Uricase-immobilization on radiation grafted polymer support for detection of uric acid using Ag-nanoparticle based optical biosensor

A functional polymer support was designed for covalent immobilization of enzyme uricase, which was further utilized in a single enzyme based silver nanoparticles (Ag-NPs)-optical biosensor system for estimation of uric acid. A carboxylic acid functionalized polymer support was fabricated via gamma radiation induced mutual-irradiation grafting of polyacrylic acid (PAA) on to non-woven polypropylene (PP) matrix. Grafting parameters, such as dose, monomer concentration, homopolymer inhibitor and inorganic acid concentration were studied to optimize the grafting process. PAA-g-PP samples were characterized by grafting yield measurement, water uptake, FTIR, TGA and SEM techniques. Uricase enzyme was immobilized on to the PAA-g-PP support via covalent amide linkage using coupling agents, namely N-hydroxysuccinimide (NHS) and N-ethyl-N′-(3-(dimethylamino)propyl)carbodiimide (EDC). The catalytic activity of uricase was investigated spectrophotometrically using uric acid as a substrate. Study of kinetic parameters (K_m, V_max) revealed the value of K_m for covalently immobilized uricase to be ∼1.2-fold higher than that of free enzyme. The uricase-immobilized-PAA-g-PP samples could be repeatedly used for ∼20 cycles over a period of 30 days when stored in phosphate buffer (pH∼7.4) at 4°C without significant decrease in the enzyme activity. The uricase immobilized samples were successfully used in conjunction with the radiolytically synthesized Ag-NPs as a LSPR-optical biosensor for estimation of uric acid.     

External stimulus-responsive biomaterials designed for the culture and differentiation of ES,iPS, and adult stem cells

The physical and chemical characteristics of biomaterial surface and hydrogels can be altered by external stimuli, such as light irradiation, temperature changes, pH shifts, shear stress forces, electrical forces, and the addition of small chemical molecules. Such external stimulus-responsive biomaterials represent promising candidates that have been developed for the culture and differentiation of embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, and adult stem cells. Biomaterials that are designed to respond in a reversible manner to specific external signals can be formed on micropatterned or non-micropatterned surface, in hydrogels, or on microcarriers. Stem cells and the cells differentiated from them into specific tissue lineages can be cultured and/or differentiated on dishes with immobilized external stimulus-responsive polymers. Cells can be detached from these dishes without using an enzymatic digestion method or a mechanical method when the appropriate external stimulus is generated on the surface. This review discusses the polymers and polymeric designs employed to produce surface and hydrogels for stem cell culture, differentiation, and/or cell detachment using various external stimuli.     

Solid‐state NMR study of spin finish of thermally treated PAN and PAN/CNT precursor fibers

Dry‐jet wet‐spun polyacrylonitrile (PAN) and PAN/carbon nanotubes (CNTs) precursor fibers coated by spin finishes were characterized using the solid‐state ¹H nuclear magnetic resonance technique. Series of fiber samples were prepared upon thermal treatment at different temperatures (room temperature to 180°C). Using the Hahn echo sequence, relatively mobile components were identified and the effect of the heat treatment on those components was studied. It was observed that the mobile components are mainly the spin finishes. Heat treatment caused loss of one of the spin finishes (Type B) to a great extent (～80%), whereas the other two spin finishes (Type A and Type C) were more stable. Additional information regarding the change in molecular mobility due to heat treatment was obtained by the spin‐lattice relaxation time ( T₁ ) analysis. It was found that the presence of CNT affects the T₁ relaxation time of the polymer in the composite fiber, however, that of relatively mobile components remains unaffected. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40734.     

Gelatin‐carbohydrate phase‐separated hydrogels as bioactive carriers in vaginal delivery: Preparation and physical characterizations

The current study focuses on the alteration of properties of the gelatin hydrogels using polysaccharides (e.g., maltodextrin, dextran, and sodium carboxymethyl cellulose) for probable use in vaginal delivery of antimicrobials. The hydrogels were prepared by varying the proportions of gelatin and polysaccharides and were characterized by microscopy, mechanical testing, and impedance spectroscopy. Metronidazole (MZ), drug of choice for the treatment of bacterial vaginosis, was incorporated within the hydrogels. In vitro release studies of MZ from the hydrogels was studied in‐depth using modified Franz's diffusion cell. Antimicrobial efficiency of the MZ‐loaded hydrogels was tested against E. coli and B. subtilis. The results suggested that the incorporation of polysaccharides resulted in the phase‐separated hydrogels. The properties of the hydrogels was found be suitable for vaginal delivery. The drug release and antimicrobial efficiency from the hydrogels suggested that the developed hydrogels may be used for the delivery of antimicrobials in the vaginal lumen. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40445.     

Mechanical and thermal characterization of compression moulded polylactic acid natural fiber composites reinforced with hemp and lyocell fibers

This research evaluates the effects of PLA/PP blend ratio and Lyocell/hemp mixture ratio on the morphology, water absorption, mechanical and thermal properties of PLA‐based composites. The composites were fabricated with 30 mass % hemp using compression moulding. As a reference composites made from PP were also studied. Combining of hemp and Lyocell in PLA composite leads to the reduction of moisture absorption and can improve the impact, tensile, flexural properties when compared with PLA/hemp. Composite based on the PLA/PP blend‐matrix could not improve the tensile and flexural properties compared with PLA/hemp, however; the lighter composite with better impact properties was obtained. The crystallization temperature of the PLA‐PP/hemp increased compared with pure PLA. This result was also confirmed by the SEM micrographs. The moisture absorption of PLA‐PP/hemp was higher than PLA/hemp. Based on theoretical analysis of DMTA data, there was favorable adhesion in all composites. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40534.     

