Melting behavior of polypropylene fibers studied by differential scanning calorimetry

Polypropylene fibers produced in a compact-spinning process were studied by differential scanning calorimetry (DSC). With unrestrained fibers, the onset of melting increases with decreasing draw ratio, increasing M_w/M_n, decreasing extrusion temperature, increasing annealing ratio, and increasing draw-down ratio. These trends are discussed in terms of restraints and reorganization. The onset of melting is shifted to lower temperatures as the heating rate increases for all combinations of material and processing parameters, indicating suppressed reorganization. At low draw ratios, the height and width of the endotherm are affected by the spinline stress, and a secondary peak or shoulder is observed on the high temperature side of the main peak. The magnitude of the secondary peak increases with decreasing M_w/M_n, increasing draw ratio, decreasing draw-down ratio, and decreasing heating rate, but its position mainly depends on the heating rate. This indicates that the secondary peak may be due to the melting of structures that have been reorganized during the heating scan. As the draw ratio increases, the melting regime broadens, especially towards lower temperatures, and several maxima emerge on the DSC curve. Reorganization and shrinkage during heating may explain these observations. © 1995 John Wiley & Sons, Inc.     

Surface Tension Measurements on CMSX-4 Superalloy by the Drop-Weight and Oscillating-Drop Methods

The surface tension of the CMSX-4^®reg; superalloy has been determined by the drop-weight and oscillating-drop methods which are well adapted to reactive materials. The recommended values are 1.59 J·m^–2 for the surface tension at the liquidus temperature and –0.14× 10^–3 J·m^–2· K^–1 for the temperature coefficient. A conclusion of the present work is that the interpretation of surface tension measurements performed on a complex alloy generally requires additional work to be performed on simpler associated binary or ternary systems, as well as some support from solidification experiments.     

Connectivity, clusters, and transport: use of percolation concepts and atomistic simulation to track intracellular ion migration

The cytoskeleton is an intracellular highway system, teaming with signalling ions that zip from site to site along filaments. These tiny particles alternately embrace and slip free of protein receptors with wide-ranging affinities, as they propagate in a blur of motion along cytoskeletal corridors at transport rates far exceeding ordinary diffusive motion. Recent experimental breakthroughs have enabled optical tracking of these single ion-binding events in the physiological and diseased states. However, traditional continuum modelling methods have proven ineffective for modelling migration of biometals such as copper and zinc, whose cytosolic concentrations are putatively vanishingly small, or very tightly controlled. Rather, the key modelling problem that must be solved for biometals is determination of the optimal placement of biosensors that bind and detect the metal ions within the heterogeneous environment of the cell. We discuss herein how percolation concepts, in combination with atomistic simulation and sensor delivery models, have been used to gain insights on this problem, and a roadmap for future breakthroughs.     

Learning eigenfunctions links spectral embedding and kernel PCA

In this letter, we show a direct relation between spectral embedding methods and kernel principal components analysis and how both are special cases of a more general learning problem: learning the principal eigenfunctions of an operator defined from a kernel and the unknown data-generating density. Whereas spectral embedding methods provided only coordinates for the training points, the analysis justifies a simple extension to out-of-sample examples (the Nystr?m formula) for multidimensional scaling (MDS), spectral clustering, Laplacian eigenmaps, locally linear embedding (LLE), and Isomap. The analysis provides, for all such spectral embedding methods, the definition of a loss function, whose empirical average is minimized by the traditional algorithms. The asymptotic expected value of that loss defines a generalization performance and clarifies what these algorithms are trying to learn. Experiments with LLE, Isomap, spectral clustering, and MDS show that this out-of-sample embedding formula generalizes well, with a level of error comparable to the effect of small perturbations of the training set on the embedding.     

