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11.
Silvia Saturio Alicja M. Nogacka Marta Surez Nuria Fernndez Laura Mantecn Leonardo Mancabelli Christian Milani Marco Ventura Clara G. de los Reyes-Gaviln Gonzalo Solís Silvia Arboleya Miguel Gueimonde 《International journal of molecular sciences》2021,22(7)
The establishment of the gut microbiota poses implications for short and long-term health. Bifidobacterium is an important taxon in early life, being one of the most abundant genera in the infant intestinal microbiota and carrying out key functions for maintaining host-homeostasis. Recent metagenomic studies have shown that different factors, such as gestational age, delivery mode, or feeding habits, affect the gut microbiota establishment at high phylogenetic levels. However, their impact on the specific bifidobacterial populations is not yet well understood. Here we studied the impact of these factors on the different Bifidobacterium species and subspecies at both the quantitative and qualitative levels. Fecal samples were taken from 85 neonates at 2, 10, 30, 90 days of life, and the relative proportions of the different bifidobacterial populations were assessed by 16S rRNA–23S rRNA internal transcribed spacer (ITS) region sequencing. Absolute levels of the main species were determined by q-PCR. Our results showed that the bifidobacterial population establishment is affected by gestational age, delivery mode, and infant feeding, as it is evidenced by qualitative and quantitative changes. These data underline the need for understanding the impact of perinatal factors on the gut microbiota also at low taxonomic levels, especially in the case of relevant microbial populations such as Bifidobacterium. The data obtained provide indications for the selection of the species best suited for the development of bifidobacteria-based products for different groups of neonates and will help to develop rational strategies for favoring a healthy early microbiota development when this process is challenged. 相似文献
12.
Alessia Vignoli Emanuela Risi Amelia McCartney Ilenia Migliaccio Erica Moretti Luca Malorni Claudio Luchinat Laura Biganzoli Leonardo Tenori 《International journal of molecular sciences》2021,22(9)
Precision oncology is an emerging approach in cancer care. It aims at selecting the optimal therapy for the right patient by considering each patient’s unique disease and individual health status. In the last years, it has become evident that breast cancer is an extremely heterogeneous disease, and therefore, patients need to be appropriately stratified to maximize survival and quality of life. Gene-expression tools have already positively assisted clinical decision making by estimating the risk of recurrence and the potential benefit from adjuvant chemotherapy. However, these approaches need refinement to further reduce the proportion of patients potentially exposed to unnecessary chemotherapy. Nuclear magnetic resonance (NMR) metabolomics has demonstrated to be an optimal approach for cancer research and has provided significant results in BC, in particular for prognostic and stratification purposes. In this review, we give an update on the status of NMR-based metabolomic studies for the biochemical characterization and stratification of breast cancer patients using different biospecimens (breast tissue, blood serum/plasma, and urine). 相似文献
13.
Occurrence patterns of alien freshwater turtles in a large urban pond ‘Archipelago’ (Rome,Italy): Suggesting hypotheses on root causes 下载免费PDF全文
Maria Paola Di Santo Leonardo Vignoli Giuseppe M. Carpaneto Corrado Battisti 《Lakes & Reservoirs: Research and Management》2017,22(1):56-64
The patterns of the occurrence and distribution of alien freshwater turtle species in an urban pond archipelago (Rome, Italy) were analysed, with the aim of exploring the role of a set of factors (type of ponds, landscape context, size area, distance from the nearest road) with a generalized linear model approach. A total of 311 ponds subdivided in three types (fountains, small basins, lakes) embedded in different landscape contexts (public parks, private parks, urban areas) at differing distances from the nearest road were sampled. Six non‐native freshwater turtle species in 31 sites were recorded (9.97%). Lakes exhibited the highest occurrence rates of alien freshwater turtles, compared to small basins and fountains. Freshwater turtle species in urbanized areas were only observed in parks (both public and private). In both the public and private parks, the lakes exhibited the highest percentage of occupied sites, with fountains being the lowest. A direct and significant relationship was observed between pond size and species richness. The distance from the nearest road did not appear to affect species richness. A first interpretation of the data from this study facilitated the postulation of two a posteriori hypotheses that should be tested, as follows: (i) the causal process of turtle release is random, and the rate of extinction (and recapture) is higher in smaller ponds, thus producing the observed pattern; and (ii) the turtle release is not random, and people actively select the ponds they consider more suitable for their pet animals. In this study, it appears the lakes were perceived by those who abandon their pets as the most ecologically suitable habitats among other pond types to accommodate the different species of turtles. Knowledge of people's attitudes in regard to releasing pet animals also might assist managers of public green spaces to develop strategies aimed to preserve local biodiversity, and to educate the public about the conservation issue represented by the alien species. 相似文献
14.
