Peptidyl Virus-Like Nanovesicles as Reconfigurable “Trojan Horse” for Targeted siRNA Delivery and Synergistic Inhibition of Cancer Cells

The self-assembly of peptidyl virus-like nanovesicles (pVLNs) composed of highly ordered peptide bilayer membranes that encapsulate the small interfering RNA (siRNA) is reported. The targeting and enzyme-responsive sequences on the bilayer's surface allow the pVLNs to enter cancer cells with high efficiency and control the release of genetic drugs in response to the subcellular environment. By transforming its structure in response to the highly expressed enzyme matrix metalloproteinase 7 (MMP-7) in cancer cells, it helps the siRNA escape from the lysosomes, resulting in a final silencing efficiency of 92%. Moreover, the pVLNs can serve as reconfigurable “Trojan horse” by transforming into membranes triggered by the MMP-7 and disrupting the cytoplasmic structure, thereby achieving synergistic anticancer effects and 96% cancer cell mortality with little damage to normal cells. The pVLNs benefit from their biocompatibility, targeting, and enzyme responsiveness, making them a promising platform for gene therapy and anticancer therapy.     

Morphological Models for the Collapse of Area Features in Digital Map Generalization

Collapse is an essential operation for the manipulation of area features in digital data generalization. This operation can be categorized into two types: complete collapse and partial collapse. The former is composed of another two types: area-to-point and area-to-line collapse. In this paper, a set of algebraic models built upon the operators in mathematical morphology is described for the area-to-line collapse and partial collapse operations. For the area-to-line collapse operation, a modified skeleton algorithm is presented. For the partial collapse operation, a procedure is designed which consists of a set of operations, i.e., the skeletonization, separation of areal and linear parts, simplification of areas and an overlay operation. These models are tested using real map data sets.     

舒曼钢琴音乐的双重性格特征

德国作曲家罗伯特·舒曼是十九世纪上半叶欧洲音乐文化史上最突出的人物之一。在他的钢琴音乐创作中,深刻地反映出浪漫主义音乐的特点。舒曼设计了一个想象中的“大卫同盟”,其中有两名核心盟员,一个叫弗洛列斯坦,另一个叫埃塞比乌斯。前者性格热情奔放,锋芒毕露;后者则文雅娴静,富于幻想,实际上这就是舒曼本人性格中的两个侧面。这种双重性格特征在舒曼的钢琴音乐中得到充分展示,并成为舒曼钢琴音乐的艺术特征之一。     

改革实践教学模式、培养学生综合能力──增设给水排水专业质量检测实习的体会

对给排水专业在优化专业教学体系,深化专科教学模式及教学内容改革中,实施的四次大型综合性实践教学,进行了认真总结．经过改变随课跟进的实验教学旧模式,在教学计划中增设了“水质指标综合测试与水质评价”实习内容使分散的教学实验得以“水质监测与评价”为骨架,有机穿插在实践教学中,为专科学校培养复合应用型人才,进行了有益的探索与实践,并获得较好效果．     

The first measurement of an absolute surface concentration of reaction intermediates in ethylene hydrogenation

The first measurement of a turnover rate with respect to surface intermediate concentration in a high pressure heterogeneous catalytic reaction is reported. By using infrared-visible sum frequency generation to study the hydrogenation of ethylene on Pt(111), it was found that the surface concentration of -bonded ethylene, the key reaction intermediate, represented approximately 4% of a monolayer. Thus the absolute turnover rate per surface adsorbed ethylene molecule is 25 times faster than the rate measured per platinum atom. To explain these results, we propose a model of weakly adsorbed ethylene intermediates reacting on atop sites.     

Dynamically modulated intensity interrogation scheme using waveguide coupled surface plasmon resonance sensors

An electro-optically modulated intensity interrogation method based on tunable waveguide coupled surface plasmon resonance sensors has been proposed. It has been theoretically and experimentally demonstrated that the proposed scheme can enable sensitive measurement of measurand variations. By modulating the refractive index in the waveguide layer, this interrogation method yields modulated signal whose amplitude is related to measurand's refractive index. This amplitude modulated signal offers a higher signal to noise ratio and eliminates additive noise in the sensor system. A preliminary investigation using saline buffers with different NaCl concentrations shows a resolution of 2.3 × 10^?6 refractive index unit by our approach. Resolution can be controlled by the amplitude of the applied modulation voltage and can be further enhanced by optimizing the device structure or improving the electro-optical (E-O) coefficient of the E-O material. This approach is simple, stable, and promising for low-cost or multi-channel SPR biosensor applications.     

浅析艺术类专业《计算机基础》的教学方法

近年来,高校的计算机基础教学在不断发展,教学方法也在不断地改进。如何根据学生特点,使用各种行之有效的教学方法是提高教学质量的关键。文章结合教学实际,分析探讨了如何针对艺术类专业学生的特点,进行《计算机基础》教学方法改革。     

Semi-supervised locally discriminant projection for classification and recognition

Semi-supervised dimensional reduction methods play an important role in pattern recognition, which are likely to be more suitable for plant leaf and palmprint classification, since labeling plant leaf and palmprint often requires expensive human labor, whereas unlabeled plant leaf and palmprint is far easier to obtain at very low cost. In this paper, we attempt to utilize the unlabeled data to aid plant leaf and palmprint classification task with the limited number of the labeled plant leaf or palmprint data, and propose a semi-supervised locally discriminant projection (SSLDP) algorithm for plant leaf and palmprint classification. By making use of both labeled and unlabeled data in learning a transformation for dimensionality reduction, the proposed method can overcome the small-sample-size (SSS) problem under the situation where labeled data are scant. In SSLDP, the labeled data points, combined with the unlabeled data ones, are used to construct the within-class and between-class weight matrices incorporating the neighborhood information of the data set. The experiments on plant leaf and palmprint databases demonstrate that SSLDP is effective and feasible for plant leaf and palmprint classification.     

语义驱动的关联规则挖掘算法研究

为了使传统的关联规则挖掘算法在结合到具体领域时具有更强的适应性,提出了DS-Apriori算法。该算法建立在语义本体的基础上,根据项集内部的语义相关度动态的确定该项集的最小支持度,并采用了项集语义相关度的增量计算方法。实验结果表明,DS-Apriori算法在很大程度上提高了关联规则挖掘算法的效率和效果。     

动态相位调整技术在FPGA中的设计与实现

提出一种基于FPGA的动态相位调整实现方案。在高速数据传输接口中,由于数据窗缩小以及传输路径不一致,造成数据和时钟信号在FPGA的接收端发生位偏移和字偏移。动态相位调整技术根据当前各数据线物理状态,对各信号线动态进行去偏移操作,克服了静态相位调整中参数不可再调的缺点,使接口不断适应外部环境的变化,从而保证数据的可靠传输。