全文获取类型
收费全文 | 5001篇 |
免费 | 353篇 |
国内免费 | 7篇 |
专业分类
电工技术 | 29篇 |
综合类 | 6篇 |
化学工业 | 1716篇 |
金属工艺 | 41篇 |
机械仪表 | 78篇 |
建筑科学 | 193篇 |
矿业工程 | 5篇 |
能源动力 | 103篇 |
轻工业 | 926篇 |
水利工程 | 44篇 |
石油天然气 | 17篇 |
无线电 | 229篇 |
一般工业技术 | 631篇 |
冶金工业 | 757篇 |
原子能技术 | 33篇 |
自动化技术 | 553篇 |
出版年
2024年 | 10篇 |
2023年 | 71篇 |
2022年 | 390篇 |
2021年 | 386篇 |
2020年 | 175篇 |
2019年 | 161篇 |
2018年 | 171篇 |
2017年 | 157篇 |
2016年 | 173篇 |
2015年 | 167篇 |
2014年 | 215篇 |
2013年 | 308篇 |
2012年 | 282篇 |
2011年 | 350篇 |
2010年 | 253篇 |
2009年 | 255篇 |
2008年 | 259篇 |
2007年 | 243篇 |
2006年 | 173篇 |
2005年 | 138篇 |
2004年 | 139篇 |
2003年 | 122篇 |
2002年 | 104篇 |
2001年 | 41篇 |
2000年 | 55篇 |
1999年 | 48篇 |
1998年 | 60篇 |
1997年 | 40篇 |
1996年 | 46篇 |
1995年 | 37篇 |
1994年 | 38篇 |
1993年 | 33篇 |
1992年 | 21篇 |
1991年 | 10篇 |
1990年 | 21篇 |
1989年 | 22篇 |
1988年 | 25篇 |
1987年 | 22篇 |
1986年 | 19篇 |
1985年 | 20篇 |
1984年 | 10篇 |
1983年 | 11篇 |
1982年 | 14篇 |
1981年 | 11篇 |
1980年 | 4篇 |
1979年 | 12篇 |
1978年 | 9篇 |
1976年 | 4篇 |
1975年 | 3篇 |
1973年 | 4篇 |
排序方式: 共有5361条查询结果,搜索用时 15 毫秒
51.
Coping with a serious and chronic illness poses unique challenges for individuals in helping professions. What techniques and resources can be used to help the helpers? A narrative framework using the process of externalizing the problem is presented as a potentially useful approach for psychotherapists experiencing a serious illness. The author describes her own experience with lupus and offers recommendations for therapists struggling to balance their professional responsibilities with their own personal needs. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
52.
Kehoe Priscilla; Shoemaker William J.; Triano Laura; Callahan Megan; Rappolt Gabrielle 《Canadian Metallurgical Quarterly》1998,112(1):116
Adult rats that were isolated from the mother and nest for 1 hr per day from Postnatal Day 2 to 9 were studied. Controls consisted of handled littermates as well as separate litters that were never handled. As adults, animals were given either a pharmacological challenge (1.0 or 2.0 mg/kg amphetamine) or an environmental challenge (restraint). Previously isolated animals demonstrated increased activity compared to controls at both drug doses. Similarly, isolated animals manifested exaggerated inhibition of activity after restraint. Previously isolated animals usually did not show differences compared to controls under baseline conditions (saline injection or no restraint). The neuroplastic changes that result from the neonatal experience are long lasting and appear when the system is challenged. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
53.
Laura Menabò Alessandra Sansavini Antonella Brighi Grace Skrzypiec Annalisa Guarini 《Journal of Computer Assisted Learning》2021,37(6):1566-1577
Background: The rapid spread of COVID-19 forced many countries to adopt severe containment measures, transferring all didactic activities into virtual environments. However, the integration of technology in teaching may present difficulties, especially in some countries, such as Italy. Objectives: The present study analyzed how the two main factors of the Technology Acceptance Model (TAM), namely perceived usefulness and perceived ease of use, combined with online teaching self-efficacy, were associated with the intention to use technology to teach. We posited a moderated moderation model in which perceived usefulness represented the main predictor, with self-efficacy and perceived ease of use as moderators of intention to use technology to teach. Method: The model was tested through multiple regressions, using the PROCESS macro on SPSS 26 with a sample of 178 upper secondary school teachers in Italy. Results and conclusions: Regressions showed that each variable significantly predicted the intention to use technology. In addition, a moderation effect of self-efficacy on the perceived usefulness of using technology was found for medium and high-level of perceived ease of use of technology. Implications: The present study provides targeted implications for distance education policy and practice to promote its adoption (or the blended modality) in Italian upper schools. 相似文献
54.