Enrique J. Fernandez-Sanchez Leonardo Rubio Javier Diaz Eduardo Ros 《Machine Vision and Applications》2014,25(5):1211-1225
Background subtraction consists of segmenting objects in movement in a video captured by a static camera. This is typically performed using color information, but it leads to wrong estimations due to perspective and illumination issues. We show that multimodal approaches based on the integrated use of color and depth cues produce more accurate and robust results than using either data source independently. Depth is less affected by issues such as shadows or foreground objects similar to background. However, objects close to the background may not be detected when using only range information, being color information complementary in those cases. We propose an extension of a well-known background subtraction technique which fuses range and color information, as well as a post-processing mask fusion stage to get the best of each feature. We have evaluated the method proposed using a well-defined dataset and different disparity estimation algorithms, showing the benefits of our method for fusion color and depth cues. 相似文献
15.
In traditional networks special efforts are put to secure the perimeter with firewalls: particular routers that analyze and filter the traffic to separate zones with different levels of trust. In wireless multi-hop networks the perimeter is a concept extremely hard to identify, thus, it is much more effective to enforce control on the nodes that will route more traffic. But traffic filtering and traffic analysis are costly activities for the limited resources of mesh nodes, so a trade-off must be reached limiting the number of nodes that enforce them. This work shows how, using the OLSR protocol, the centrality of groups of nodes with reference to traffic can be estimated with high accuracy independently of the network topology or size. We also show how this approach greatly limits the impact of an attack to the network using a number of firewalls that is only a fraction of the available nodes. 相似文献
16.
17.
Heloise O. Pastore Alberto Frache Enrico Boccaleri Leonardo Marchese Giovanni Camino 《大分子材料与工程》2004,289(9):783-786
Summary: The success of the use of layered silicates in polymer nanocomposites, to improve physical and chemical properties is strictly related to a deeper knowledge of the mechanistic aspects on which the final features are grounded. This work shows the temperature induced structural rearrangements of nanocomposites based on poly[ethylene‐co‐(vinyl acetate)] (EVA) intercalated‐organomodified clay (at 3–30 wt.‐% silicate addition) which occur in the range between 75 and 350 °C. In situ high temperature X‐ray diffraction (HT‐XRD) studies have been performed under both nitrogen and air to monitor the modifications of the nanocomposite structure at increasing temperatures under inert/oxidative atmosphere. Heating between 75 and 225 °C, under nitrogen or air, causes the layered silicate to migrate towards the nanocomposite surface and to increase its interlayer distance. The degradation of both the clay organomodifier and the VA units of the EVA polymer seems to play a key role in driving the evolution of the silicate phase in the low temperature range. The structural modifications of the nanocomposites in the high temperature range (250–350 °C), depended on the atmosphere, either inert or oxidizing, in which the samples were heated. Heating under nitrogen led to deintercalation and thus a decrease of the silicate interlayer space, whereas exfoliation was the main process under air leading to an increase of the silicate interlayer space.
18.
[1-14C]Heptane was aromatized under 5 atm pressure over a “nonacidic” 3% platinum on alumina catalyst maintained at 500 °C. The reactant was pumped in at a HLSV of 0.88, and the hydrogen-to-heptane mole ratio was 5.1. The samples of product were collected (one during the first 90 min and another during the next 90 min), the toluene was separated from the respective samples, and the radioactivity in each position of the toluene was determined by a degradation sequence. The distribution of radioactivity in sample 1 was as follows: in position 1–2.6%, 2–38.6%; 3–14.5%; 4–4.2%, and in the methyl group—40.1%. Approximately similar distribution of radioactivity was found in sample 2. 1,6-Ring closure is the most important pathway leading to the formation of toluene from [1-14C]heptane; lesser amounts of repetitive, 1,5-ring closure and hydrogenolysis, and bond shift reactions followed by 1,6-ring closure and dehydration can explain the distribution of radioactivity. The participation of 1,7-ring closure in the reaction is also indicated. 相似文献
19.
Finkbeiner Bernd Mariani Leonardo 《International Journal on Software Tools for Technology Transfer (STTT)》2021,23(4):545-546
International Journal on Software Tools for Technology Transfer - 相似文献
20.
Aniello Leonardo Halak Basel Chai Peter Dhall Riddhi Mihalea Mircea Wilczynski Adrian 《International Journal of Information Security》2021,20(3):445-460
International Journal of Information Security - The complexity of today’s integrated circuit (IC) supply chain, organised in several tiers and including many companies located in different... 相似文献