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31.
Although it is a hazardous adventure to define what is an ideal in any field, it would appear that there is a certain consensus about total hip prostheses. The fundamental principle of the total hip replacement was based on the articulation of a spheric segment, a mobile head contained in a hemispheric non-retaining element. To date, the first material is a titanium in a alloy containing 6% aluminum and 4% vanadium. The second material facing the titanium is polyethylene or alumine. Thus the joint couple could be "polyethylene-metal", or "polyethylene-alumine" or even "alumine-alumine". The hemispheric element can be a massive piece of polyethylene or formed by a peripheral metal (an open hemisphere) filled with the joint surface, itself made of polyethylene. This solution offers the possibility of changing the prosthesis in case of wear without interrupting the implant-bone contact. The first type is cemented to the bone with methyl polymethacrylate. With the second, the metallic part need not to be cemented and can be fitted to the bone by simple pressing. Several points are essential in the femoral piece. It should include a removable head so the length of the neck can be adjusted. It must fill the medullary canal as closely as possible and fill the methaphyseal space. It is fixed by a self-fitting system leading to stable secondary long-term fixation-a porous covering, sometimes with hydroxy apatite can also be useful. In certain cases it must be cemented, although the cement must only play the role of adaptation.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
32.
Mikael Goldstein Didier Chincholle Mårten Backström 《Personal and Ubiquitous Computing》2000,4(2-3):123-133
Text and digit entry speed of two wearable one-handed input paradigms, the Finger-Joint-Gesture palm-keypad glove and the Invisible Phone Clock, were benchmarked against traditional one-handed cellular phone keypad input in a repeated-measurement design employing 18 subjects using a 9-word sentence. No significant difference in error-corrected text entry speed (5.3 ec-wpm) was found. Digit entry speed was significantly faster (8.3 ec-wpm) and differed significantly between input paradigms. Furthermore, digit entry was fastest for the traditional cellular phone keypad and slowest for the Invisible Clock keypad. A prediction model based on Fitts' law slightly overestimated text entry speed for novice users. Another prediction model, where each movement time between successive keys was corrected for key repeat time for each specific input paradigm, predicted the experimental results more accurately. Thirteen of the subjects ranked the Invisible Phone Clock as 1st choice. The subjects' mapping of the Ericsson cellular phone functions (YES, CLR, NO, <,> and Address Book) was not according to the designer's model, partly due to functional fixedness. The input paradigms could be suitable candidates for new fragmentised interfaces where wearability is the key issue. 相似文献
33.
We report on the growth mechanism and density control of vertically aligned carbon nanotubes using a triode plasma enhanced chemical vapor deposition system. The deposition reactor was designed in order to allow the intermediate mesh electrode to be biased independently from the ground and power electrodes. The CNTs grown with a mesh bias of + 300 V show a density of ∼ 1.5 μm− 2 and a height of ∼ 5 μm. However, CNTs do not grow when the mesh electrode is biased to − 300 V. The growth of CNTs can be controlled by the mesh electrode bias which in turn controls the plasma density and ion flux on the sample. 相似文献
34.
35.
Spriet C Trinel D Waharte F Deslee D Vandenbunder B Barbillat J Héliot L 《Microscopy research and technique》2007,70(2):85-94
Studies of proteins' interaction in cells by FRET can take benefit from two important photo-physical properties describing fluorescent proteins: fluorescence emission spectrum and fluorescence lifetime. These properties provide specific and complementary information about the tagged proteins and their environment. However, none of them taken individually can completely quantify the involved fluorophore characteristics due to their multiparametric dependency with molecular environment, experimental conditions, and interpretation complexity. A solution to get a better understanding of the biological process implied at the cellular level is to combine the spectral and temporal fluorescence data acquired simultaneously at every cell region under investigation. We present the SLiM-SPRC160, an original temporal/spectral acquisition system for simultaneous lifetime measurements in 16 spectral channels directly attached to the descanned port of a confocal microscope with two-photon excitation. It features improved light throughput, enabling low-level excitation and minimum invasivity in living cells studies. To guarantee a fairly good level of accuracy and reproducibility in the measurements of fluorescence lifetime and spectra on living cells, we propose a rigorous protocol for running experiments with this new equipment that preserves cell viability. The usefulness of SLiM approach for the precise determination of overlapping fluorophores is illustrated with the study of known solutions of rhodamine. Then, we describe reliable FRET experiments in imaging mode realized in living cells using this protocol. We also demonstrate the benefit of localized fluorescence spectrum-lifetime acquisitions for the dynamic study of fluorescent proteins. proteins. 相似文献
36.
Christophe Brun Pierre‐Henri Elchinger Guillaume Nonglaton Cheikh Tidiane‐Diagne Raluca Tiron Aurélie Thuaire Didier Gasparutto Xavier Baillin 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(33)
Metallic conductive nanowires (NWs) with DNA bundle core are achieved, thanks to an original process relying on double‐stranded DNA alignment and physical vapor deposition (PVD) metallization steps involving a silicon substrate. First, bundles of DNA are suspended with a repeatable process between 2 µm high parallel electrodes with separating gaps ranging from 800 nm to 2 µm. The process consists in the drop deposition of a DNA lambda‐phage solution on the electrodes followed by a naturally evaporation step. The deposition process is controlled by the DNA concentration within the buffer solution, the drop volume, and the electrode hydrophobicity. The suspended bundles are finally metallized with various thicknesses of titanium and gold by a PVD e‐beam evaporation process. The achieved NWs have a width ranging from a few nanometers up to 100 nm. The electrical behavior of the achieved 60 and 80 nm width metallic NWs is shown to be Ohmic and their intrinsic resistance is estimated according to different geometrical models of the NW section area. For the 80 nm width NWs, a resistance of about few ohms is established, opening exploration fields for applications in microelectronics. 相似文献
37.