Giuseppina Emanuela Grieco Noemi Brusco Giada Licata Daniela Fignani Caterina Formichi Laura Nigi Guido Sebastiani Francesco Dotta 《International journal of molecular sciences》2021,22(2)
Diabetes mellitus is a group of heterogeneous metabolic disorders characterized by chronic hyperglycaemia mainly due to pancreatic β cell death and/or dysfunction, caused by several types of stress such as glucotoxicity, lipotoxicity and inflammation. Different patho-physiological mechanisms driving β cell response to these stresses are tightly regulated by microRNAs (miRNAs), a class of negative regulators of gene expression, involved in pathogenic mechanisms occurring in diabetes and in its complications. In this review, we aim to shed light on the most important miRNAs regulating the maintenance and the robustness of β cell identity, as well as on those miRNAs involved in the pathogenesis of the two main forms of diabetes mellitus, i.e., type 1 and type 2 diabetes. Additionally, we acknowledge that the understanding of miRNAs-regulated molecular mechanisms is fundamental in order to develop specific and effective strategies based on miRNAs as therapeutic targets, employing innovative molecules. 相似文献
55.
Carmen de Jesús-Gil Lídia Sans-de San Nicols Ester Ruiz-Romeu Marta Ferran Laura Soria-Martínez Irene García-Jimnez Anca Chiriac Josep Manel Casanova-Seuma Josep Manel Fernndez-Armenteros Sherry Owens Antonio Celada Michael D. Howell Ramn María Pujol Luis Francisco Santamaria-Babí 《International journal of molecular sciences》2021,22(4)
Candida albicans (CA) infections have been associated with psoriasis onset or disease flares. However, the integrated immune response against this fungus is still poorly characterized in psoriasis. We studied specific immunoglobulins in plasma and the CA response in cocultures of circulating memory CD45RA− cutaneous lymphocyte antigen (CLA)+/− T cell with autologous epidermal cells from plaque and guttate psoriasis patients (cohort 1, n = 52), and also healthy individuals (n = 17). A complete proteomic profile was also evaluated in plaque psoriasis patients (cohort 2, n = 114) regarding their anti-CA IgA levels. Increased anti-CA IgA and IgG levels are present in the plasma from plaque but not guttate psoriasis compared to healthy controls. CA cellular response is confined to CLA+ T cells and is primarily Th17. The levels of anti-CA IgA are directly associated with CLA+ Th17 response in plaque psoriasis. Proteomic analysis revealed distinct profiles in psoriasis patients with high anti-CA IgA. C-C motif chemokine ligand 18, chitinase-3-like protein 1 and azurocidin were significantly elevated in the plasma from plaque psoriasis patients with high anti-CA levels and severe disease. Our results indicate a mechanism by which Candida albicans exposure can trigger a clinically relevant IL-17 response in psoriasis. Assessing anti-CA IgA levels may be useful in order to evaluate chronic psoriasis patients. 相似文献
56.
57.
