Human C-peptide. Part I: Radioimmunoassay

Synthetic human C-peptide bearing a Tyrosine group at its amino end is labelled with 125iodine using chloramin T or hydrogen peroxide and lactoperoxidase. The results are compared applying both methods. Antiserum to synthetic human C-peptide (without Tyrosine) which was partially compared to rabbit albumin, is raised in guinea pigs and goats. Goats show to be superior to guinea pigs concerning antibody production. The so-called "hook effect" phenomenon is observed in setting up the standard curves for the radioimmunoassay. Monotonically decreasing standard curves are obtained on dilution of antiserum with a high antibody titer which was produced by repeated immunization in goats. Free C-peptide and C-peptide bound to antiserum are separated with the anxion exchange resin Amberlite. Using this separation technique we excluded unspecific binding of labelled C-peptide to protein fractions in serum of diabetics. The sensitivity of our radioimmunoassay is approx. 0.3 ng C-peptide/ml serum. Intra- and interassay variability are below 10%. Human proinsulin is the only substance found to crossreact with the antiserum.     

Induction of rabbit renal mixed-function oxidases by phenobarbital: cell specific ultrastructural changes in the proximal tubule

Administration of phenobarbital (60 mg/kg) daily for 4 days to male rabbits resulted in induction of renal cytochrome P-450 (3.5-fold) and a corresponding increase in ethoxycoumarin-O-deethylase and benzphetamine-N-demethylase activity (17- and 4-fold, respectively). Kidney weight to body weight ratio and renal ethoxyresorufin-O-deethylase were not affected by phenobarbital pretreatment. Numerous focal areas of proliferation of smooth endoplasmic reticulum (SER) were evident in proximal tubule cells from phenobarbital treated rabbits while proximal tubular cells from control rabbits had only small and sparcely located aggregates of SER. Phenobarbital-induced SER proliferation was specifically localized to the S3 segment of the proximal tubule. Proliferation was not observed in S2 cells of the proximal tubule, cells of Henle's loop, distal tubules, or collecting tubules in rabbits pretreated with phenobarbital. These data demonstrate the biochemical heterogeneity of cell types within the proximal tubules of rabbits. Furthermore, induction of mixed-function oxidases specifically in S3 cells of the proximal tubule may be of toxicological significance in the metabolic activation of certain nephrotoxicants.     

Use of neural networks for predictions using time series: Illustration with the El Niño Southern oscillation phenomenon

This paper introduces the use of sets of multiple networks (bundled networks) to manage the variability due to different initialization parameters. This method makes it statistically impossible for the networks to be trapped in the same local minimum, and therefore allows better control of the confidence of the prediction eventually given. The spread of the forecasts given by these different networks can be used for prediction reliability purposes. An illustration of this usage is given with the El Niño phenomenon.     

Efficient calculation of intrinsic low-dimensional manifolds for the simplification of chemical kinetics

During the last years the interest in the numerical simulation of reacting flows has grown considerably and numerical methods are available, which allow to couple chemical kinetics with flow and molecular transport. The use of detailed physical and chemical models, involving several hundred species, is restricted to very simple flow configurations like one-dimensional systems or two-dimensional systems with very simple geometries, and models are required, which simplify chemistry without sacrificing accuracy. One method to simplify the chemical kinetics is based on Intrinsic Low-Dimensional Manifolds (ILDM). They represent attractors for the chemical kinetics, i.e. fast chemical processes relax towards them, and slow chemical processes represent movements within the manifolds. Thus the identification of the ILDMs allows a decoupling of the fast time scales. The concept has been verified by many different reacting flow calculations. However, one remaining problem of the method is the efficient calculation of the low-dimensional manifolds. This problem is addressed in this paper. We present an efficient, robust method, which allows to calculate intrinsic low-dimensional manifolds of chemical reaction systems. It is based on a multi-dimensional continuation process. Examples are shown for a typical combustion system. The method is not restricted to this problem class, but can be applied to other reacting flows or dynamic systems provided that a large number of decaying components can be eliminated from the system. Received: 5 May 1996 / Accepted: 2 September 1997     

A stereophonic acoustic signal extraction scheme for noisy and reverberant environments

In this contribution, a novel two-channel acoustic front-end for robust automatic speech recognition in adverse acoustic environments with nonstationary interference and reverberation is proposed. From a MISO system perspective, a statistically optimum source signal extraction scheme based on the multichannel Wiener filter (MWF) is discussed for application in noisy and underdetermined scenarios. For free-field and diffuse noise conditions, this optimum scheme reduces to a Delay & Sum beamformer followed by a single-channel Wiener postfilter. Scenarios with multiple simultaneously interfering sources and background noise are usually modeled by a diffuse noise field. However, in reality, the free-field assumption is very weak because of the reverberant nature of acoustic environments. Therefore, we propose to estimate this simplified MWF solution in each frequency bin separately to cope with reverberation. We show that this approach can very efficiently be realized by the combination of a blocking matrix based on semi-blind source separation (‘directional BSS’), which provides a continuously updated reference of all undesired noise and interference components separated from the desired source and its reflections, and a single-channel Wiener postfilter. Moreover, it is shown, how the obtained reference signal of all undesired components can efficiently be used to realize the Wiener postfilter, and at the same time, generalizes well-known postfilter realizations. The proposed front-end and its integration into an automatic speech recognition (ASR) system are analyzed and evaluated in noisy living-room-like environments according to the PASCAL CHiME challenge. A comparison to a simplified front-end based on a free-field assumption shows that the introduced system substantially improves the speech quality and the recognition performance under the considered adverse conditions.     

Electronic diary evidence on energy erosion in clinical burnout.

Burnout is generally defined as a state of severe exhaustion. So far, research has predominantly focused on relatively mild burnout in employees able to work despite their complaints. This study examines energy depletion in clinical burnout (e.g., the severest cases on extended sick leave) by comparing the diurnal patterns of fatigue and exhaustion with those of healthy individuals. Sixty clinically burned-out and 40 healthy participants kept an electronic diary for 14 days, 7 times a day, yielding a total of 8,116 diary entries. This study shows that burned-out individuals typically suffer continuously from a severe fatigue throughout the day. The resulting flattened diurnal cycles mark a stable exhaustion that is uncommon in healthy persons. The current results provide novel support for the existence of severe energy erosion in clinical burnout. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Computer adaptive practice of Maths ability using a new item response model for on the fly ability and difficulty estimation

In this paper we present a model for computerized adaptive practice and monitoring. This model is used in the Maths Garden, a web-based monitoring system, which includes a challenging web environment for children to practice arithmetic. Using a new item response model based on the Elo (1978) rating system and an explicit scoring rule, estimates of the ability of persons and the difficulty of items are updated with every answered item, allowing for on the fly item calibration. In the scoring rule both accuracy and response time are accounted for. Items are sampled with a mean success probability of .75, making the tasks challenging yet not too difficult. In a period of ten months our sample of 3648 children completed over 3.5 million arithmetic problems. The children completed about 33% of these problems outside school hours. Results show better measurement precision, high validity and reliability, high pupil satisfaction, and many interesting options for monitoring progress, diagnosing errors and analyzing development.