Two-dimensional multiarray formation of hepatocyte spheroids on a microfabricated PEG-brush surface

A two-dimensional microarray of ten thousand (100 x 100) hepatocyte heterospheroids, underlaid with endothelial cells, was successfully constructed with 100 microm spacing in an active area of 20 x 20 mm on microfabricated glass substrates that were coated with poly(ethylene glycol) brushes. Cocultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100 microm, functioning as a miniaturized liver to secret albumin for at least one month. The most important feature of this study is that these substrates are defined to provide an unprecedented control of substrate properties for modulating cell behavior, employing both surface engineering and synthetic polymer chemistry. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors and detects a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.     

Estimating aboveground biomass of a mangrove plantation on the Northern coast of Vietnam using machine learning techniques with an integration of ALOS-2 PALSAR-2 and Sentinel-2A data

ABSTRACT

Aboveground biomass (AGB) of mangrove forest plays a crucial role in global carbon cycle by reducing greenhouse gas emissions and mitigating climate change impacts. Monitoring mangrove forests biomass accurately still remains challenging compared to other forest ecosystems. We investigated the usability of machine learning techniques for the estimation of AGB of mangrove plantation at a coastal area of Hai Phong city (Vietnam). The study employed a GIS database and support vector regression (SVR) to build and verify a model of AGB, drawing upon data from a survey in 25 sampling plots and an integration of Advanced Land Observing Satellite-2 Phased Array Type L-band Synthetic Aperture Radar-2 (ALOS-2 PALSAR-2) dual-polarization horizontal transmitting and horizontal receiving (HH) and horizontal transmitting and vertical receiving (HV) and Sentinel-2A multispectral data. The performance of the model was assessed using root mean square error (RMSE), mean absolute error (MAE), coefficient of determination (R²), and leave-one-out cross-validation. Usability of the SVR model was assessed by comparing with four state-of-the-art machine learning techniques, i.e. radial basis function neural networks, multi-layer perceptron neural networks, Gaussian process, and random forest. The SVR model shows a satisfactory result (R² = 0.596, RMSE = 0.187, MAE = 0.123) and outperforms the four machine learning models. The SVR model-estimated AGB ranged between 36.22 and 230.14 Mg ha^?1 (average = 87.67 Mg ha^?1). We conclude that an integration of ALOS-2 PALSAR-2 and Sentinel-2A data used with SVR model can improve the AGB accuracy estimation of mangrove plantations in tropical areas.     

Preparation and evaluation of gel formulations for oral sustained delivery to dysphagic patients

Background: Oral sustained release gel formulations may provide a means of administering drugs to dysphagic and geriatric patients who have difficulties with handling and taking oral dosage forms. Aim: We have designed gel formulations for the oral administration of paracetamol with suitable rheological characteristics for ease of administration to patients with swallowing difficulties and sufficient integrity in the acidic environment of the stomach to achieve a sustained release of this drug. Method: Gels formed by gelatin, agar, gellan, pectin, and xyloglucan were assessed for suitable gel strength and in vitro and in vivo release characteristics. Results: Gellan (1.5%?w/v) and xyloglucan gels (1.5%?w/w) had acceptable gel strengths for ease of swallowing and retained their integrity in the rat stomach sufficiently well to sustain the release of paracetamol over a period of 6 hours. Comparison of 1.5%?xyloglucan gels with a commercially available preparation with identical paracetamol concentrations demonstrated improved sustained release properties of the xyloglucan gels. Conclusions: Gels formed by gellan and xyloglucan have suitable rheological and sustained release characteristics for potential use as vehicles for oral delivery of drugs to dysphagic patients.     

High-velocity impact experiment of aluminum foam sample using powder gun

Porous materials such as aluminum foam have been investigated for possible use as impact shock absorbers in transportation aeronautic applications. However, the response of aluminum foam during impacts at high velocities of more than 100 m/s is not yet fully understood. A high-velocity impact experiment was therefore carried out to clarify impact shock absorption properties of aluminum foam. A one-stage powder gun was used to accelerate an aluminum foam sample to impact a rigid wall. Velocity and deformation of the aluminum foam sample during impact was studied using a digital high-speed video camera, while the pressure wave in the aluminum foam sample was measured using a PVDF gauge. The experimental observations revealed uneven collapse of the aluminum foam sample structure during high speed impact with a general stress plateau effect, typical for cellular material structures when subjected to quasi-static loading.     

