全文获取类型
收费全文 | 681篇 |
免费 | 55篇 |
国内免费 | 5篇 |
专业分类
电工技术 | 3篇 |
综合类 | 1篇 |
化学工业 | 231篇 |
金属工艺 | 8篇 |
机械仪表 | 19篇 |
建筑科学 | 24篇 |
能源动力 | 19篇 |
轻工业 | 132篇 |
水利工程 | 13篇 |
石油天然气 | 13篇 |
无线电 | 46篇 |
一般工业技术 | 84篇 |
冶金工业 | 27篇 |
原子能技术 | 5篇 |
自动化技术 | 116篇 |
出版年
2024年 | 3篇 |
2023年 | 20篇 |
2022年 | 22篇 |
2021年 | 42篇 |
2020年 | 40篇 |
2019年 | 51篇 |
2018年 | 35篇 |
2017年 | 46篇 |
2016年 | 39篇 |
2015年 | 37篇 |
2014年 | 35篇 |
2013年 | 67篇 |
2012年 | 39篇 |
2011年 | 48篇 |
2010年 | 33篇 |
2009年 | 31篇 |
2008年 | 28篇 |
2007年 | 15篇 |
2006年 | 16篇 |
2005年 | 9篇 |
2004年 | 11篇 |
2003年 | 10篇 |
2002年 | 6篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 1篇 |
1998年 | 5篇 |
1997年 | 3篇 |
1996年 | 7篇 |
1995年 | 4篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 5篇 |
1990年 | 5篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1976年 | 1篇 |
1972年 | 1篇 |
1961年 | 1篇 |
排序方式: 共有741条查询结果,搜索用时 0 毫秒
61.
Abdolkarim Abbaspour Leila Baramakeh S. Masoud Nabavizadeh 《Electrochimica acta》2007,52(14):4798-4803
The electrocatalytic oxidation of guanine and DNA is demonstrated on a sol-gel coated carbon screen printed electrode modified with {MeReO(edt)}2 using cyclic and differential pulse voltammetric techniques. An oxidation peak at 370 mV was found, but no corresponding reduction peaks could be detected in the negative scan, which indicates that the oxidation of guanine is completely irreversible process. The oxidation peak potentials are shifted to more negative values with increasing pH. The utility of applying the sensor for determination of guanine and ss-DNA were investigated. The linear ranges were 0.19-10.8 and 0.45-7.8 μg ml−1 for guanine and DNA, respectively. Detection limits of 0.1 and 0.32 μg ml−1 were obtained for guanine and ss-DNA, respectively. 相似文献
62.
This paper discusses a fundamental problem in natural language generation: how to organize the content of a text in a coherent and natural way. In this research, we set out to determine the semantic content and the rhetorical structure of texts and to develop heuristics to perform this process automatically within a text generation framework. The study was performed on a specific language and textual genre: French instructional texts. From a corpus analysis of these texts, we determined nine senses typically communicated in instructional texts and seven rhetorical relations used to present these senses. From this analysis, we then developed a set of presentation heuristics that determine how the senses to be communicated should be organized rhetorically in order to create a coherent and natural text. The heuristics are based on five types of constraints: conceptual, semantic, rhetorical, pragmatic, and intentional constraints. To verify the heuristics, we developed the spin natural language generation system, which performs all steps of text generation but focuses on the determination of the content and the rhetorical structure of the text. 相似文献
63.
To meet the ever-increasing construction demands around the world during recent years,reinforcement and stabilization methods have been widely used by geotechnical engineers to improve the performances and behavior of fine-grained soils.Although lime stabilization increases the compressive strength of soils,it reduces the soil ductility at the same time.Recent research shows that random fiber inclusion modifies the brittleness of soils.In the current research,the effects of lime and polypropylene(PP) fiber additions on such characteristics as compressive and shear strengths,failure strain,secant modulus of elasticity(E_(50)) and shear strength parameters of mixtures were investigated.Kaolinite was treated with 1%,3% and 5% lime by dry weight of soil and reinforced with 0.1% monovalent PP fibers with the length of 6 mm.Samples were prepared at optimum conditions and cured at 35℃ for 1 d,7 d and28 d at 90% relative humidity and subsequently subjected to uniaxial and triaxial compression tests(UCT and TCT) under cell pressures of 25 kPa,50 kPa and 100 kPa.Results showed that inclusion of random PP fibers to clay-lime mixtures increases both compressive and shear strengths as well as the ductility.Lime content and curing period were found to be the most influential factors.Scanning electron microscopy(SEM) analysis showed that lime addition and the formation of cementitious compounds bind soil particles and increase soil/fiber interactions at interface,leading to enhanced shear strength.The more ductile the stabilized and reinforced composition,the less the cracks in roads and waste landfill covers. 相似文献
64.
Application of a new xylanase activity from Bacillus amyloliquefaciens XR44A in brewer's spent grain saccharification 下载免费PDF全文
65.