Mohamad Maatouk Ahmad Ibrahim Lucile Pinault Nicholas Armstrong Said Azza Jean-Marc Rolain Fadi Bittar Didier Raoult 《International journal of molecular sciences》2022,23(10)
The increased exploitation of microbial sequencing methods has shed light on the high diversity of new microorganisms named Candidate Phyla Radiation (CPR). CPR are mainly detected via 16S rRNA/metabarcoding analyses or metagenomics and are found to be abundant in all environments and present in different human microbiomes. These microbes, characterized by their symbiotic/epiparasitic lifestyle with bacteria, are directly exposed to competition with other microorganisms sharing the same ecological niche. Recently, a rich repertoire of enzymes with antibiotic resistance activity has been found in CPR genomes by using an in silico adapted screening strategy. This reservoir has shown a high prevalence of putative beta-lactamase-encoding genes. We expressed and purified five putative beta-lactamase sequences having the essential domains and functional motifs from class A and class B beta-lactamase. Their enzymatic activities were tested against various beta-lactam substrates using liquid chromatography-mass spectrometry (LC-MS) and showed some beta-lactamase activity even in the presence of a beta-lactamase inhibitor. In addition, ribonuclease activity was demonstrated against RNA that was not inhibited by sulbactam and EDTA. None of these proteins could degrade single- and double-stranded-DNA. This study is the first to express and test putative CPR beta-lactamase protein sequences in vitro. Our findings highlight that the reduced genomes of CPR members harbor sequences encoding for beta-lactamases known to be multifunction hydrolase enzymes. 相似文献
38.
The classical way of encoding preferences in decision theory is by means of utility or value functions. However agents are not always able to deliver such a function directly. In this paper, we relate three different ways of specifying preferences, namely by means of a set of particular types of constraints on the utility function, by means of an ordered set of prioritized goals expressed by logical propositions, and by means of an ordered set of subsets of possible choices reaching the same level of satisfaction. These different expression modes can be handled in a weighted logical setting, here the one of possibilistic logic. The aggregation of preferences pertaining to different criteria can then be handled by fusing sets of prioritized goals. Apart from a better expressivity, the benefits of a logical representation of preferences are to put them in a suitable format for reasoning purposes, or for modifying them. 相似文献
39.
Didier Dubois 《国际通用系统杂志》2013,42(2):145-167
An important issue in risk analysis is the distinction between epistemic and aleatory uncertainties. In this paper, the use of distinct representation formats for aleatory and epistemic uncertainties is advocated, the latter being modelled by sets of possible values. Modern uncertainty theories based on convex sets of probabilities are known to be instrumental for hybrid representations where aleatory and epistemic components of uncertainty remain distinct. Simple uncertainty representation techniques based on fuzzy intervals and p-boxes are used in practice. This paper outlines a risk analysis methodology from elicitation of knowledge about parameters to decision. It proposes an elicitation methodology where the chosen representation format depends on the nature and the amount of available information. Uncertainty propagation methods then blend Monte Carlo simulation and interval analysis techniques. Nevertheless, results provided by these techniques, often in terms of probability intervals, may be too complex to interpret for a decision-maker and we, therefore, propose to compute a unique indicator of the likelihood of risk, called confidence index. It explicitly accounts for the decision-maker's attitude in the face of ambiguity. This step takes place at the end of the risk analysis process, when no further collection of evidence is possible that might reduce the ambiguity due to epistemic uncertainty. This last feature stands in contrast with the Bayesian methodology, where epistemic uncertainties on input parameters are modelled by single subjective probabilities at the beginning of the risk analysis process. 相似文献
40.
Design challenges of a 65 nm CMOS stacked folded differential PA structure for mobile communications
Yohann Luque Nathalie Deltimple Eric Kerhervé Didier Belot 《Analog Integrated Circuits and Signal Processing》2011,66(2):155-162
In this paper, a novel phase-locked loop (PLL) architecture with multiple charge pumps, which is used to design a fast-locking
PLL and a low-phase-noise PLL, is proposed. The effective capacitance and resistance of the loop filter in terms of voltage
is scaled up/down according to the locking status by controlling the magnitude and direction of the charge pump current. Two
PLLs, one with a fast-locking characteristic and the other with a low-phase-noise characteristic, are designed and fabricated
in a 0.35-μm CMOS process based on the proposed architecture. The fast-locking PLL has a locking time of less than 6 μs and
a phase noise of −90.45 dBc/Hz at 1 MHz offset. The low-phase-noise PLL has a locking time of 25 μs, a phase noise of −105.37 dBc/Hz
at 1 MHz offset, and a reference spur of −50 dBc. Both PLLs have an 851.2 MHz output frequency. 相似文献