Prognostic evaluation of early indicators in fulminant hepatic failure by multivariate analysis

Results of eight multicenter, randomized, placebo-controlled, double-blind, parallel-group studies were pooled to assess the efficacy of the angiotensin II-receptor blocker irbesartan over the dose range of 1 to 900 mg. A total of 2955 adults with a seated diastolic blood pressure of 95 to 110 mm Hg were randomized to treatment with oral irbesartan once daily or placebo for 6 to 8 weeks. Office blood pressure was measured at trough (24+/-3 hours after the last dose) and peak (3+/-1 hours after the last dose) by mercury sphygmomanometry. Demographic characteristics (mean blood pressure; 151/101 mm Hg; mean age, 54 years; 63% male; and 82% white) were similar across all dose groups. After the groups were pooled, antihypertensive efficacy was assessed by therapeutic response (trough seated diastolic blood pressure <90 mm Hg or a reduction from baseline of > or = 10 mm Hg) and by modeling of the maximum reductions in trough and peak seated diastolic and systolic blood pressure. Antihypertensive effects increased with increasing doses and reached a plateau at > or = 300 mg. Irbesartan 150 mg provided placebo-subtracted reductions in trough seated systolic and diastolic blood pressure of approximately 8 and approximately 5 mm Hg, respectively, with 56% of patients displaying a favorable response. In conclusion, irbesartan provides clinically significant blood pressure lowering, with a clear relationship between (log) dose and antihypertensive effect.     

An atomic model of the outer layer of the bluetongue virus core derived from X-ray crystallography and electron cryomicroscopy

BACKGROUND: Bluetongue virus (BTV), which belongs to the Reoviridae family and orbivirus genus, is a non-enveloped, icosahedral, double-stranded RNA virus. Several protein layers enclose its genome; upon cell entry the outer layer is stripped away leaving a core, the surface of which is composed of VP7. The structure of the trimeric VP7 molecule has previously been determined using X-ray crystallography. The articulated VP7 subunit consists of two domains, one which is largely alpha-helical and the other, smaller domain, is a beta barrel with jelly-roll topology. The relative orientations of these two domains vary in different crystal forms. The structure of VP7 and the organizations of 780 subunits of this molecule in the core of virus is central to the assembly and function of BTV. RESULTS: A 23 A resolution map of the core, determined using electron cryomicroscopy (cryoEM) data, reveals that the 260 trimers of VP7 are organized on a rather precise T = 13 laevo icosahedral lattice, in accordance with the theory of quasi-equivalence. The VP7 layer occupies a shell that is between 260 A and 345 A from the centre of the core. Below this radius (230-260 A) lies the T = 1 layer of 120 molecules of VP3. By fitting the X-ray structure of an individual VP7 trimer onto the cryoEM BTV core structure, we have generated an atomic model of the VP7 layer of BTV. This demonstrates that one of the molecular structures seen in crystals of the isolated VP7 corresponds to the in vivo conformation of the molecule in the core. CONCLUSIONS: The beta-barrel domains of VP7 are external to the core and interact with protein in the outer layer of the mature virion. The lower, alpha-helical domains of VP7 interact with VP3 molecules which form the inner layer of the BTV core. Adjacent VP7 trimer-trimer interactions in the T = 13 layer are mediated principally through well-defined regions in the broader lower domains, to form a structure that conforms well with that expected from the theory of quasi-equivalence with no significant conformational changes within the individual trimers. The VP3 layer determines the particle size and forms a rather smooth surface upon which the two-dimensional lattice of VP7 trimers is laid down.     

Head injury mechanisms and the concept of preventive management: a review and critical synthesis

Recent advances in head injury research have produced a plethora of useful data coupled with a paucity of conceptual integration across the four ways in which this research is pursued. These research orientations are the epidemiological, biomechanical, basic neuroscientific, and clinicopathologic/therapeutic (including rehabilitation). This overview of the history and current state of the art assumes that biomechanics is the basic science of causation in head injury research and when fully integrated with its counterparts, physiology and pathology, it can serve to overcome our conceptual handicaps. A paradigm integrating biomechanics; into the sequence of preventive, protective, acute therapeutic, and rehabilitative interventions will be described as the concept of preventive management. From this we derive the hypothesized claim that the exact biomechanics and the physiopathologic response at the time of injury (at the macroscopic and microscopic levels) determine the sequence of so-called secondary effects that are conceived as the inexorable delayed manifestations of the primary events and concomitant boundary conditions. Knowledge of these events will enable accurate predictions of the natural history and outcome of head injuries from observations carried out in the early acute phase. Examples to test this claim will be given with particular reference to the two types of traumatic brain injury (TBI) phenomenologically associated with disturbances of consciousness, the onset of which can be either immediate or delayed. The current economics and availability of computational power provide a significant opportunity for the development of selected experimental, physical, and simulated models of head injury on the basis of which the complex neurovascular and nonneural cellular and fluid elements of the nervous system may be accurately modeled. This approach will significantly improve the efficiency and quality of the essential biological and clinical observations and model experiments required to validate the theoretical methods and their predictions.     

