Immunoelectron localization of mitochondrial F1 factor in cryosections of heart muscle cells

Immunocytochemistry was used to investigate the localization of F1 ATPase in mitochondria of cryosections of adult mouse heart muscle cells. The initial aldehyde fixation was the only denaturation step for antigens. The fine structure was preserved with contrast enhancement as the sections were maintained hydrated, with the advantage that the entire procedure is completed in one working day. The reaction was highly specific, and entire mitochondria were labeled with the Protein A-gold complex. A new analytical technique, electron spectroscopic imaging (ESI), contributed to a better visualization of the localization of the F1 factor.     

The architecture of the Sure System 2000 communications processor

Sure System 2000, a fault-tolerant computer that couples multiprocessors to offer low-priced, high-performance systems that deal effectively with faults and failures, is presented. The architecture is based on the local redundancy technique, ensuring that no hardware or software fault can cause a system crash. Software errors can be fixed, and hardware can be replaced, upgraded, or added dynamically. Existing fault-tolerant computers are briefly reviewed, and the logic hardware system configuration of the Sure System 2000 is described. The multiprocessor and I/O architecture are examined. The SXO Sure System 2000 expandable operating system is described     

Structural Response of Nuclear Containment Shield Buildings with Construction Openings

Steam generator replacement at nuclear power plants requires large construction openings in nuclear containment structures. This study examines the effects of such openings placed in a 61?cm (2?ft) thick dome of a shield building. The dome is cast in two layers, and the limiting cases of the two layers acting as one and the lower layer carrying the entire load are considered. Without openings, the stresses due to dead load in the structure are very low when compared to the material strength. With openings, the loads are easily redistributed around the opening, and the maximum stresses for the conservative case are between three and four and one-half times larger than in the original structure with no openings. The extent of the affected area around the holes is relatively small. Smooth corners in the openings are necessary to avoid high local stress concentrations.     

Pervasive 2007: It's about the User

Pervasive computing is as much about the user as it is about the technology. So, the Fifth International Conference on Pervasive Computing emphasized desirability rather than just feasibility. Featured themes included user benefits, human-computer interaction, group and social interactions, context awareness, finding and positioning people and objects, and personal privacy.     

Development of New Sulphonyl Resin from Modification of Commercial Resin

Summary This article deals with the handy synthesis of sulfonyl resins, which were produced by the treatment of a commercial sulfonic resin (Lewatit VPOC1812^® based at divinylbenzene (DVB) and styrene (STY). The preliminary chemical modification was based on the reaction of the Lewatit VPOC1812^® with thionyl chloride aiming to produce the sulfonyl chloride groups. The best conditions to obtain the sulfonyl chloride groups were: SOCl₂/SO₃H (molar ratio) =13 at 79 °C during 72 h. The resin chlorinated was afterward treated with urea, thiourea or guanidine. The functionalized resins with urea, thiourea or guanidine were produced with 56, 68 and 93% yield, respectively. The commercial and modified resins were characterized by apparent density, swelling degree, elemental analysis (CHNS), FTIR, optical microscopy (OM) and scanning electron microscopy (SEM).     

Aging time dependence of catastrophic optical damage (COD) failureof a 0.98-μm GaInAs-GaInP strained quantum-well laser

In this paper, we studied the aging time dependence of the catastrophic optical damage (COD) failure of an Al-free uncoated 0.98-μm GaInAs-GaInP strained quantum-well laser with an injection current as a parameter. Based on the stress-strength model, we first investigated experimentally the dependence of the critical power level (CPL) at which COD would take place upon the aging time. Then applying a statistical treatment to this result, we found for the first time that CPL data at each aging time could be considered to distribute according to the Weibull statistics, and the decrease rate of the CPL with the aging time depended very strongly on the injection current. Finally, using the relationship between the decrease rate of the CPL with the aging time and the current, we predicted roughly the time of a COD failure occurrence for both large and small current cases. As a result, we clarified that for our Al-free uncoated 0.98-μm laser, a COD failure became a fatal problem in the case of a large-current (high-power) operation     

On the Limitation of Net Algorithm for Enumerating Isomeric Structures of Giant Fullerenes

Application of our net algorithm to the generation of all possible IPR isomers for giant fullerenes C_n, n=102 to 120, missed nine out of 39,621 possible structures. Analysis revealed that the omission of too small and too large cap triangles was the reason. Within the range of fullerenes studied, the missed structures are of high-energy and do not affect the distribution of significantly low-energy isomers.     

Inhibitory effect of bifemelane on superoxide generation by activated neutrophils measured using a simple chemiluminescence method

We evaluated the effect of 4-(2-benzylphenoxy)-N-methylbutylamine hydrochloride (bifemelane hydrochloride) on superoxide production by human neutrophils using an MCLA-dependent chemiluminescence assay. Bifemelane hydrochloride dose-dependently inhibited superoxide production by neutrophils stimulated with phorbol myristate acetate, opsonized zymosan, or N-formyl-methionyl-leucyl-phenylalanine, while it had no effect on superoxide production by a hypoxanthine-xanthine oxidase system. These results indicate that bifemelane hydrochloride does not have a scavenging effect, but has an inhibitory effect on superoxide generation by neutrophils. Although this drug is commonly used for treating chronic cerebral infarction, it may also have a protective effect on acute ischemia/reperfusion injury.