A kinetic analysis of the changes in fluorescence on the interaction of 8-anilinonaphthalene-1-sulphonate with submitochondrial particles

Residual deficiency of brain performance in chronic alcoholics after a 5-year abstinency. Brain performance of a group of 43 chronic alcoholics abstinent since 5 years was tested. Some aspects of higher intellectual performance were found to be reduced as compared to a control population. The performance was also better than that of a group of chronic alcoholics abstinent for only one year. The residual deficiency of performance could be interpreted as a "functional psychosyndrome", resulting from chronic sensory deprivation on the base of the alcoholism.     

Differences in attitudes toward sex-typed behavior of nonretarded and retarded children

The present study was an initial attempt at understanding gender constancy in retarded children. Stories about retarded or nonretarded boys or girls were given to teachers and caretakers of retarded children and teachers of nonretarded children. The stories described children in four sex-stereotyped activities. Respondents rated each child in the stories on scales of typicality and acceptability. Results indicated that, while nonretarded children were rated as typical when engaging in sex-appropriate, desirable play activities, retarded children were rated as typical when engaging in undesirable activities, regardless of sex appropriateness. Different professional groups appeared to use different criteria when judging the children in the stories.     

Pathogenicity of K. ozaenae for mice and chick embryos

The authors tested the virulence of K. ozaenae--its old museum strains, the freshly isolated ones and those passaged on meat-peptone agar; experiments were carried out on mice and chick embryos. To mice the culture was administered intraperitoneally, subcutaneously, intranasally, and to embryos--into the allantoic cavity and on the chorioallantoic membrane. Irrespective of the method of administration, the freshly isolated strains were highly virulent both for mice and for chick embryos. The virulence of such strains decreased in the process of passaging on the nutrient medium. Old museum strains were of low virulence for albino mice and avirulent for chick embryos. In comparing the virulent and avirulent strains there were found no differences in the antigenic structure and toxicity of the Boiven complex, cytoplasm, membrane, capsular polysaccharide or whole virulent and avirulent bacteria killed by heating.     

Distinct stability of recombinant L and H subunits of human ferritin: calorimetric and ANS binding studies

Thermodynamic and pH stability of recombinant human L- and H-ferritins wereprobed by differential scanning calorimetry and 8-anilino-1-naphthalenesulfonate (ANS) binding in the pH range 2-7. At pH 2.0-2.8 theywere dissociated into subunit monomers and in this pH interval theH-subunit displayed a single calorimetrically-revealed domain withproperties of a molten globule-like state: low enthalpy (6.3-8.0 J/g or169-172 kJ/mol) and Tm of thermal unfolding (approximately 50 degrees C), awide transition range (approximately 20 degrees C) and high ANS binding. Incontrast, at pH 2 the L-ferritin subunit showed two calorimetric domainswith Tm of 35 and 40 degrees C with similar unfolding enthalpies and withmoderate extent of interactions, as indicated by the ratio of calorimetricenthalpy (293.9 kJ/mol) and van't Hoff enthalpy (174.2 kJ/mol) for thethermal transition. A pH increase from 2.0 to 2.8 determined the couplingof the two domains into a single cooperative folding unit and drasticincrease of the transition temperature (from 37 to 80 degrees C). Thecontacts between the two domains in the L-subunit appeared to contribute toabout 30% of the total stabilization free energy. The unfolding enthalpies,heat capacity changes and pronounced ANS binding of the L-subunit at pH2.0- 2.8 indicated that part of the structure lacked 'meltable' tertiaryinteractions. The results indicate that H- and L-subunits are stabilized bylargely different intra-chain interactions with a critical contribution toL-subunit stability of embedded salt bridge(s) absent in the H-subunit.