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81.
Clotilde LM Bernard C Sequera DE Karmali A Fusellier A Carter JM 《Journal of laboratory automation》2012,17(4):309-314
Conventional enzyme-linked immunosorbent assay (ELISA) is a gold standard for screening antibodies and testing for protein or antigen presence. A significant limitation of this assay resides in the fact that only one analyte can be assessed per microplate well. Here, we describe and investigate a new technology consisting of an automated ELISA system in which up to 10 analytes can be measured within one single well, thus improving productivity, accuracy, and repeatability by reducing the amount of human labor required. Another strength of the platform is that a user can load any necessary sets/subsets of beads to perform required assays, with improved flexibility compared to manufactured-loaded arrays for multiplex analysis. We also demonstrate that this system can be used to determine the pathogenicity (i.e., presence of Shiga toxins) and serotype (i.e., Escherichia coli O157) of E. coli isolates. 相似文献
82.
In the first section of the article, we examine some recent criticisms of the connectionist enterprise: first, that connectionist models are fundamentally behaviorist in nature (and, therefore, non-cognitive), and second that connectionist models are fundamentally associationist in nature (and, therefore, cognitively weak). We argue that, for a limited class of connectionist models (feed-forward, pattern-associator models), the first criticism is unavoidable. With respect to the second criticism, we propose that connectionist modelsare fundamentally associationist but that this is appropriate for building models of human cognition. However, we do accept the point that there are cognitive capacities for which any purely associative model cannot provide a satisfactory account. The implication that we draw from is this is not that associationist models and mechanisms should be scrapped, but rather that they should be enhanced.In the next section of the article, we identify a set of connectionist approaches which are characterized by active symbols — recurrent circuits which are the basis of knowledge representation. We claim that such approaches avoid criticisms of behaviorism and are, in principle, capable of supporting full cognition. In the final section of the article, we speculate at some length about what we believe would be the characteristics of a fully realized active symbol system. This includes both potential problems and possible solutions (for example, mechanisms needed to control activity in a complex recurrent network) as well as the promise of such systems (in particular, the emergence of knowledge structures which would constitute genuine internal models). 相似文献
83.
提出了一种面向大视场高精度视觉测量的多红外目标靶点(红外LED)亮度自适应控制方法。以微控制器PIC16F873A和LED驱动芯片MAX6964作为核心器件,设计了可满足大空间范围内靶点亮度控制需求的多目标靶点控制驱动器。为了使LED在近距测量时的调节精度和远近距离测量时的发光亮度都能满足要求,根据MAX6964输出特点设计了PWM信号输出电路,并采用推挽电路驱动LED。实验证明,目标靶点在CCD相机上成像光斑稳定一致。 相似文献
84.
A Gaussian sum filter (GSF) with component extended Kalman filters (EKF) is proposed as an approach to localizing an autonomous vehicle in an urban environment with limited GPS availability. The GSF uses vehicle‐relative vision‐based measurements of known map features coupled with inertial navigation solutions to accomplish localization in the absence of GPS. The vision‐based measurements have multimodal measurement likelihood functions that are well represented as weighted sums of Gaussian densities. The GSF is used because of its ability to represent the posterior distribution of the vehicle pose with better efficiency (fewer terms, less computational complexity) than a corresponding bootstrap particle filter with various numbers of particles because of the interaction with measurement hypothesis tests. The expectation‐maximization algorithm is used off line to determine the representational efficiency of the particle filter in terms of an effective number of Gaussian densities. In comparison, the GSF, which uses an iterative condensation procedure after each iteration of the filter to maintain real‐time capabilities, is shown through a series of in‐depth empirical studies to more accurately maintain a representation of the posterior distribution than the particle filter using 37 min of recorded data from Cornell University's autonomous vehicle driven in an urban environment, including a 32 min GPS blackout. © 2012 Wiley Periodicals, Inc. 相似文献
85.
