With the development of Computer-aided Diagnosis (CAD) and image scanning techniques, Whole-slide Image (WSI) scanners are widely used in the field of pathological diagnosis. Therefore, WSI analysis has become the key to modern digital histopathology. Since 2004, WSI has been used widely in CAD. Since machine vision methods are usually based on semi-automatic or fully automatic computer algorithms, they are highly efficient and labor-saving. The combination of WSI and CAD technologies for segmentation, classification, and detection helps histopathologists to obtain more stable and quantitative results with minimum labor costs and improved diagnosis objectivity. This paper reviews the methods of WSI analysis based on machine learning. Firstly, the development status of WSI and CAD methods are introduced. Secondly, we discuss publicly available WSI datasets and evaluation metrics for segmentation, classification, and detection tasks. Then, the latest development of machine learning techniques in WSI segmentation, classification, and detection are reviewed. Finally, the existing methods are studied, and the application prospects of the methods in this field are forecasted.
Mobile Networks and Applications - The application of the traditional single frame character image super-resolution reconstruction method has some problems, such as noise can not be removed... 相似文献
Two newly discovered bacteriophages, isolated from chicken feces and infecting Salmonella enterica strains, are described in this report. These phages have been named vB_Sen-TO17 and vB_Sen-E22, and we present their molecular and functional characterization. Both studied viruses are able to infect several S. enterica strains and develop lytically, but their specific host ranges differ significantly. Electron microscopic analyses of virions have been performed, and full genome sequences were determined and characterized, along with molecular phylogenetic studies. Genomes of vB_Sen-TO17 (ds DNA of 41,658 bp) and vB_Sen-E22 (dsDNA of 108,987 bp) are devoid of homologs of any known or putative gene coding for toxins or any other proteins potentially deleterious for eukaryotic cells. Both phages adsorbed efficiently (>95% adsorbed virions) within 10 min at 42 °C (resembling chicken body temperature) on cells of most tested host strains. Kinetics of lytic development of vB_Sen-TO17 and vB_Sen-E22, determined in one-step growth experiments, indicated that development is complete within 30–40 min at 42 °C, whereas burst sizes vary from 9 to 79 progeny phages per cell for vB_Sen-TO17 and from 18 to 64 for vB_Sen-E22, depending on the host strain. Virions of both phages were relatively stable (from several percent to almost 100% survivability) under various conditions, including acidic and alkaline pH values (from 3 to 12), temperatures from −80 °C to 60 °C, 70% ethanol, chloroform, and 10% DMSO. These characteristics of vB_Sen-TO17 and vB_Sen-E22 indicate that these phages might be considered in further studies on phage therapy, particularly in attempts to eliminate S. enterica from chicken intestine. 相似文献
Microorganisms such as bacteria and fungi play essential roles in many application fields, like biotechnique, medical technique and industrial domain. Microorganism counting techniques are crucial in microorganism analysis, helping biologists and related researchers quantitatively analyze the microorganisms and calculate their characteristics, such as biomass concentration and biological activity. However, traditional microorganism manual counting methods, such as plate counting method, hemocytometry and turbidimetry, are time-consuming, subjective and need complex operations, which are difficult to be applied in large-scale applications. In order to improve this situation, image analysis is applied for microorganism counting since the 1980s, which consists of digital image processing, image segmentation, image classification and suchlike. Image analysis-based microorganism counting methods are efficient comparing with traditional plate counting methods. In this article, we have studied the development of microorganism counting methods using digital image analysis. Firstly, the microorganisms are grouped as bacteria and other microorganisms. Then, the related articles are summarized based on image segmentation methods. Each part of the article is reviewed by methodologies. Moreover, commonly used image processing methods for microorganism counting are summarized and analyzed to find common technological points. More than 144 papers are outlined in this article. In conclusion, this paper provides new ideas for the future development trend of microorganism counting, and provides systematic suggestions for implementing integrated microorganism counting systems in the future. Researchers in other fields can refer to the techniques analyzed in this paper.
