Egg Thiamine Status of Lake Ontario Salmonines 1995–2004 with Emphasis on Lake Trout

Alewives (Alosa pseudoharengus), the major prey fish for Lake Ontario, contain thiaminase. They are associated with development of a thiamine deficiency in salmonines which greatly increases the potential for developing an early mortality syndrome (EMS). To assess the possible effects of thiamine deficiency on salmonine reproduction we measured egg thiamine concentrations for five species of Lake Ontario salmonines. From this we estimated the proportion of families susceptible to EMS based on whether they were below the ED20, the egg thiamine concentration associated with 20% mortality due to EMS. The ED20s were 1.52, 2.63, and 2.99 nmol/g egg for Chinook salmon (Oncorhynchus tshawytscha), lake trout (Salvelinus namaycush), and coho salmon (Oncorhynchus kisutch), respectively. Based on the proportion of fish having egg thiamine concentrations falling below the ED20, the risk of developing EMS in Lake Ontario was highest for lake trout, followed by coho (O. kisutch), and Chinook salmon, with the least risk for rainbow trout (O. mykiss). For lake trout from western Lake Ontario, mean egg thiamine concentration showed significant annual variability during 1994 to 2003, when the proportion of lake trout at risk of developing EMS based on ED20 ranged between 77 and 100%. Variation in the annual mean egg thiamine concentration for western Lake Ontario lake trout was positively related (p < 0.001, r² = 0.94) with indices of annual adult alewife biomass. While suggesting the possible involvement of density-dependent changes in alewives, the changes are small relative to egg thiamine concentrations when alewife are not part of the diet and are of insufficient magnitude to allow for natural reproduction by lake trout.     

The extrusion of polymers below their melting temperatures by the application of high pressures

A number of polymers have been “cold” extruded (i.e. at temperatures well below their normal melting temperatures) by the application of relatively high pressures. In all cases extrudates of relatively large cross-sectional area have been produced, and non-circular cross-sections have also been extruded. The extruded products has been evaluated and the results discussed. In general it has been found that whilst extrusion gives an oriented product, the increase in tensile modulus is much less than for a comparably drawn fibre. This phenomenon has been discussed in terms of the changes in crystalline structure produced by extrusion.     

Supported Alumina Members by an in-Situ Sol–Gel Method

Alumina membranes were prepared using a sol–gel process. Thin gel layers were grown onto porous alumina support tubes by using the permeation of water through the tube to control the rate of hydrolysis. Optimum coating conditions were found to be an ASB to 2-propanol volume ratio of 1:25, coating times between 10 and 15 s, and partial drying times between 2.5 and 3 min, depending on whether the water was flowing in the tube or stored in the tube. The coatings remained crack-free and adherent when fired to 800°C.     

高强聚烯烃静态断裂过程银纹区的性能1.HDPE

为了观察分析高密度聚乙烯(HDPE)的银纹区性能,对两种HDPE全切痕试样分别进行了速率为0.1 mm/min,0.5 mm/min,1 mm/min,5 mm/min,20 mm/min和50 mm/min的拉伸测试。基于对静态断裂过程应力-位移数据的分析处理,结果表明,HDPE的屈服应力随着拉伸速率增大;高应力时两种HDPE均存在常临界位移控制其银纹断裂;HDPE2480银纹微纤的稳定性较大,材料韧性较好。HDPE静态拉伸断面显微结构显示,HDPE2480的银纹微纤尺寸大于HDPE4801,导致两者断面弧形巢尺寸和弧形巢内粗糙度的不同。     

SARS-CoV-2 Infection Dysregulates Cilia and Basal Cell Homeostasis in the Respiratory Epithelium of Hamsters

Similar to many other respiratory viruses, SARS-CoV-2 targets the ciliated cells of the respiratory epithelium and compromises mucociliary clearance, thereby facilitating spread to the lungs and paving the way for secondary infections. A detailed understanding of mechanism involved in ciliary loss and subsequent regeneration is crucial to assess the possible long-term consequences of COVID-19. The aim of this study was to characterize the sequence of histological and ultrastructural changes observed in the ciliated epithelium during and after SARS-CoV-2 infection in the golden Syrian hamster model. We show that acute infection induces a severe, transient loss of cilia, which is, at least in part, caused by cilia internalization. Internalized cilia colocalize with membrane invaginations, facilitating virus entry into the cell. Infection also results in a progressive decline in cells expressing the regulator of ciliogenesis FOXJ1, which persists beyond virus clearance and the termination of inflammatory changes. Ciliary loss triggers the mobilization of p73⁺ and CK14⁺ basal cells, which ceases after regeneration of the cilia. Although ciliation is restored after two weeks despite the lack of FOXJ1, an increased frequency of cilia with ultrastructural alterations indicative of secondary ciliary dyskinesia is observed. In summary, the work provides new insights into SARS-CoV-2 pathogenesis and expands our understanding of virally induced damage to defense mechanisms in the conducting airways.     

通过仿真确保信号完整性设计

PowerPC处理器能帮助实现快速灵活的可编程嵌入式平台设计.Virtex-4 FX平台利用集成于双PowerPC处理器的创新辅助处理器单元(APU)控制器,支持新的硬件加速应用.FX APU控制器又大大改善了软件算法的执行,提高了FPGA资源可用性.本文将介绍使用Xilinx/Ansoft 10Gbps背板设计套件确保互连性能.