Inhibitors of Polyhydroxyalkanoate (PHA) Synthases: Synthesis,Molecular Docking,and Implications 下载免费PDF全文
Dr. Wei Zhang Dr. Chao Chen Ruikai Cao Dr. Leila Maurmann Prof. Ping Li 《Chembiochem : a European journal of chemical biology》2015,16(1):156-166
Polyhydroxyalkanoate (PHA) synthases (PhaCs) catalyze the formation of biodegradable PHAs that are considered to be ideal alternatives to non‐biodegradable synthetic plastics. However, study of PhaCs has been challenging because the rate of PHA chain elongation is much faster than that of initiation. This difficulty, along with lack of a crystal structure, has become the main hurdle to understanding and engineering PhaCs for economical PHA production. Here we report the synthesis of two carbadethia CoA analogues—sT‐CH2‐CoA ( 26 a ) and sTet‐CH2‐CoA ( 26 b )—as well as sT‐aldehyde (saturated trimer aldehyde, 29 ), as new PhaC inhibitors. Study of these analogues with PhaECAv revealed that 26 a / b and 29 are competitive and mixed inhibitors, respectively. Both the CoA moiety and extension of PHA chain will increase binding affinity; this is consistent with our docking study. Estimation of the Kic values of 26 a and 26 b predicts that a CoA analogue incorporating an octameric hydroxybutanoate (HB) chain might facilitate the formation of a kinetically well‐behaved synthase. 相似文献
66.
Jaehak Jeong Narayanan Kannan Jeff Arnold Roger Glick Leila Gosselink Raghavan Srinivasan 《Water Resources Management》2010,24(15):4505-4527
Increasing urbanization changes runoff patterns to be flashy and instantaneous with decreased base flow. A model with the
ability to simulate sub-daily rainfall–runoff processes and continuous simulation capability is required to realistically
capture the long-term flow and water quality trends in watersheds that are experiencing urbanization. Soil and Water Assessment
Tool (SWAT) has been widely used in hydrologic and nonpoint sources modeling. However, its subdaily modeling capability is
limited to hourly flow simulation. This paper presents the development and testing of a sub-hourly rainfall–runoff model in
SWAT. SWAT algorithms for infiltration, surface runoff, flow routing, impoundments, and lagging of surface runoff have been
modified to allow flow simulations with a sub-hourly time interval as small as one minute. Evapotranspiration, soil water
contents, base flow, and lateral flow are estimated on a daily basis and distributed equally for each time step. The sub-hourly
routines were tested on a 1.9 km2 watershed (70% undeveloped) near Lost Creek in Austin Texas USA. Sensitivity analysis shows that channel flow parameters
are more sensitive in sub-hourly simulations (Δt = 15 min) while base flow parameters are more important in daily simulations (Δt = 1 day). A case study shows that the sub-hourly SWAT model reasonably reproduces stream flow hydrograph under multiple storm
events. Calibrated stream flow for 1 year period with 15 min simulation (R
2 = 0.93) shows better performance compared to daily simulation for the same period (R
2 = 0.72). A statistical analysis shows that the improvement in the model performance with sub-hourly time interval is mostly
due to the improvement in predicting high flows. The sub-hourly version of SWAT is a promising tool for hydrology and non-point
source pollution assessment studies, although more development on water quality modeling is still needed. 相似文献
67.
Omid Velayatipour Leila Nikzad Mohammad Farvizi 《International Journal of Applied Ceramic Technology》2023,20(3):1667-1680
In this study, NiTi–x wt.% B4C (x = 0, 2, and 4) composites were consolidated with spark plasma sintering method, and the effects of boron carbide reinforcement addition on the microstructure and wear behavior of samples were investigated. Identification of the constituent phases of samples by the X-ray diffraction method plus Rietveld analysis revealed that the stability of the martensite phase increased in the composite samples because of mismatch stresses between the NiTi matrix phase and the reinforcing particles, which increases the density of the dislocations and facilitates the diffusion process that subsequently leads to the formation of stable intermetallics. The results of hardness test indicated that the hardness value increased from 3.67 GPa for pure NiTi to 10.99 GPa for NiTi–4 wt.% B4C. Results of wear test revealed that boron carbide reinforced composite specimens had higher wear resistance, whereas wear rate of NiTi sample was 3.6 × 10−3 mm3/N m, and it reached to .21 × 10−3 mm3/N m for NiTi–4 wt.% B4C. Investigation of microstructure by scanning electron microscopy images and EDS analysis revealed that the wear mechanism in NiTi samples was abrasive and the addition of B4C to NiTi changed the wear mechanisms from abrasive to a combination of oxidation, adhesive, and delamination mechanisms. 相似文献
68.
