A procedure for measuring peptides in rumen fluid and evidence that peptide uptake can be a rate-limiting step in ruminal protein degradation

A relatively simple and sensitive procedure was developed to measure the concentration of peptides in rumen fluid. Feed particles and microorganisms were removed by centrifugation, and the supernatant was treated with perchloric acid (5% final concentration). Perchloric acid precipitated macromolecules that included protein, RNA, and DNA. Perchlorate was subsequently removed by precipitation with an excess of potassium carbonate. Ammonia was removed by boiling the alkaline sample. Supernatant samples were then analyzed for ninhydrin reactive material before and after HCl hydrolysis. Because ninhydrin reaction was 3 to 7.5 times greater after HCl hydrolysis, peptides rather than amino acids were the primary source of nonprotein, nonammonia nitrogen. Rumen fluid from a cow fed timothy hay and concentrate supplement (16% crude protein) contained more than 1200 mg peptides/L (192 mg N/L), 1 h after feeding, and this value declined the prefeeding value of 400 mg/L (64 mg N/L) by 8 h after feeding. Comparison of ninhydrin reactivity with and without HCl hydrolysis indicated that peptides present before feeding contained more peptide bonds than the peptides soon after feeding. High pressure liquid chromatography revealed a variety of peaks soon after feeding and fewer dominant peaks 8 h later. The data suggest that peptide uptake into rumen microorganisms can be a rate-limiting step in ruminal protein degradation.     

Kinetics of Folacin Destruction in Swiss Chard During Storage

The folacin activities in fresh Swiss chard leaves stored in open air at 4, 21, 35 and 40°C were determined by a microbiological assay using Lactobacillus casei. At 21°C the leaves were also stored in plastic bags and under moist conditions. Folacin was most stable when the vegetable was stored in plastic bags, followed by the moist condition, and least stable in open air at 21°C. The degradation of folacin in Swiss chard under all conditions followed firstorder kinetics. The temperature dependent folate degradation conformed to the Arrhenius equation and the activation energy was 24 kcal/mole.     

Separating inviscid flows

Summary The separating flow of an inviscid fluid is not only a limit solution of the steady separating, laminar fluid flow at high values of the Reynolds number but it is also part of its structure (Smith [1], [2]). This work aims at reexamining the separating flow of inviscid fluid past a bluff body which is fixed in an otherwise uniform stream of fluid. For the purpose of this paper we will assume that the bluff body is a circular cylinder but the theory is applicable to bodies of any shape. It is further assumed that the fluid is in steady two-dimensional motion and is inviscid and of constant density. The flow structure is assumed to consist of a separated flow region, caviting flows in which there exists a free surface on which the pressure is constant, and a wake. A twin spiral vortex model is used in order to determine the shape of the free streamline. Based on the free streamline theory the problem reduces to solving a mixed boundary value problem and a Hilbert solution for the inverse problem in the auxiliary plane is obtained. When we consider the flow in the physical plane the problem is transformed into a direct problem in which the geometry of the solid body is given in advance. We assume that the separation is smooth and thus the curvature of the free streamline at the point of free detachment be equal to that of the body surface. A numerical method for solving the two-dimensional potential flows past arbitrarily shaped curved bluff bodies is developed.When the cavitation number is zero the angle of separation is approximately 55° and the computed results predict that the position of the separation point will move backward as the cavition number increases. The relationships between the drag coefficient, and the width and length of the cavity is determined and is found to be in very good agreement with the predictions of Smith [1].     

Transition From Perturbed to Coupled-Cavity Behavior With Asymmetric Spectral Emission in Ridge Lasers Emitting at 1.55 μm

We have investigated a series of ridge waveguide lasers with deeply etched slots in the ridges. The slots do not penetrate the active region, but are deep enough to strongly perturb the longitudinal modes. By the addition of slots, a transition between perturbed-mode and coupled-cavity behavior is crossed. With a group of four or more slots, the below-threshold amplified spontaneous emission spectrum from each end of the laser has different periods and the facet-facet oscillations are suppressed indicating that the different sections have become quasi-independent. A model using a distributed emitter in the cavity reproduces this behavior. Above threshold, the single contact coupled cavity lasers are single mode with greater than 30-dB sidemode suppression ratio over a wide range of currents     

