Many lines of evidence have suggested that angiotensin II (AngII) plays an important role in the development of cardiac hypertrophy through AngII type 1 receptor (AT1). To determine whether AngII is indispensable for the development of mechanical stress-induced cardiac hypertrophy, we examined the activity of mitogen-activated protein kinase (MAPK) family and the expression of the c-fos gene as hypertrophic responses after stretching cultured cardiac myocytes of AT1a knockout (KO) mice. When cardiac myocytes were stretched by 20% for 10 min, extracellular signal-regulated protein kinases (ERKs) were strongly activated in KO cardiomyocytes as well as wild type (WT) myocytes. Both basal and stimulated levels of ERKs were higher in cardiomyocytes of KO mice than in those of WT mice. Activation of another member of the MAPK family, p38(MAPK), and expression of the c-fos gene were also induced by stretching cardiac myocytes of both types of mice. An AT1 antagonist attenuated stretch-induced activation of ERKs in WT cardiomyocytes but not in KO cardiomyocytes. Down-regulation of protein kinase C inhibited stretch-induced ERK activation in WT cardiomyocytes, whereas a broad spectrum tyrosine kinase inhibitor (genistein) and selective inhibitors of epidermal growth factor receptor (tyrphostin, AG1478, and B42) suppressed stretch-induced activation of ERKs in KO cardiac myocytes. Epidermal growth factor receptor was phosphorylated at tyrosine residues by stretching cardiac myocytes of KO mice. These results suggest that mechanical stretch could evoke hypertrophic responses in cardiac myocytes that lack the AT1 signaling pathway possibly through tyrosine kinase activation. 相似文献
We propose a novel online multiple object tracker taking structure information into account. State-of-the-art multi-object tracking (MOT) approaches commonly focus on discriminative appearance features, while neglect in different levels structure information and the core of data association. Addressing this, we design a new tracker fully exploiting structure information and encoding such information into the cost function of the graph matching model. Firstly, a new measurement is proposed to compare the structure similarity of two graphs whose nodes are equal. With this measurement, we define a complete matching which performs association in high efficiency. Secondly, for incomplete matching scenarios, a structure keeper net (SKnet) is designed to adaptively establish the graph for matching. Finally, we conduct extensive experiments on benchmarks including MOT2015 and MOT17. The results demonstrate the competitiveness and practicability of our tracker.
针对推荐系统中用户评分数据稀疏所导致推荐结果不精确的问题,本文尝试将用户评分、信任关系和项目评论文本信息融合在概率矩阵分解方法中以缓解评分数据稀疏性问题.首先以共同好友数目及项目流行度改进皮尔逊用户偏好相似程度并将其作为用户间的直接信任值,然后考虑用户间信任传播过程中所有路径的影响构建新的信任网络;其次通过BERT预训练(Pre-training of Deep Bidirectional Transformers for Language Understanding)模型提取项目的评论文本向量,构造项目的评论文本特征矩阵;最后基于概率矩阵分解(Probabilistic Matrix Factorization,PMF)模型融合用户的评分数据、用户的信任关系以及项目的评论文本信息进行推荐.通过不断的理论分析并在真实的Yelp数据集上进行实验验证,均表明本文算法的有效性. 相似文献
In this article, the kernel-based methods explained by a graph embedding framework are analyzed and their nature is revealed,
i.e. any kernel-based method in a graph embedding framework is equivalent to kernel principal component analysis plus its
corresponding linear one. Based on this result, the authors propose a complete kernel-based algorithms framework. Any algorithm
in our framework makes full use of two kinds of discriminant information, irregular and regular. The proposed algorithms framework
is tested and evaluated using the ORL, Yale and FERET face databases. The experiment results demonstrate the effectiveness
of our proposed algorithms framework. 相似文献
The objective of this study is to develop a tri-rotor flying robot, which adopts the Y-shaped three-rotor structure. In order to balance the yaw torque produced by the three rotors, the RC servomotor and linkage is installed on the tail axis in order to improve the angle of the rolling axis of the tail motor. Moreover, through the torque generated by the horizontal component of the lift from the inclined motor on the tail axis, it balances the yaw torque of the three rotors. The dynamic equations of the tri-rotor flying robot are determined in this paper. The relationship between motor thrust, angular acceleration, and voltage input was also studied in this research. In order to study the effect of control parameters on the flight stability completely, this study develops a universal stability experimental platform to help tuning the control parameters safely. Based on this, the tri-rotor flying robot can rapidly change flying gesture and avoid oscillation. Finally, we conducted some indoor and outdoor flight tests. From the experimental results, the tri-rotor flying robot can fly and hover stably in the sky. 相似文献