Shabnam Asghari Niari Reza Rahbarghazi Roya Salehi Leila Kazemi Sonia Fathi Karkan Mohammad Karimipour 《Microscopy research and technique》2022,85(4):1433-1443
In recent years with regard to the development of nanotechnology and neural stem cell discovery, the combinatorial therapeutic strategies of neural progenitor cells and appropriate biomaterials have raised the hope for brain regeneration following neurological disorders. This study aimed to explore the proliferation and neurogenic effect of PLGA and PLGA–PEG nanofibers on human SH-SY5Y cells in in vitro condition. Nanofibers of PLGA and PLGA–PEG biomaterials were synthesized and fabricated using electrospinning method. Physicochemical features were examined using HNMR, FT-IR, and water contact angle assays. Ultrastructural morphology, the orientation of nanofibers, cell distribution and attachment were visualized by SEM imaging. Cell survival and proliferation rate were measured. Differentiation capacity was monitored by immunofluorescence staining of Map-2. HNMR, FT-IR assays confirmed the integration of PEG to PLGA backbone. Water contact angel assay showed increasing surface hydrophilicity in PLGA–PEG biomaterial compared to the PLGA substrate. SEM analysis revealed the reduction of PLGA–PEG nanofibers' diameter compared to the PLGA group. Cell attachment was observed in both groups while PLGA–PEG had a superior effect in the promotion of survival rate compared to other groups (p < .05). Compared to the PLGA group, PLGA–PEG increased the number of Ki67+ cells (p < .01). PLGA–PEG biomaterial induced neural maturation by increasing protein Map-2 compared to the PLGA scaffold in a three-dimensional culture system. According to our data, structural modification of PLGA with PEG could enhance orientated differentiation and the dynamic growth of neural cells. 相似文献
69.
Rieth Marta A Moreira Marina B Fuchs Flávio D Moreira Leila B Fuchs Sandra C 《Nutrition journal》2012,11(1):1-7
Background
Pseudomonas aeruginosa adhesion to animal/human cells for infection establishment involves adhesive proteins, including its galactose- and fucose-binding lectins PA-IL (LecA) and PA-IIL (LecB). The lectin binding to the target-cell receptors may be blocked by compatible glycans that compete with those of the receptors, functioning as anti-adhesion glycodecoys. The anti-adhesion treatment is of the utmost importance for abrogating devastating antibiotic-resistant P. aeruginosa infections in immunodeficient and cystic fibrosis (CF) patients. This strategy functions in nature in protecting embryos and neonates. We have shown that PA-IL, PA-IIL, and also CV-IIL (a PA-IIL homolog produced in the related pathogen Chromobacterium violaceum) are highly useful for revealing natural glycodecoys that surround embryos in diverse avian eggs and are supplied to neonates in milks and royal jelly. In the present study, these lectins were used as probes to search for seed embryo-protecting glycodecoys.Methods
The lectin-blocking glycodecoy activities were shown by the hemagglutination-inhibition test. Lectin-binding glycoproteins were detected by Western blotting with peroxidase-labeled lectins.Results
The present work reports the finding - by using PA-IL, PA-IIL, and CV-IIL - of rich glycodecoy activities of low (< 10 KDa) and high MW (> 10 kDa) compounds (including glycoproteins) in extracts of cashew, cocoa, coffee, pumpkin, and tomato seeds, resembling those of avian egg whites, mammal milks, and royal jelly.Conclusions
Edible seed extracts possess lectin-blocking glycodecoys that might protect their embryos from infections and also might be useful for hampering human and animal infections. 相似文献70.
Martins LD Andrade MF Freitas ED Pretto A Gatti LV Albuquerque EL Tomaz E Guardani ML Martins MH Junior OM 《Environmental science & technology》2006,40(21):6722-6729
The objective of this study was to improve the vehicular emissions inventory for the light- and heavy-duty fleet in the metropolitan area of S?o Paulo (MASP), Brazil. To that end, we measured vehicle emissions in road tunnels located in the MASP. On March 22-26, 2004 and May 04-07, 2004, respectively, CO, CO2, NOx, SO2, and volatile organic compounds (VOCs) emissions were measured in two tunnels: the Janio Quadros, which carries light-dutyvehicles; and the Maria Maluf, which carries light-duty vehicles and heavy-duty diesel trucks. Pollutant concentrations were measured inside the tunnels, and background pollutant concentrations were measured outside of the tunnels. The mean CO and NOx emission factors (in g km(-1)) were, respectively, 14.6 +/- 2.3 and 1.6 +/- 0.3 for light-duty vehicles, compared with 20.6 +/- 4.7 and 22.3 +/- 9.8 for heavy-duty vehicles. The total VOCs emission factor for the Maria Maluf tunnel was 1.4 +/- 1.3 g km(-1). The main VOCs classes identified were aromatic, alkane, and aldehyde compounds. For the heavy-duty fleet, NOx emission factors were approximately 14 times higher than those found for the light-duty fleet. This was attributed to the high levels of NOx emissions from diesel vehicles. 相似文献