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Cardioprotective effects of a standardized extract from leaves with flowers of Crataegus (WS-1442; content of oligomeric procyandins [OPC]: 18.75%) have recently been demonstrated in an ischemia-reperfusion model in rats. Further studies were now conducted to clarify the mechanism of action and to identify active constituents involved in these effects of WS-1442. Exhausting partitioning between ethyl acetate/water and successive ultrafiltration of the aqueous layer led to the quantitative recovery of three fractions, which were tested for their in vitro radical scavenging (RS) and human neutrophil elastase (HNE) inhibitory activity. The lipophilic ethylacetate-soluble fraction A, enriched in flavone derivatives and constituting 14.9% of WS-1442, was as active as WS-1442 in inhibiting HNE. However, its RS activity was only about half that of the primary extract. Although 67.9% of WS-1442 was recovered in a water-soluble low molecular weight fraction B, this fraction displayed only weak RS and HNE inhibiting activity. In contrast, the RS and HNE inhibiting potencies of an essentially flavone-free and OPC-rich fraction C (21.3% of WS-1442) were significantly higher (inhibition of lipid peroxidation: IC50 0.3 microgram/ml; inhibition of HNE: IC50 0.84 microgram/ml) as those of WS-1442. The RS and HNE inhibitory activities of the extract and those of its fractions correlated well with their OPC-content but not with their concentration of flavonols. These results demonstrate that OPCs of Crataegus extracts possess stronger radical scavenging activities than flavone derivatives or other constituents. In addition, the oligomeric components are potent inhibitors of HNE. Oral administration of 20 mg/kg/d of the OPC-rich fraction C to rats afforded similar protection against ischemia-reperfusion induced pathologies as treatment with WS-1442 at a dose of 100 mg/kg/d. These observations indicate that radical scavenging and elastase inhibitory activities could indeed be involved in the observed cardioprotective effects of WS-1442, and demonstrate that OPCs are major orally active constituents of WS-1442. Thus, Crataegus extracts used therapeutically for cardiovascular diseases should be analyzed and standardized for their OPC-content. 相似文献
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The effectiveness of Cocos nucifera pollen extract immunotherapy (CPE-IT) was studied in 96 patients allergic to C. nucifera pollen. A placebo-controlled study was performed at random for a period of 6-12 months. The clinical status of the patients measured by the symptom-medication scores demonstrated that C. nucifera pollen-allergic patients had significant (p < 0.005) clinical improvement after CPE-IT in comparison to placebo treatment. Serological study resulted a significant reduction (p < 0.001) of specific IgE and significant elevation (p < 0.01) of specific IgG in post-therapeutic patients' sera which were correlated significantly (r = 0.45, p < 0.001); the changes of the above immunoglobulin levels in the placebo-treated patients were nonsignificant. However, there was no correlation between symptom-medication scores and changes in specific serum IgE or IgG levels. 相似文献
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We present a hybrid representation for the attachment-mode current existing on the surface of a coaxially fed rectangular microstrip patch antenna. The hybrid representation consists of a one- or two-term residue and an eigenfunction series which gives the attachment-mode current at all points on the patch surface, including the probe-to-patch junction. It is numerically demonstrated that the residue and eigenfunction series blend smoothly in the close vicinity of the Stokes region which includes the probe-to-patch junction. The Stokes region is a very narrow region over which the attachment-mode current changes rapidly. The residue and eigenfunction series are used outside and within this Stokes region, respectively. This hybrid representation can be used in either spectral- or space-domain techniques for a full-wave solution to the microstrip antenna problem. The residue series is obtained by using an equivalent contour integral representation of the infinite eigenfunction series, which subsequently reduces to the Watson transform under the assumption of a slightly lossy substrate. At or near the Stokes regions the residue series is inadequate, as it cannot model the rapidly varying junction currents there. Examination of the residue series shows that the attachment mode is a superposition of exponentially attenuated, leaky, surface-traveling modes. This hybrid representation is expected to provide the optimum trade-off between speed and accuracy for computations involving electrically large finite arrays 相似文献
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Sample preparation for DNA and RNA assays is a prime candidate for laboratory automation. A novel, parallel processing device that performs the three separate liquid-handling functions necessary for such sample preparation-dispensing, pipetting, and pressurizing-is presented. The device comprises an array of fine nozzles connected by fluidic channels to automatically and precisely distribute flow between one source and an array of points. The design principles, as well as the experimental and computational methods used to develop the device, are described. Test results, including accuracy, uniformity, volume range, and timing, are presented. The functionality of the device is demonstrated by performing a solid-phase extraction of DNA with two types of microcolumns. 相似文献
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The addition of ammonium chloride, cobalt sulphate, cesium sulphate and ethylene diamine to a Mattsson solution and the addition of ammonium sulphate to the Pugh solution have been found to produce an inhibitive effect on the stress corrosion cracking of alpha-brass in these solutions. Ammonium chloride and ammonium sulphate additions affect the tarnish formation on the specimen as well as the cracking pattern. Electrochemical polarization studies have also been carried out with these two additives. The observations have been explained in the light of adsorption of these ionic species at selected sites on the metal surface and their possible interference with the cathodic reactions. 相似文献
9.
When a jet of soluble gas is directed on to liquid surface, the kinetic energy of the jet is transferred to the liquid giving rise to circulation, which in turn enhances mass transfer. Consequently, the mass transfer coefficient is related to the characteristics of the jet. 相似文献
10.
Chatterjee Abhishek Ghaednia Hamid Bowling Alan Brake Matthew 《Multibody System Dynamics》2021,51(1):45-90
Multibody System Dynamics - Collision between hard objects causes abrupt changes in the velocities of the system, which are characterized by very large contact forces over very small time... 相似文献