Eicosapentaenoic and docosahexaenoic acid affect mitochondrial and peroxisomal fatty acid oxidation in relation to substrate preference

Decreased triacylglycerol synthesis within hepatocytes due to decreased diacylglycerol acyltransferase (DGAT) activity has been suggested to be an important mechanism by which diets rich in fish oil lower plasma triacylglycerol levels. New findings suggest that eicosapentaenoic acid (EPA), and not docosahexaenoic acid (DHA), lowers plasma triacylglycerol by increased mitochondrial fatty acid oxidation and decreased availability of fatty acids for triacylglycerol synthesis. To contribute to the understanding of the triacylglycerol-lowering mechanism of fish oil, the different metabolic properties of EPA and DHA were studied in rat liver parenchymal cells and isolated rat liver organelles. EPA-CoA was a poorer substrate than DHA-CoA for DGAT in isolated rat liver microsomes, and in the presence of EPA, a markedly lower value for the triacyl[³H]glycerol/diacyl[³H]glycerol ratio was observed. The distribution of [1-¹⁴C]palmitic acid was shifted from incorporation into secreted glycerolipids toward oxidation in the presence of EPA (but not DHA) in rat liver parenchymal cells. [1-¹⁴C]EPA was oxidized to a much greater extent than [1-¹⁴C]DHA in rat liver parenchymal cells, isolated peroxisomes, and especially in purified mitochondria. As the oxidation of EPA was more effective and sensitive to the CPT-I inhibitor, etomoxir, when measured in a combination of both mitochondria and peroxisomes, we hypothesized that both are involved in EPA oxidation, whereas DHA mainly is oxidized in peroxisomes. In rats, EPA treatment lowered plasma triacylglycerol and increased hepatic mitochondrial fatty acid oxidation and carnitine palmitoyltransferase (CPT)-I activity in both the presence and absence of malonyl-CoA. Whereas only EPA treatment increased the mRNA levels of CPT-I, DHA treatment increased the mRNA levels of peroxisomal fatty acyl-CoA oxidase and fatty acid binding protein more effectively than EPA treatment. In conclusion, EPA and DHA affect cellular organelles in relation to their substrate preference. The present study strongly supports the hypothesis that EPA, and not DHA, lowers plasma triacylglycerol by increased mitochondrial fatty acid oxidation.     

Non‐parametric probabilistic forecasts of wind power: required properties and evaluation

Predictions of wind power production for horizons up to 48–72 h ahead comprise a highly valuable input to the methods for the daily management or trading of wind generation. Today, users of wind power predictions are not only provided with point predictions, which are estimates of the conditional expectation of the wind generation for each look‐ahead time, but also with uncertainty estimates given by probabilistic forecasts. In order to avoid assumptions on the shape of predictive distributions, these probabilistic predictions are produced from non‐parametric methods, and then take the form of a single or a set of quantile forecasts. The required and desirable properties of such probabilistic forecasts are defined and a framework for their evaluation is proposed. This framework is applied for evaluating the quality of two statistical methods producing full predictive distributions from point predictions of wind power. These distributions are defined by a number of quantile forecasts with nominal proportions spanning the unit interval. The relevance and interest of the introduced evaluation framework are discussed. Copyright © 2007 John Wiley & Sons, Ltd.     

Short communication: use of culling information in genetic evaluation of fertility and mastitis in Norwegian red cows

Breeding values for clinical mastitis, interval from calving to first insemination, and 56-d nonreturn rate for heifers and primiparous cows, were predicted using multivariate linear-threshold sire models, with or without including information on culling during the first lactation. Breeding values for 3,064 sires were predicted using 3 data sets with an average of 273, 135, and 68 first-crop daughters per sire, respectively. For each data set, accuracies of selection for health and fertility traits were evaluated through the predictive ability of predicted sire breeding values with respect to phenotypic performance of second-crop daughters. The predictive ability of estimated breeding values for clinical mastitis and interval from calving to first insemination did not improve when including information on early culling, irrespective of the size of first-crop daughter groups. For 56-d nonreturn rates (heifer and primiparous cow), sire evaluations based on reduced size of daughter groups tended to predict performance of the future daughters slightly better when including data on early culling. Hence, for breeding programs with direct selection for health and fertility traits there is little to gain by including early culling as additional information.     