Oral Sustained Delivery of Theophylline and Cimetidine from In Situ Gelling Pectin Formulations in Rabbits

ABSTRACT

The aim of this study was to evaluate the potential of an in situ gelling pectin formulation as a vehicle for the oral sustained delivery of theophylline and cimetidine. In vitro studies demonstrated diffusion-controlled release of theophylline from 1, 1.5, and 2% w/v pectin gels. Release of this drug from 1.5% w/v pectin gels formed in situ in rabbit stomach was sustained over a period of 12 hours giving a theophylline bioavailability some seven fold higher than when administered from a commercial syrup. In contrast, interactions between cimetidine and pectin led to weak gelation of the pectin sols that prevented any meaningful determination of in vitro release characteristics. Similarly, in vivo release profiles from pectin formulations containing cimetidine were similar to that from a solution of this drug in buffer, indicative of weak gelation. Examination of the content of the rabbit stomach 5 hours after administration of 1.5% w/v pectin sols containing drug confirmed gel formation, but gels containing cimetidine were noticeably softer than those containing theophylline.     

XPS analysis of CdS/CuInSe2 heterojunctions

CdS/CuInSe₂ (CIS) heterojunctions were investigated by XPS analysis. An In-excess layer which may form an ordered vacancy compound (OVC) was present at the as-deposited CIS surface and it remained after chemical bath deposition of a CdS layer. The In-excess layer was removed by preferential etching with NH₃ aqueous solution. This result implies that the surface of the as-deposited CIS film was converted from the OVC with n-type conductivity into the CIS with p-type by NH₃ treatment. The conduction band offsets at the CdS/p-CIS and CdS/n-OVC were determined to be 1.0 and 0.3 eV, respectively. The CIS solar cells fabricated with n-OVC surface layer exhibited higher cell efficiencies than those fabricated with p-CIS surface layer.     

L-Ergothioneine scavenges superoxide and singlet oxygen and suppresses TNF-α and MMP-1 expression in UV-irradiated human dermal fibroblasts

The hair lipid composition collected from 44 Japanese females between 1 and 81 years of age was examined for eight lipids including hydrocarbons (HCs), squalene (SQ), wax esters (WEs), triglycerides (TGs), fatty acids (FAs), cholesterol (CH), ceramides (CERs), and 18-methyl eicosanoic acid (MEA). In this study, the 5-cm length from the proximal root end of hair fibers, which had never been exposed to any chemical treatment, was used after 5-min incubation with hexane following shampooing. Hair lipids were extracted with solvent and subsequent alkali-solvent and were then analyzed by a combination of chromatography. Although the average contents of the lipids showed great fluctuations among individuals, there were significant correlations between the levels of each lipid, which allowed for the classification of the hair lipids into four groups: group A: SQ, WEs, TGs, and FAs (designated as endogenous lipids based upon their sebum origin); group B: CH and CERs (designated as endogenous lipids); group C: HC (unknown origin); and group D: MEA (the other endogenous lipid). A principal component analysis for eight lipids revealed that the hair lipid composition was characterized by a predominant negative correlation between each lipid for groups A and B. This negative correlation suggests that the endogenous lipids in group B serve as a barrier against the penetration of predominantly sebum-derived exogenous lipids (group A). Endogenous lipids consisting of CH and CERs (group B) and MEA (group D) should be designated as intrinsic internal lipids of human hair.     

Development of a new loading test apparatus for microfocus X-ray CT and its application to the investigation of soil behavior surrounding driven open-section piles

In recent years, X-ray CT scanners with task-specific modifications, such as microfocus and/or a synchrotron, have been widely implemented in a variety of fields, including medical and industrial fields. Since microfocus X-ray CT systems enable the visualization of whole samples, and also yield a relatively high resolution of the region of interest in the sample, they are commonly utilized in the fields of soil mechanics and geotechnical engineering. This paper describes a novel loading test apparatus that is specifically designed for microfocus X-ray CT systems capable of performing loading tests to investigate soil behavior during the installation of driven open-section piles. The loading tests were designed to take the micro-level spatial resolution of microfocus X-ray CT systems into account. Digital image correlation (DIC) is subsequently implemented to analyze the obtained CT images, making it possible for measurements to be taken of the displacement fields in the ground following installation of the model pile. Finally, the versatility of this novel test apparatus is proof of its high potential for application in different types of loading tests.