Synthesis of graphene/vitamin C template-controlled polyaniline nanotubes composite for high performance supercapacitor electrode

A graphene nanosheet/polyaniline nanotube (GPNT) composite is prepared for the first time by in-situ chemical oxidative polymerization of aniline using vitamin C as a structure directing agent. The vitamin C molecules lead to the synthesis of polyaniline (PANI) nanotubes through the development of rod-like assembly by H-bonding in an aqueous medium. The initially synthesized graphene oxide/polyaniline nanotubes composite is reduced to graphene using hydrazine monohydrate followed by re-oxidation and protonation of the PANI to produce the GPNT nanocomposite. This novel composite showed a high specific capacitance of 534.37 F/g and an excellent energy density of 74.27 Wh/kg at a constant current of 0.5 mA. Besides, the GPNT composite exhibited excellent cycle life with 91.4% specific capacitance retained after 500 charge-discharge cycles. The excellent performance is due to the synergistic combination of graphene which provides good electrical conductivity and mechanical stability, and PANI nanofiber which deals with good redox activity.     

Development of a gel spinning process for high‐strength poly(ethylene oxide) fibers

This article describes a new gel‐spinning process for making high‐strength poly(ethylene oxide) (PEO) fibers. The PEO gel‐spinning process was enabled through an oligomer/polymer blend in place of conventional organic solvents, and the gelation and solvent‐like properties were investigated. A 92/8 wt% poly(ethylene glycol)/PEO gel exhibited a melting temperature around 45°C and was highly stretchable at room temperature. Some salient features of a gel‐spun PEO fiber with a draw ratio of 60 are tensile strength at break = 0.66 ± 0.04 GPa, Young's modulus = 4.3 ± 0.1 GPa, and a toughness corresponding to 117 MJ/m³. These numbers are significantly higher than those previously reported. Wide‐angle x‐ray diffraction of the high‐strength fibers showed good molecular orientation along the fiber direction. The results also demonstrate the potential of further improvement of mechanical properties. POLYM. ENG. SCI., 54:2839–2847, 2014. © 2014 Society of Plastics Engineers     

Dendrimer as nanocarrier for drug delivery

Dendrimers are novel three dimensional, hyperbranched globular nanopolymeric architectures. Attractive features like nanoscopic size, narrow polydispersity index, excellent control over molecular structure, availability of multiple functional groups at the periphery and cavities in the interior distinguish them amongst the available polymers. Applications of dendrimers in a large variety of fields have been explored. Drug delivery scientists are especially enthusiastic about possible utility of dendrimers as drug delivery tool. Terminal functionalities provide a platform for conjugation of the drug and targeting moieties. In addition, these peripheral functional groups can be employed to tailor-make the properties of dendrimers, enhancing their versatility. The present review highlights the contribution of dendrimers in the field of nanotechnology with intent to aid the researchers in exploring dendrimers in the field of drug delivery.     

Development of topical gel containing aceclofenac-crospovidone solid dispersion by “Quality by Design (QbD)” approach

This article describes the development, optimization, and evaluation of Carbopol 940 topical gel containing aceclofenac-crospovidone (1:4) solid dispersion using “Quality by Design (QbD)” approach based on 2³ factorial design. The effect of crospovidone, tri-ethanolamine, and ethyl alcohol amount on the drug permeation profile of the topical gel containing aceclofenac-crospovidone solid dispersion was optimized by 2³ factorial design. The optimized gel showed improved permeation profile with cumulative drug permeation of 26.262 ± 2.157%, and permeation flux of 0.059 ± 0.011 μg/cm²/h. These gels were characterized by pH, viscosity, gel strength and FTIR study. The in vivo anti-inflammatory activity of the optimized gel was evaluated in rats using carrageenan-induced rat-paw oedema model and found excellent anti-inflammatory comparable with a marketed gel without producing any skin irritation.     

Recovery of copper from a surface altered chalcopyrite contained ball mill spillage through bio-hydrometallurgical route

Bioleaching studies for chalcopyrite contained ball mill spillages are very scarce in the literature. We developed a process flow sheet for the recovery of copper metal from surface activated (600 °C, 15 min) ball mill spillage through bio-hydrometallurgical processing route. Bioleaching of the activated sample using a mixed meso-acidophilic bacterial consortium predominantly A. ferrooxidans strains was found to be effective at a lixiviant flow rate of 1.5 L/h, enabling a maximum 72.36% copper recovery in 20 days. Mineralogical as well as morphological changes over the sample surface were seen to trigger the bioleaching efficiency of meso-acidophiles, thereby contributing towards an enhanced copper recovery from the ball mill spillage. The bio-leach liquor containing 1.84 g/L Cu was purified through solvent extraction using LIX 84I in kerosene prior to the recovery of copper metal by electrowinning. Purity of the copper produced through this process was 99.99%.