水稻基因组MSAP指纹图谱构建及DNA甲基化修饰位点分离与鉴定

采用EcoR I和Hpa II/Msp I双酶切建立了适合于水稻基因组的甲基化敏感扩增多态性(MSAP)分析体系,在全基因组水平检测了水稻DNA甲基化修饰位点.以12对MSAP引物进行选择性扩增,共检测到甲基化修饰位点120个,"CCGG/GGCC"位点甲基化修饰比例为20.17%.对部分水稻基因组甲基化修饰位点进行回收,最终分离了55条存在甲基化位点变异的DNA序列,通过BLAST比对分析将其联配到水稻基因组序列上.分析表明,这些甲基化修饰位点主要集中于基因启动子区(47%)和第一外显子区(22%),在其侧翼序列中存在类似"CpG island" 典型序列特征的"CpG"二核苷酸成簇富集区.在此基础上,对应用MSAP技术分离水稻基因组DNA甲基化修饰位点的有效性以及水稻基因组序列中"CpG island"类似序列分布特征和生物意义进行了讨论.     

Critical Roles of Circular RNA in Tumor Metastasis via Acting as a Sponge of miRNA/isomiR

Circular RNAs (circRNAs), a class of new endogenous non-coding RNAs (ncRNAs), are closely related to the carcinogenic process and play a critical role in tumor metastasis. CircRNAs can lay the foundation for tumor metastasis via promoting tumor angiogenesis, make tumor cells gain the ability of migration and invasion by regulating epithelial-mesenchymal transition (EMT), interact with immune cells, cytokines, chemokines, and other non-cellular components in the tumor microenvironment, damage the normal immune function or escape the immunosuppressive network, and further promote cell survival and metastasis. Herein, based on the characteristics and biological functions of circRNA, we elaborated on the effect of circRNA via circRNA-associated competing endogenous RNA (ceRNA) network by acting as miRNA/isomiR sponges on tumor angiogenesis, cancer cell migration and invasion, and interaction with the tumor microenvironment (TME), then explored the potential interactions across different RNAs, and finally discussed the potential clinical value and application as a promising biomarker. These results provide a theoretical basis for the further application of metastasis-related circRNAs in cancer treatment. In summary, we briefly summarize the diverse roles of a circRNA-associated ceRNA network in cancer metastasis and the potential clinical application, especially the interaction of circRNA and miRNA/isomiR, which may complicate the RNA regulatory network and which will contribute to a novel insight into circRNA in the future.     

The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, GmPUB21, a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of GmPUB21, which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the Nicotiana benthamiana over-expressing GmPUB21 showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in GmPUB21 overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of GmPUB21 in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that GmPUB21 negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of GmPUB21 under drought and salinity stresses in soybean.     

Velocity and turbulence characteristics in an open channel with multi-row staggered vegetation patches

A staggered distribution of vegetation is very common in nature,which might significantly affect the flow structure.To investigate its impact,three rows of stag...     

浅析建立企业标准体系必须结合企业自身实际情况

目前，很多企业已经建立了GB／T19001、GB／T28001、GB／T24001管理体系，为了在更大的范围和更深的层次上提高企业的管理水平，一些企业还在寻求更多管理体系的认证或确认，还有些企业在寻求不同管理体系的整合和提高，按照GB／T15496、GB／T15497、GB／T15498和GB／T19273的2003版标准建立的企业标准体系也是规范企业所有的管理工作的有效手段。对于有管理经验积累的企业而言，管理体系的运行在一定时期后，企业标准化水平会得到一定提高。但是，如何不断提高运行的有效性与更好地协调各管理体系的兼容性，是摆在我们面前的一个新课题。笔者结合本公司建立企业标准体系的实践经验，阐述了建立企业标准体系，实现企业管理水平持续改进必须要结合企业自身实际情况。     

基于LCC理论的软件项目多目标权衡

传统的软件项目成本模型只反映出与工作量有关的费用,对于因质量不足而引起的成本无法度量.将全寿命周期成本(LCC)理论应用于软件项目成本计算,按照分部分项方法建立了软件项目的完全成本模型.在软件项目工期-成本二维权衡模型的基础上,通过设置多级指标体系对软件项目质量进行量化,从而将软件质量纳入权衡目标,在此基础上构建了软件项目工期、成本、质量的多目标权衡离散模型,运用改进的禁忌搜索算法求解模型,以可视化三维曲面输出帕累托非劣解,最后分析了项目决策者获得项目最佳实施模式的途径.     

The working mechanism of organic photovoltaic cell by using copper phthalocyanine as exciton blocking layer

We demonstrate the working mechanism of organic photovoltaic (OPV) cell with copper phthalocyanine (CuPc) as exciton blocking layer (EBL). The new EBL material CuPc, commonly has been used as electron donor in the organic solar cells due to its electron-donating and hole-transporting properties. But here we proves that the α-polymorph CuPc layer can transfer electrons to Al cathode through the half-filled b_1g level, this mechanism is different from that of general EBL material with larger band gap and electron-transporting property, which is based on damage states induced by the heat of evaporating Al.     

牛α-乳白蛋白-油酸复合物对肿瘤细胞能量代谢的影响

本研究以人肝癌细胞(HepG2)为对象,分为α-LA-OA处理组、牛-α乳白蛋白(α-LA)处理组和溶剂对照组,以细胞的糖酵解和线粒体有氧呼吸2个过程中胞外酸化率和细胞耗氧率的变化水平为衡量指标,评估α-LA-OA对肿瘤细胞能量代谢的影响.结果表明,α-LA-OA能够显著性降低人肝癌细胞的糖酵解能力以及线粒体有氧呼吸能...     

Strength,strain capacity and toughness of five dual-phase pipeline steels

The effect of microstructures on strength, strain capacity and low temperature toughness of a micro-alloyed pipeline steel was elucidated. Five various dual-pha...