Pattern Analysis and Applications - Training deep neural networks (DNNs) on high-dimensional data with no spatial structure poses a major computational problem. It implies a network architecture... 相似文献
Neural Computing and Applications - The paper is devoted to the problem of a neural network-based robust simultaneous actuator and sensor faults estimator design for the purpose of the fault... 相似文献
Interleukin 18 (IL-18) is a proinflammatory and proatherogenic cytokine with pleiotropic properties, which is involved in T and NK cell maturation and the synthesis of other inflammatory cytokines and cell adhesion molecules. It plays a significant role in orchestrating the cytokine cascade, accelerates atherosclerosis and influences plaque vulnerability. To investigate the influence of IL-18 cytokine on atherosclerosis development, a stochastic Petri net model was built and then analyzed. First, MCT-sets and t-clusters were generated, then knockout and simulation-based analysis was conducted. The application of systems approach that was used in this research enabled an in-depth analysis of the studied phenomenon. Our results gave us better insight into the studied phenomenon and allow revealing that activation of macrophages by the classical pathway and IL-18-MyD88 signaling axis is crucial for the modeled process. 相似文献
Solid‐state refill friction stir spot welding (RFSSW) technology offers significant benefits in the fabrication of aluminium structures in the transport and aerospace industries. In this paper, the joining of 1.6‐mm‐thick Alclad 7075‐T6 aluminium alloy sheets is investigated. High‐cycle fatigue strength tests of single‐lap welded joints were carried out on an Instron E10000 testing machine with a limited number of cycles equal to 2 × 106. The welding of overlap fatigue specimens was conducted using an RPS100 spot welder by Harms & Wende GmbH & Co KG. C‐mode scanning acoustic microscopy (C‐SAM) and scanning electron microscopy (SEM) were utilised to evaluate the joint quality and characterise the microstructure. The paper discusses the effect of the maximum load force and defects (voids, hook, kissing bond, bonding ligament, etc) associated with the material flow in the weld on the failure mechanism. Insufficient plasticisation of sheet material and mixing of the material in the weld area are crucial defects that influence the number of destructive cycles. The weld defects in the joint structure are a source of a decrease in the fatigue life compared with the fatigue life of defect‐free welds. It was also found that RFSSW joint defects can be effectively detected by the nondestructive C‐SAM method. 相似文献
This work concerns an application of the Tsallis entropy to homogenization problem of the fiber‐reinforced and also of the particle‐filled composites with random material and geometrical characteristics. Calculation of the effective material parameters is done with two alternative homogenization methods—the first is based upon the deformation energy of the Representative Volume Element (RVE) subjected to the few specific deformations, while the second uses explicitly the so‐called homogenization functions determined under periodic boundary conditions imposed on this RVE. Probabilistic homogenization is made with the use of three concurrent non‐deterministic methods, namely Monte‐Carlo simulation, iterative generalized stochastic perturbation technique as well as the semi‐analytical approach. The last two approaches are based on the Least Squares Method with polynomial basis of the statistically optimized order— this basis serves for further differentiation in the 10th‐order stochastic perturbation technique, while semi‐analytical method uses it in probabilistic integrals. These three approaches are implemented all as the extensions of the traditional Finite Element Method (FEM) with contrastively different mesh sizes, and they serve in computations of Tsallis entropies of the homogenized tensor components as the functions of input coefficient of variation. 相似文献
The aim of the presented work was to design, formulate and evaluate the properties of low-acyl gellan macro beads with the potential application as carriers for oral delivery of meloxicam (MLX) in the prophylaxis of colorectal cancer. The beads were obtained by means of ionotropic gelation technique. Calcium chloride (1.0%, 9.0?×?10?2 M) was used as the cross-linking agent. Nine different polymer, drug and surfactant (Tween®80) mixtures were used for production of the beads. The quantitative compositions of the mixtures were generated with the application of the Design of Experiments (DoE) modulus from the STATISTICA Software. The prepared formulations revealed 7.2–27.0% of drug loading and 29.2–50.7% drug encapsulation efficiency. It turned out that 0.5% amount of gellan gum in the mixtures was not sufficient to obtain spherical beads. The morphology and surface of the dried beads were analyzed by SEM. Raman spectra confirmed that MLX did not undergo structural changes during production of the beads. The swelling behavior and degradation of the beads were evaluated in three simulated gastrointestinal fluids at different pH (1.2; 4.5; 6.8). The MLX in vitro release studies were conducted on USP apparatus IV, working in the open loop mode. The obtained results showed that MLX release from the dried beads was pH-dependent. The formulations obtained from mixtures containing 1.0 and 1.5% of gellan may be considered as oral dosage forms for MLX, intended to omit the stomach and release the drug in the distal parts of the gastrointestinal tract. 相似文献