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941.
F Wang RG Widejko Z Yang KT Nguyen H Chen LP Fernando KA Christensen JN Anker 《Analytical chemistry》2012,84(18):8013-8019
Sensors based upon surface-enhanced Raman spectroscopy (SERS) are attractive because they have narrow, vibrationally specific spectral peaks that can be excited using red and near-infrared light which avoids photobleaching, penetrates tissue, and reduces autofluorescence. Several groups have fabricated pH nanosensors by functionalizing silver or gold nanoparticle surfaces with an acidic molecule and measuring the ratio of protonated to deprotonated Raman bands. However, a limitation of these sensors is that macromolecules in biological systems can adsorb onto the nanoparticle surface and interfere with measurements. To overcome this interference, we encapsulated pH SERS sensors in a 30 nm thick silica layer with small pores which prevented bovine serum albumin (BSA) molecules from interacting with the pH-indicating 4-mercaptobenzoic acid (4-MBA) on the silver surfaces but preserved the pH-sensitivity. Encapsulation also improved colloidal stability and sensor reliability. The noise level corresponded to less than 0.1 pH units from pH 3 to 6. The silica-encapsulated functionalized silver nanoparticles (Ag-MBA@SiO(2)) were taken up by J774A.1 macrophage cells and measured a decrease in local pH during endocytosis. This strategy could be extended for detecting other small molecules in situ. 相似文献
942.
Michael E. McConney Kyle D. Anderson Lawrence L. Brott Rajesh R. Naik Vladimir V. Tsukruk 《Advanced functional materials》2009,19(16):2527-2544
Bioinspired design is an engineering approach that involves working to understand the design principles and strategies employed by biology in order to benefit the development of engineered systems. From a materials perspective, biology offers an almost limitless source of novel approaches capable of arousing innovation in every aspect of materials, including fabrication, design, and functionality. Here, recent and ongoing work on the study of bioinspired materials for sensing applications is presented. Work presented includes the study of fish flow receptor structures and the subsequent development of similar structures to improve flow sensor performance. The study of spider air‐flow receptors and the development of a spider‐inspired flexible hair is also discussed. Lastly, the development of flexible membrane based infrared sensors, highly influenced by the fire beetle, is presented, where a pneumatic mechanism and a thermal‐expansion stress‐mediated buckling‐based mechanism are investigated. Other areas that are discussed include novel biological signal filtering mechanisms and reciprocal benefits offered through applying the biology lessons to engineered systems. 相似文献
943.
Grazul-Bilska AT Navanukraw C Johnson ML Arnold DA Reynolds LP Redmer DA 《Reproduction (Cambridge, England)》2006,132(4):579-587
This study was conducted to evaluate the expression of endothelial nitric oxide synthase (eNOS) in ovarian follicles and corpora lutea (CL) throughout the estrous cycle in sheep. Three experiments were conducted to (1) immunolocalize eNOS protein, (2) determine expression of mRNA for eNOS and its receptor guanylate cyclase 1 soluble beta3 (GUCY1B3), and (3) co-localize eNOS and vascular endothelial growth factor (VEGF) proteins in the follicles and/or CL throughout the estrous cycle. In experiment 1, ovaries were collected from ewes treated with FSH, to induce follicular growth or atresia. In experiment 2, ovaries were collected from ewes treated with FSH and hCG to induce follicular growth and ovulation. In experiment 3, ovaries were collected from superovulated ewes to generate multiple CL on days 2, 4, 10, and 15 of the estrous cycle. In experiments 1 and 2, the expression of eNOS protein was detected in the blood vessels of the theca externa and interna of healthy ovarian follicles. However, in early and advanced atretic follicles, eNOS protein expression was absent or reduced. During the immediate postovulatory period, eNOS protein expression was detected in thecal-derived cells that appeared to be invading the granulosa layer. Expression of eNOS mRNA tended to increase in granulosa cells at 12 and 24 h, and in theca cells 48 h after hCG injection. In experiment 3, eNOS protein was located in the blood vessels of the CL during the estrous cycle. Dual localization of eNOS and VEGF proteins in the CL demonstrated that both were found in the blood vessels. 相似文献
944.
Dynes JJ Tyliszczak T Araki T Lawrence JR Swerhone GD Leppard GG Hitchcock AP 《Environmental science & technology》2006,40(5):1556-1565
A scanning transmission X-ray microscope illuminated with synchrotron light was used to investigate the speciation and spatial distributions of metals in a microbial biofilm cultivated from river water. Metal 2p absorption edge signals were used to provide metal speciation (through shapes of the absorption spectra) and quantitative spatial distributions of the metal species. This paper presents sample data and describes methods for extracting quantitative maps of metal species from image sequences recorded in the region of the metal 2p edges. Comparisons were made with biochemical characterization of the same region using images recorded at the C 1s and O 1s edges. The method is applied to detailed quantitative analysis of ferrous and ferric iron in a river biofilm, in concert with mapping Ni(II) and Mn(II) species in the same region. The distributions of the metal species are discussed in the context of the biofilm structure. These results demonstrate that soft X-ray STXM measurements at the metal 2p absorption edges can be used to speciate metals and to provide quantitative spatial distribution maps for metal species in environmental samples with 50 nm spatial resolution. 相似文献
945.