Surface tension isotherms of a free surface and grain boundaries in the Cu-Sn system

Surface tension of pure copper and its alloys with Sn has been measured at 1000°C in a hydrogen atmosphere. Surface tension of a free surface equaled 1.81 N/m for pure copper, and 1.55, 1.38, and 1.28 N/m for the alloys with 0.05, 0.22, and 0.33 at % Sn, respectively; surface tension at grain boundaries equaled 0.5 N/m for pure copper, and 0.36, 0.3, and 0.23 N/m for the alloys, respectively. Based on these data, the concentration dependence of tin absorption at a free surface and grain boundaries has been calculated. Parameters of Sn absorption at grain boundaries and a free surface of copper have been compared with those of Sb and Bi.     

Emergy account for biomass resource exploitation by agriculture in China

On the basis of Odum's ecological economic measure of emergy as embodied solar energy, a system account of biomass resource exploitation by agriculture in China 2004 is developed in this paper, which supplements a former study on corresponding long-term historical trends during 1978 to 2000 (Chen et al., 2006. Emergy-based analysis of the Chinese agriculture. Agriculture, Ecosystems and Environment 115, 161–173). The aggregate fluxes and indicators for biomass resource exploitation in China 2004 are calculated and illustrated when compared with those for 2000 to elucidate the latest status of the Chinese agriculture as the exploitation sector for biomass resource. Data sources and algorithm are presented in detail as basic references for related analysis involving the ecological economy of biomass exploitation in agriculture.     

A DSP-Based Remote Control Laboratory

This paper presents a framework for rapid remote experiment implementation in the field of automatic control. The proposed solution is based on in-house developed embedded control hardware and two commercially available software packages. MATLAB/Simulink is used for rapid experiment control algorithm development, while LabVIEW is used for the user front-end and remote control. A combination of presented hardware and software solutions enables the rapid and easy creation of different interactive remote control experiments. Using this solution, a digital-signal-processor-based remote control laboratory for teaching purposes has been realized. This remote laboratory enables the remote users to easily interact with a set of physical control experiments through the Internet. In the friendly user interface, the remote user can change predefined system parameters and observe system response in textual, graphical, or video format. In addition, this remote laboratory includes a booking system, which enables remote users to book experiments in advance.     

A polydispersed particle system representation of the porosity for non-saturated cementitious materials

In this paper, the porosity of cementitious materials is described in terms of pore size distribution by means of a 3-dimensional overlapping sphere system with polydispersivity in size. On the basis of results established by Lu and Torquato [B. Lu, S. Torquato, Nearest-surface distribution functions for polydispersed particle systems, Phys. Rev. A 45(8) (1992) 5530-5544] and Torquato [S. Torquato, Random Heterogeneous Media: Microstructure and Macroscopic Properties. Springer-Verlag: New York, 2001] providing relations for nearest-neighbor distribution functions, the volume fraction of pores having a radius larger than a prescribed value is explicitly expressed. By adopting an appropriate size distribution function for the sphere system, it is shown that the pore size distribution of cementitious materials as detected for instance by mercury intrusion porosimetry (MIP), which generally points out several pore classes, can be well approached. On the basis of this porosity representation, the evaluation of the capillary pressure in function of the saturation degree is provided. The model is then applied to the simulation of the saturation degree versus relative humidity adsorption curves. The impact of the pore size distribution, the temperature and the thickness of the adsorbed water layer on these parameters are assessed and analyzed for three model materials having different pore characteristics.     

Impedance spectroscopy of ultrafine-grain SnO2 ceramics with a variable grain size

The impedance spectra of nanocrystalline SnO₂ ceramics with an average grain size d ranging from 3 to 43 nm were investigated in the frequency range 1–10⁶ Hz at temperatures from 25 to 300°C in a dry oxygen atmosphere. Analysis of the experimental data by the graphoanalytical method made it possible to separate the contributions of grain bulk and grain boundaries to the conductivity. It is shown that the samples investigated can be arbitrarily divided into two groups with respect to the character of their conductivity. For the samples with an average grain size d < 25 nm, the charge transfer processes are almost completely determined by the grain boundaries. In samples with a larger grain size, the contribution of grain bulk to the conductivity is comparable with that of grain boundaries.