Ignacio Hernandez Laura Tesoro Rafael Ramirez-Carracedo Javier Diez-Mata Sandra Sanchez Marta Saura Jose Luis Zamorano Carlos Zaragoza Laura Botana 《International journal of molecular sciences》2021,22(6)
In response to cardiac ischemia/reperfusion, proteolysis mediated by extracellular matrix metalloproteinase inducer (EMMPRIN) and its secreted ligand cyclophilin-A (CyPA) significantly contributes to cardiac injury and necrosis. Here, we aimed to investigate if, in addition to the effect on the funny current (I(f)), Ivabradine may also play a role against cardiac necrosis by reducing EMMPRIN/CyPA-mediated cardiac inflammation. In a porcine model of cardiac ischemia/reperfusion (IR), we found that administration of 0.3 mg/kg Ivabradine significantly improved cardiac function and reduced cardiac necrosis by day 7 after IR, detecting a significant increase in cardiac CyPA in the necrotic compared to the risk areas, which was inversely correlated with the levels of circulating CyPA detected in plasma samples from the same subjects. In testing whether Ivabradine may regulate the levels of CyPA, no changes in tissue CyPA were found in healthy pigs treated with 0.3 mg/kg Ivabradine, but interestingly, when analyzing the complex EMMPRIN/CyPA, rather high glycosylated EMMPRIN, which is required for EMMPRIN-mediated matrix metalloproteinase (MMP) activation and increased CyPA bonding to low-glycosylated forms of EMMPRIN were detected by day 7 after IR in pigs treated with Ivabradine. To study the mechanism by which Ivabradine may prevent secretion of CyPA, we first found that Ivabradine was time-dependent in inhibiting co-localization of CyPA with the granule exocytosis marker vesicle-associated membrane protein 1 (VAMP1). However, Ivabradine had no effect on mRNA expression nor in the proteasome and lysosome degradation of CyPA. In conclusion, our results point toward CyPA, its ligand EMMPRIN, and the complex CyPA/EMMPRIN as important targets of Ivabradine in cardiac protection against IR. 相似文献
58.
59.
Silvia Saturio Alicja M. Nogacka Marta Surez Nuria Fernndez Laura Mantecn Leonardo Mancabelli Christian Milani Marco Ventura Clara G. de los Reyes-Gaviln Gonzalo Solís Silvia Arboleya Miguel Gueimonde 《International journal of molecular sciences》2021,22(7)
The establishment of the gut microbiota poses implications for short and long-term health. Bifidobacterium is an important taxon in early life, being one of the most abundant genera in the infant intestinal microbiota and carrying out key functions for maintaining host-homeostasis. Recent metagenomic studies have shown that different factors, such as gestational age, delivery mode, or feeding habits, affect the gut microbiota establishment at high phylogenetic levels. However, their impact on the specific bifidobacterial populations is not yet well understood. Here we studied the impact of these factors on the different Bifidobacterium species and subspecies at both the quantitative and qualitative levels. Fecal samples were taken from 85 neonates at 2, 10, 30, 90 days of life, and the relative proportions of the different bifidobacterial populations were assessed by 16S rRNA–23S rRNA internal transcribed spacer (ITS) region sequencing. Absolute levels of the main species were determined by q-PCR. Our results showed that the bifidobacterial population establishment is affected by gestational age, delivery mode, and infant feeding, as it is evidenced by qualitative and quantitative changes. These data underline the need for understanding the impact of perinatal factors on the gut microbiota also at low taxonomic levels, especially in the case of relevant microbial populations such as Bifidobacterium. The data obtained provide indications for the selection of the species best suited for the development of bifidobacteria-based products for different groups of neonates and will help to develop rational strategies for favoring a healthy early microbiota development when this process is challenged. 相似文献
60.
Margaret Ottaviano Emilio Francesco Giunta Marianna Tortora Marcello Curvietto Laura Attademo Davide Bosso Cinzia Cardalesi Mario Rosanova Pietro De Placido Erica Pietroluongo Vittorio Riccio Brigitta Mucci Sara Parola Maria Grazia Vitale Giovannella Palmieri Bruno Daniele Ester Simeone 《International journal of molecular sciences》2021,22(7)
As widely acknowledged, 40–50% of all melanoma patients harbour an activating BRAF mutation (mostly BRAF V600E). The identification of the RAS–RAF–MEK–ERK (MAP kinase) signalling pathway and its targeting has represented a valuable milestone for the advanced and, more recently, for the completely resected stage III and IV melanoma therapy management. However, despite progress in BRAF-mutant melanoma treatment, the two different approaches approved so far for metastatic disease, immunotherapy and BRAF+MEK inhibitors, allow a 5-year survival of no more than 60%, and most patients relapse during treatment due to acquired mechanisms of resistance. Deep insight into BRAF gene biology is fundamental to describe the acquired resistance mechanisms (primary and secondary) and to understand the molecular pathways that are now being investigated in preclinical and clinical studies with the aim of improving outcomes in BRAF-mutant patients. 相似文献