Isolation and characterization of C-reactive protein (CRP) cDNA and genomic DNA from Xenopus laevis. A species representing an intermediate stage in CRP evolution

C-reactive protein (CRP) is a prototypic acute phase protein in human and rabbit. Although it is structurally and functionally conserved from invertebrate to human, there are species-specific differences in patterns of expression and putative function. To further investigate the biological significance, regulation, and evolution of CRP, we isolated Xenopus CRP and subsequently derived and sequenced corresponding cDNA and the genomic clones. The structure and expression of Xenopus CRP were also compared to those of the other CRPs. Analyses of the amino acid sequence and the nucleotide sequence reveal that the mature Xenopus CRP is a 222-amino acid protein preceded by a 16-residue signal peptide. During development, Xenopus CRP is expressed, only when the liver appears, and therefore is not likely to play a role in early embryonic development. Compared to other species, Xenopus CRP is present at an intermediate low level of < 1 microgram/ml in the normal serum. Unlike human and rabbit CRP, Xenopus CRP is not induced by turpentine or heatshock treatment. The heatshock consensus sequence (Woo, P., Korenberg, J. R., and Whitehead, A. S. (1985) J. Biol. Chem. 265, 4136-4142) are not present in the Xenopus CRP gene. It is suggested that Xenopus CRP represents a transitional period in CRP evolution when host defenses switched from primitive innate immunity to a much more complex immune system. The constitutive functions of CRP gradually became less essential as the result of the development of a complex immune system.     

Determination of complements in serum and middle ear effusion of the patients with secretory otitis media

Complements in serum and middle ear effusion were determined in 20 patients with secretory otitis media, and compared with those in the normal controls. The C5 and C1-INH in serum of the patients were significantly higher. On the contrary, C9 and B factor (Bf) were significantly lower, and the circulatory immunocomplex was also higher. In patients with secretory otitis media, the middle ear effusion levels of C3, C4 and C5 were significantly lower, and Bf and immunocomplex were significantly higher than those in serum. The results suggest that the ability of complements in clearing immunocomplex is low. Therefore, the immunocomplex may deposit in the mucosa of the middle ear. Thus the permeability of capillary will be increased, and the middle ear effusion occurs.     

大型离心复合球墨铸铁轧辊的生产

简述了我公司为舞阳铜厂试制４２００ｍｍ厚板轧机离心复合球墨铸铁轧辊的性能要求、内外层双重金属铸造工艺参数的选择及整个铸造生产工艺过程。     

HDPE装置超负荷低能耗生产方案的设计与实施

本文介绍大庆ＨＤＰＥ装置提高生产能力及产品质量的方法。通过采用釜外络合催化剂新技术和提浆液浓度可将ＨＤＰＥ装置聚合釜单釜的生产能力由４．７５ｔ／ｈ提高到５．０ｔ／ｈ，各项能耗下降，收到了增产降耗的显著效果，并使产品质量，产量，品种，能耗，物耗，环保６项指标全部达到了设计要求。     

传爆管用聚奥99炸药研究

介绍了具有高煤轰感度和耐热特性的聚奥99炸药的配方、制备工艺、性能以及装入传爆管后产品的性能和应用状况。     

Novel polymeric aromatic lithium sulfonylimides as salts for polymer electrolytes

Three new polymeric aromatic lithium sulfonylimides were used as salts in high molecular weight poly(ethylene oxide) (PEO) electrolytes. Their conductivity and electrochemical stability behaviors were investigated. The electrolytes with lithium poly[4,4′‐(hexafluoroisopropylidene)diphenoxy]sulfonylimide (LiPHFIPSI) showed a better conductive performance compared with the other two lithium salts. The best conductivity was obtained for PEO/LiPHFIPSI EO/Li = 16 (1.90 × 10^?4 S/cm at 60°C). Thermal analysis indicated that the salts effectively decreased the crystallinity of PEO. Moreover, the electrolytes also had good electrochemical stability and their oxidative potential was to 5.5 V versus Li/Li⁺. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 85: 1802–1805, 2002     

压制球形药包解决退模问题的技术措施

文中简要阐述了在制备球形药包过程中,为解决压装药件的退模问题所采取的技术措施,并提出了较佳的成型底座的设计方案。     

高分子量阴离子型聚丙烯酰胺的制备

介绍了聚丙烯酰胺的品种、性能及用途；研究了以丙烯酸为共聚单体，采用反相乳液聚合制备高分子量阴离子型聚丙烯酰胺胶乳的原理和方法；分析讨论了原料纯度、乳化剂和引发剂种类及操作条件等因素对产品性能的影响．     

Reconstruction of cervical stability following old facet dislocation of cervical spine]

One hundred fifty adolescent inpatients with major depression were systematically assessed for demographic and clinical differences between psychotic and nonpsychotic depression. Delusions and/or hallucinations were present in 10% of the subjects. The psychotic group had significantly more frequent and severe suicidal ideation. Posttraumatic stress disorder was also more frequent in the psychotic group.