Myoglobin/gold nanoparticles/carbon spheres 3-D architecture for the fabrication of a novel biosensor

A novel biosensor based on a myoglobin/gold nanoparticles/carbon spheres (Mb-AuNPs-CNs) 3-D architecture bioconjunction has been fabricated for the determination of hydrogen peroxide (H₂O₂). Cyclic voltammetry (CV), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) were used to characterize the bioconjunction of the AuNPs-CNs with Mb. Experimental results demonstrate that the AuNPs-CNs hybrid material is more effective in facilitating electron transfer of the immobilized enzyme than CNs alone, which can be attributed to the unique nanostructure and larger surface area of the bioconjunction. The biosensor displayed good performance for the detection of H₂O₂ with a wide linear range from 0.28 μmol/L to 116.5 μmol/L and a detection limit of 0.12 μmol/L. The Michaelis-Menten constant K _M^app value was estimated to be 0.3 mmol/L. The resulting biosensor exhibited fast amperometric response, and good stability, reproducibility, and selectivity to H₂O₂. This article is published with open access at Springerlink.com     

一种实用的精密测量设备空气弹簧监控系统

介绍了典型空气弹簧系统的组成和原理.以此为基础,根据几何量精密测量的需要,研制了一种电子与机械相结合、安全可靠的空气弹簧自动监控系统.系统的关键部分包括气动升高安全控制机构和平台调平机构,基于计算机平台传感系统,信号分析处理和监测系统.该监控系统可直接用于大型高精度几何量测量设备(如激光干涉比长仪),实现仪器振动的隔离、自动调平、高度控制等功能.     

用磁控溅射法制备的Ag-MgF2的复合薄膜的研究

采用RF磁控溅射方法制备了Ag-MgF2复合薄膜.通过XRD测量,发现当退火温度在300～500℃时,Ag以晶态形式镶嵌在非晶态介质中;当退火温度低于300℃时候,Ag以非晶态形式存在.在分层镀Ag和MgF2薄膜后,经过热处理使Ag颗粒扩散在MgF2介质中,通过AFM观察,对复合薄膜形貌进行了分析:如果先镀MgF2薄膜,再镀Ag薄膜,发现Ag颗粒主要分布在薄膜的表面,并且富集形成较大的颗粒,表现出块体Ag的吸收特性.而先镀Ag薄膜,然后再镀MgF2薄膜,经过热处理,可以形成Ag颗粒均匀镶嵌在介质中形成的复合薄膜.同时对样品的吸收特性也进行了研究.     

In-fiber liquid-level probe based on Michelson interferometer via dual-mode elliptical multilayer-core fiber

Abstract

An in-fiber liquid-level probe fabricated from homemade dual-mode elliptical multilayer-core fiber (EMCF) was proposed and experimentally demonstrated. The proposed sensor simply consists of a segment of the EMCF with one end coated with silver film, and a Michelson interferometer is roughly established when light from single-mode fiber incident from the other end. The detected interference patterns, rather clean due to the few-mode property, shift as the liquid level due to strong interaction between high-order modes and measurands through evanescent waves. Both the propagation characteristics and operation principle of such a sensor were demonstrated in detail, and sensitivities of 33.48, 43.35, and 48.93 pm/mm corresponding to liquid indices of 1.333, 1.353, and 1.373 were successfully achieved with a 50-mm EMCF probe, respectively. Moreover, the proposed sensor had the potential to discriminate measurand index after proper calibration.     

炮孔计算机辅助设计系统研究

对爆破设计中的需求进行了系统分析,确定了炮孔参数和炮孔设计图的形成思想,介绍了炮孔计算机辅助设计中的数据库技术,以及如何利用Arx2000对AutoCAD进行二次开发实现设计功能。     

一种微型输液泵的硬件设计

介绍了一种微型输液泵的硬件设计思想和电路原理 ,并对电路中的一些关键器件作了介绍。     

利用数值模拟技术对锦310块开发方案进行优选

锦310断块油藏为新增探明储量区块,属深层、低孔、低渗复杂油藏,层间及层内矛盾突出,目前国内外对该类油藏的开发都存在一定的难度.为了更有效地控制储量及合理地进行开发设计,在开展油藏工程研究的基础上,利用油藏数值模拟技术,对各种可行的开发方案进行预测和优选,探讨低渗复杂断块油藏水平井与直井组合开采的数值模拟,取得了较好的预测结果,对实际井网部署提供了理论根据.