The aim of this study was to assess the effect of grazing (G) vs. zero-grazing (ZG), level of milk production, and quality and type of housing system [free stalls (FS) and straw yards (SY)] on the prevalence of lameness and leg injuries in dairy cows. Observations were made on 37 commercial dairy farms across Great Britain. A single visit of 5 d duration was made to each farm. During this visit, lameness scores and the incidence of swellings, rubs, and injuries to hocks and knees were recorded on all the peak- or mid-lactation cows. Aspects of the quality of housing and management that were likely to affect foot and leg health were recorded. There were more lame cows on ZG farms (39 ± 0.02%) than on grazing (G) farms (15 ± 0.01%), and lameness scores were higher on FS farms compared with SY farms (0.25 ± 0.01 vs. 0.05 ± 0.01). Cows on SY farms had fewer hock and knee injuries compared with FS farms. The frequency of knee swellings was higher on ZG farms (0.31 ± 0.02) than on G farms (0.15 ± 0.01). Aspects of the free-stall design affected foot and leg health. The number of hock swellings increased with increasing stall gradient (0.16 ± 0.01 with no slope vs. 0.39 ± 0.02 at a 0 to 1.5% slope). There was an interaction between the length of the free-stall lunging space and the hip width of the cow, indicating that the incidence of lameness is generally highest on farms with small free stalls and heavy cows. High levels of milk production did not affect lameness or leg injury. The results indicate that housing cows throughout the year potentially has a detrimental effect on foot and leg health. However, good free-stall design may reduce lameness and leg lesions. 相似文献
946.
The flame synthesis of carbon nanotubes (CNTs) coupled with application of moderate electric fields is studied experimentally as a means to control CNTs growth rates and morphology. The nanotubes are grown on a conductive metal-based catalytic probe positioned at the fuel side of the opposed flow oxy-flame. The probe was connected to an external voltage source to generate radial electric fields on its surface. At low applied voltages (from 0.3 to 2 V), the effect of the electric field on alignment and growth rate enhancement revealed the generation of vertically aligned carbon nanotube (VACNT) arrays with uniform distribution of CNT diameters. Further increases of the applied voltage resulted in structural modifications of the generated nanotubes. In particular, helically coiled CNTs were observed at applied voltages of 3 V. At higher voltages the arrays contained multi-walled CNTs with fascinating modified morphologies such as Y, T, and multi-junction patterns. Analysis of the samples generated at applied voltage of 5 V showed the presence of particle sprouting and early CNT junctions in the form of small bumps extruding from the outer surface of the CNTs. Analysis of material samples synthesized at 12 and 25 V showed the presence of fully branched CNT structures. 相似文献
947.
948.
Xi Long W Louis Cleveland Y Lawrence Yao 《IEEE transactions on information technology in biomedicine》2005,9(3):407-412
In this paper, we describe a novel strategy for combining fisher's linear discriminant (FLD) preprocessing with a feedforward neural network to classify cultured cells in bright field images. This technique was applied to various experimental scenarios utilizing different imaging environments, and the results were compared with those for the traditional principal component analysis (PCA) preprocessing. Our FLD preprocessing was shown to be more effective than PCA due in large part to the fact that FLD maximizes the ratio of between-class to within-class scatter. The new cell recognition algorithm with FLD preprocessing improves accuracy while the speed is suitable for practical applications. 相似文献
949.
Lawrence E. Eiselstein Duane Steffey Andrew Nissan Nigel Corlett Roberto Dugnani Esra Kus Sarah G. Stewart 《Journal of Materials Engineering and Performance》2009,18(5-6):768-780
ASTM F 2129 test method nor the FDA provides any guidance as to what constitutes an acceptance criterion for the corrosion resistance of implantable medical devices. Neither provide any guidance on how many samples to test or how to handle censored data, i.e. datasets where there are only a few tests that breakdown. The development of both a statistically valid acceptance criterion for corrosion resistance and a method of evaluation would be of significant benefit to the medical device community. This study of 420 nitinol cyclic polarization tests, which builds on previous research that was presented at SMST 2007, investigates the effect of long-term exposure to simulated in vivo environments with differing degrees of aeration. This was accomplished by pre-exposing electropolished (EP) nitinol to phosphate buffered saline (PBS) at 37 °C that had been sparged with either ultra high purity nitrogen or laboratory air. Immersion times ranged from 1 h up to 30 days. A total of 290 EP samples were tested in order to obtain a reasonable number of samples with breakdown, i.e. pitted. In addition, a total of 130 mechanical polished (MP) samples were also analyzed. This data allow us to test our statistical model that was presented at SMST 2007. This model takes into account the probability of breakdown per unit of exposed surface area and, if breakdown occurs, predicts the probability that E b ? E r is greater than some threshold value. Aerated PBS environments were found to have a large influence on the margin of safety against pitting in vivo. Statistical methods for treating highly right censored pitting data are presented. 相似文献
950.