated by serum-derived factors, such as lysophosphatidic acid (LPA), including the formation of stress fibers in fibroblasts and neurite retraction and rounding of soma in neuronal cells. Previously, we have shown that ectopic expression of v-Crk, an SH2/SH3 domain-containing adapter proteins, in PC12 cells potentiates nerve growth factor (NGF)-induced neurite outgrowth and promotes the survival of cells when NGF is withdrawn. In the present study we show that, when cultured in 15% serum or lysophosphatidic acid-containing medium, the majority of v-Crk-expressing PC12 cells (v-CrkPC12 cells) display a flattened phenotype with broad lamellipodia and are refractory to NGF-induced neurite outgrowth unless serum is withdrawn. v-Crk-mediated cell flattening is inhibited by treatment of cells with C3 toxin or by mutation in the Crk SH2 or SH3 domain. Transient cotransfection of 293T cells with expression plasmids for p160ROCK (Rho-associated coiled-coil-containing kinase) and v-Crk, but not SH2 or SH3 mutants of v-Crk, results in hyperactivation of p160ROCK. Moreover, the level of phosphatidylinositol-4,5-bisphosphate is increased in v-CrkPC12 cells compared to the levels in mutant v-Crk-expressing cells or wild-type cells, consistent with PI(4)P5 kinase being a downstream target for Rho. Expression of v-Crk in PC12 cells does not result in activation of Rac- or Cdc42-dependent kinases PAK and S6 kinase, demonstrating specificity for Rho. In contrast to native PC12 cells, in which focal adhesions and actin stress fibers are not observed, immunohistochemical analysis of v-CrkPC12 cells reveals focal adhesion complexes which are formed at the periphery of the cell and are connected to actin cables. The formation of focal adhesions correlates with a concomitant upregulation in the expression of focal adhesion proteins FAK, paxillin, alpha3-integrin, and a higher-molecular-weight form of beta1-integrin. Our results indicate that v-Crk activates the Rho-signaling pathway and serves as a scaffolding protein during the assembly of focal adhesions in PC12 cells.