Protease-induced aggregation of bovine alpha-lactalbumin: identification of the primary associating fragment

Details in the hydrolysis of alpha-lactalbumin known to result in formation of highly ordered nanotubules, was investigated by incubation of solutions with 10 g alpha-lactalbumin/l with a specific protease from Bacillus licheniformis (BLP). After 50 min of incubation, soluble aggregates were formed, the concentration of which increased until precipitation occurred after 200 min. The latter aggregates were dissolved in urea or at low pH, like the nanotubules characteristic of gels formed by the action of BLP on alpha-lactalbumin at 100 g/l. On the molecular level, alpha-lactalbumin was initially cleaved into two large hydrophobic fragments with masses of 11.6 and 11.3 kDa, which in turn were cleaved in a stepwise manner into the ultimate fragment of 8.8 kDa. This fragment was the predominating component in the insoluble aggregates, and was identified as the sequences 26-37 and 50-113 of alpha-lactalbumin linked together by a disulphide bond. Cleavage of alpha-lactalbumin into this fragment probably created new hydrophobic surfaces and new calcium binding sites allowing its association into ordered structures.     

Detection of phosphorylated peptides in proteomic analyses using microfluidic compact disk technology

A compact disk (CD)-based microfluidic method for selective detection of phosphopeptides by mass spectrometry is described. It combines immobilized metal affinity chromatography (IMAC) and enzymatic dephosphorylation. Phosphoproteins are digested with trypsin and processed on the CD using nanoliter scale IMAC with and without subsequent in situ alkaline phosphatase treatment. This is followed by on-CD matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. Dephosphorylation of the IMAC-enriched peptides allows selective phosphopeptide detection based on the differential mass maps generated (mass shifts of 80 Da or multiples of 80 Da). The CD contains 96 microstructures, each with a 16 nL IMAC microfluidic column. Movement of liquid is controlled by differential spinning of the disk. Up to 48 samples are distributed onto the CD in two equal sets. One set is for phosphopeptide enrichment only, the other for identical phosphopeptide enrichment but combined with in situ dephosphorylation. Peptides are eluted from the columns directly into MALDI target areas, still on the CD, using a solvent containing the MALDI matrix. After crystallization, the CD is inserted into a MALDI mass spectrometer for analysis down to the femtomole level. The average success rate in phosphopeptide detection is over 90%. Applied to noncharacterized samples, the method identified two novel phosphorylation sites, Thr 735 and Ser 737, in the ligand-binding domain of the human mineralocorticoid receptor.     

Moral judgment and the development of real and ideal androgynous self-image during adolescence and young adulthood.

116 Ss at 13, 17, and 20 yrs of age responded to the Defining Issues Test and to the Bem Sex-Role Inventory (BSRI). The BSRI was divided into real self-image and ideal self-image formats. 17-yr-old and college-age males showed less preference on ideal image for masculine characteristics and more preference for feminine characteristics compared to 13-yr-old males. College-age females had a greater preference for masculine compared to feminine characteristics, whereas younger females had no preference for sex-typed characteristics. Ss at higher stages of moral judgment or with a higher percent of postconventional moral judgment were more likely to incorporate aspects of the opposite sex in their self-image. The findings are discussed in terms of both L. Kohlberg's (1966) cognitive developmental theory of sex role development and J. Loevinger's (1976) theory of ego development. (25 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Application of avidin-biotin technology and adsorptive transfer stripping square-wave voltammetry for detection of DNA hybridization and avidin in transgenic avidin maize

The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.     

Development of LC/MS/MS methods for cocktail dosed Caco-2 samples using atmospheric pressure photoionization and electrospray ionization

Good reliability of Caco-2 permeability studies requires competent sampling and analytical methods to ensure the comparability of day-to-day experiments. In this work, two n-in-one LC/MS/MS methods based on two different ionization techniques were developed and validated for a group of reference compounds; eight of them are recommended by the Food and Drug Administration (FDA) for the evaluation of oral drug permeability. The performance of a new ionization technique, atmospheric pressure photoionization (APPI), as an interface for quantitative LC/MS analysis was evaluated in comparison to the electrospray ionization (ESI). Generally, the validation parameters, including sensitivity, accuracy, and repeatability, were comparable for the APPI and ESI methods. The main difference was that the linear quantitative range of APPI was 3-4 orders of magnitude (r(2) >/= 0.998) whereas in ESI it was typically 2-3 orders of magnitude (r(2) >/= 0.990). By the APPI and ESI methods, the simultaneous analysis of nine highly heterogeneous compounds was achieved within 5.5-7 min, which leads to significant savings in time and cost of the analyses. The successful validation data indicate the usefulness of both the methods for the rapid and sensitive (LOD values typically 相似文献