Chemical composition and bond structure of carbon-nitride films deposited by CH4/N2 dielectric barrier discharge

Carbon nitride films have been deposited by dielectric barrier discharge with a CH₄/N₂ gas mixture at different conditions. Fourier Transform Infrared (FTIR) spectroscopy, X-ray photo electron spectroscopy (XPS), Raman spectroscopy, Atomic force microscopy (AFM) and ellipsometry were used to systematically study chemical composition, bond structure and surface morphology of deposited films. Various bonds between carbon, nitrogen, hydrogen, and also oxygen were observed.     

Photoelastic Studies on Rubber-to-Rubber Joints

Photoelasticity is a method which yields information on the principal stress difference and orientation in a composite structure. Various problems associated with this technique, especially those concerning the fundamental relationship between the fringe order and the stress, have yet to be investigated. A few studies of this relationship in a universal stress state have been presented, particularly in the field of rubber-to-metal and rubber-to-fabric composites, but no evaluations have so far been made in the field of rubber-to-rubber joints. Applying the photoelastic method, we report our observations on the stress distribution in a natural rubber to natural rubber joint subjected to uniaxial tension. A comparison between the results of experimental photoelastic studies and the corresponding computer modelling has been illustrated. The theoretical displacement pattern of the angular joints of bi-rubber part has also been highlighted.     

Wetting properties of homopolymers and copolymers of pentafluorostyrene and methylacrylate and homopolymer blends

Polypentafluorostyrene (PPFS), polymethylacrylate (PMA), and poly(pentafluorostyrene-co-methylacrylate), poly(PFS-co-MA) were prepared and the wetting characteristics of polymer blends of PPFS and PMA were compared with that of poly(PFS-co-MA) via contact angle measurements. The critical surface tension of polypentafluorostyrene was found to be 22.6 dyne/cm, which is comparable to the value reported for polytrifluoroethylene (22 dyne/cm). The critical surface tension of poly(PFS-co-MA) is not linearly related to its composition. The polymer blends of PPFS and PMA exhibit significant surface enrichment of the fluoropolymer. The harmonic-mean method¹ was employed to determine surface tensions of these polymers and many known polymers. It is found that the method produces useful surface tension data provided the contact angle values are derived from testing liquids of dissimilar polarity.     

Two-stage database intrusion detection by combining multiple evidence and belief update

Insider threats have gained prominence and pose the most challenging threats to a database system. In this paper, we have proposed a new approach for detecting intrusive attacks in databases by fusion of information sources and use of belief update. In database intrusion detection, only intra-transactional features are not sufficient for detecting attackers within the organization as they are potentially familiar with the day-to-day work. Thus, the proposed system uses inter-transactional as well as intra-transactional features for intrusion detection. Moreover, we have also considered three different sensitivity levels of table attributes for keeping track of the malicious modification of the highly sensitive attributes more carefully. We have analyzed the performance of the proposed database intrusion detection system using stochastic models. Our system performs significantly better compared to two intrusion detection systems recently proposed in the literature.     

BLAST-SSAHA Hybridization for Credit Card Fraud Detection

A phenomenal growth in the number of credit card transactions, especially for online purchases, has recently led to a substantial rise in fraudulent activities. Implementation of efficient fraud detection systems has thus become imperative for all credit card issuing banks to minimize their losses. In real life, fraudulent transactions are interspersed with genuine transactions and simple pattern matching is not often sufficient to detect them accurately. Thus, there is a need for combining both anomaly detection as well as misuse detection techniques. In this paper, we propose to use two-stage sequence alignment in which a profile analyzer (PA) first determines the similarity of an incoming sequence of transactions on a given credit card with the genuine cardholder's past spending sequences. The unusual transactions traced by the profile analyzer are next passed on to a deviation analyzer (DA) for possible alignment with past fraudulent behavior. The final decision about the nature of a transaction is taken on the basis of the observations by these two analyzers. In order to achieve online response time for both PA and DA, we suggest a new approach for combining two sequence alignment algorithms BLAST and SSAHA.