Spatio‐temporal analysis and modeling of short‐term wind power forecast errors

Forecasts of wind power production are increasingly being used in various management tasks. So far, such forecasts and related uncertainty information have usually been generated individually for a given site of interest (either a wind farm or a group of wind farms), without properly accounting for the spatio‐temporal dependencies observed in the wind generation field. However, it is intuitively expected that, owing to the inertia of meteorological forecasting systems, a forecast error made at a given point in space and time will be related to forecast errors at other points in space in the following period. The existence of such underlying correlation patterns is demonstrated and analyzed in this paper, considering the case‐study of western Denmark. The effects of prevailing wind speed and direction on autocorrelation and cross‐correlation patterns are thoroughly described. For a flat terrain region of small size like western Denmark, significant correlation between the various zones is observed for time delays up to 5 h. Wind direction is shown to play a crucial role, while the effect of wind speed is more complex. Nonlinear models permitting capture of the interdependence structure of wind power forecast errors are proposed, and their ability to mimic this structure is discussed. The best performing model is shown to explain 54% of the variations of the forecast errors observed for the individual forecasts used today. Even though focus is on 1‐h‐ahead forecast errors and on western Denmark only, the methodology proposed may be similarly tested on the cases of further look‐ahead times, larger areas, or more complex topographies. Such generalization may not be straightforward. While the results presented here comprise a first step only, the revealed error propagation principles may be seen as a basis for future related work. Copyright © 2010 John Wiley & Sons, Ltd.     

Fouling Formation During Hydrogen Sulfide Scavenging With 1,3,5-tri-(hydroxyethyl)-hexahydro-s-triazine

To investigate if the hydrogen sulfide scavenger, 1,3,5-tri-(hydroxyethyl)-hexahydro-s-triazine, could explain a severe carbon/sulfur rich fouling found at a refinery, experiments were conducted to study the scavenging process and the composition of the fouling. The reaction was analyzed with electro spray ionization mass spectrometry (ESI-MS), and the fouling with infrared spectroscopy and X-ray diffraction. The fouling was found to be a by-product of the scavenging reaction, and to consist of dithiazine molecules with linking carbon-sulfur chains. ESI-MS analysis strongly indicates that these chains are generated when dithiazine decomposes to smaller molecules, which through condensation reactions with dithiazine forms the fouling.     

Antioxidative activity of summer savory (Satureja hortensis L.) and rosemary (Rosmarinus officinalis L.) in minced, cooked pork meat

The sensory intensity measured as theabsolute threshold value of spice andrecognition of spice was evaluated for summer savory and rosemary in meat balls. The values forabsolute threshold value of spice were found to be significantly lower for rosemary than for summer savory. The antioxidative activity of the spices was analysed in two accelerated model systems and in a storage experiment. In one of the model systems, oxidation was accelerated by heat and by an elevated pressure of oxygen. Both spices significantly improved the oxidative stability of the meat balls in this model system. In the second model system metal catalyst oxidation in a meat slurry was used and a reduction in the oxidative processes in samples containing the spices was again shown. In both model systems a slightly higher antioxidative activity was seen for rosemary compared to summer savory. In the storage experiment, the spices were added at a sensorially acceptable level and the heat-treated meat balls were stored at 5°C. A significant reduction in the development of warmed-over flavour (WOF) caused by the addition of spices was measured by a reduction in 2-thiobarbituricacid-reactivesubstances (TBARS) and in hexanal. A reduction in TBARS of approximately 30% in meat balls containing added spices was seen independently of packaging in 1% oxygen or in atmospheric air and the reduction (30%) was constant throughout the storage period.     

Ceramic collaborations around the world

International collaboration can be an integral part of a research project. It can add scientific expertise or interdisciplinarity, enhance equipment and facilities; and strengthen the training and education of students. In recognition of these benefits, the National Science Foundation (NSF) of the United States has supported researchers in many different modes. One option for ceramists is the Materials World Network (MWN) program. Today, approximately 24% of the active projects in the Ceramics Program (CER) of NSF’s Division of Materials Research (DMR) are funded through the MWN program (or its precursors). Each project includes participants from two or more countries and collectively they include researchers from 16 countries. This year an additional 33 ceramic proposals, including renewals, were merit-reviewed in panels and seven awards were made. Characteristics of the best proposals include synergistic pairings of interests and expertise between international partners, detailed plans for student exchanges, activities to broaden the participation of groups under-represented in science, and cutting-edge creative and original research ideas. As well, NSF supports many international activities through the Office of International Science and Engineering (OISE) including regional opportunities. Instrumentation and facilities supported by NSF, particularly those in Materials Science and Engineering, are often accessible on an international basis.     

Plant fibre composites – porosity and stiffness

Plant fibre composites contain typically a relatively large amount of porosity which influences their performance. A model, based on a modified rule of mixtures, is presented to include the influence of porosity on the composite stiffness. The model integrates the volumetric composition of the composites with their mechanical properties. The fibre weight fraction is used as an independent parameter to calculate the complete volumetric composition. A maximum obtainable stiffness of the composites is calculated at a certain transition fibre weight fraction, which is characterised by a best possible combination of high fibre volume fraction and low porosity. The model is validated with experimental data from the literature on several types of composites. A stiffness diagram is presented to demonstrate that the calculations can be used for tailoring and design of composites with a given profile of properties.