The dynamic capacity theorem characterizes the reliable communication rates of a quantum channel when combined with the noiseless resources of classical communication, quantum communication, and entanglement. In prior work, we proved the converse part of this theorem by making contact with many previous results in the quantum Shannon theory literature. In this work, we prove the theorem with an ??ab initio?? approach, using only the most basic tools in the quantum information theorist??s toolkit: the Alicki-Fannes?? inequality, the chain rule for quantum mutual information, elementary properties of quantum entropy, and the quantum data processing inequality. The result is a simplified proof of the theorem that should be more accessible to those unfamiliar with the quantum Shannon theory literature. We also demonstrate that the ??quantum dynamic capacity formula?? characterizes the Pareto optimal trade-off surface for the full dynamic capacity region. Additivity of this formula reduces the computation of the trade-off surface to a tractable, textbook problem in Pareto trade-off analysis, and we prove that its additivity holds for the quantum Hadamard channels and the quantum erasure channel. We then determine exact expressions for and plot the dynamic capacity region of the quantum dephasing channel, an example from the Hadamard class, and the quantum erasure channel. 相似文献
86.
A rapidly solidified (Co, Fe)3O4 spinel was formed in a cobalt-iron-phosphate glass matrix by gas atomization of melts of composition 37.5% mol % CoO, 37.5%Fe2O3, 25% P2O5; and 40% CoO, 40% Fe2O3, 20% P2O5, and the material has been characterized using size analysis, X-ray diffraction, Mössbauer spectroscopy, and scanning electron microscopy with energy dispersive X-ray spectroscopy.In the 20 mol % P2O5 composition, atomized powder 50–100m in diameter contained 33 wt % (Co, Fe)3O4. The ferrite crystallized as randomly oriented, faceted dendrites parallel to 100, and the growth was apparently diffusion controlled. Unlike the ferrite, where iron is in both tetrahedral and octahedral coordination, the iron in the glassy matrix was predominantly in distorted octahedral coordination. Overall particle size distributions were broad (15–700m), with 50 wt % smaller than 200m. The maximum in the size distribution shifted from 150 to 80m on increasing the atomizing pressure from 7 to 20 bar (100 to 300 psi). Calculations have shown that the cooling rates obtained with oxide melts vary strongly with droplet size, and less strongly with melt temperature. 相似文献
87.
Summary The desire to extend the applicability of the relational model beyond traditional data-processing applications has stimulated interest in nested or non-first normal form relations in which the attributes of a relation can take on values which are sets or even relations themselves. In this paper, we study the role of null values in the nested relational model using an open world assumption. We extend the traditional theory and study the properties of extended operators for nested relations containing nulls. The no-information, unknown, and non-existent interpretation of nulls are discussed and the meaning of empty set is clarified. Finally, contrary to several previous results, we determine that the traditional axiomatization of functional and multivalued dependencies is valid in the presence of nulls.Currently with the Air Force Institute of Technology, AFIT/ENG, Wright-Patterson AFB, OH 45433, USAResearch partially supported by an IBM Faculty Development A ward and NSF grant DCR-8507224 相似文献
88.
Stainless steel fibres in ABS plastic form a composite with an anisotropic resistivity. Samples are rectangular shapes with uniform thickness. By assuming two principal resistivities and by using van der Pauw's technique, we find
2 =
x
y
. For rectangular samples, field theory determines
y
/
x
and hence fibre direction. Results for three sample geometries agree with the theoretical predictions of the fibre patterns and with X-ray data. Samples formed by a centre-sprue feed are the best for fabricating large, uniform samples, while samples with a large length-to-width ratio have the most uniform metal density and fibre orientation. Resistivity was also measured by the more-common two-probe technique. Results correlate well to van der Pauw data, with 95% confidence. 相似文献
89.
M. Z. Harford Mark Rubinstein A. K. Singh A. E. Edelstein S. A. Wolf 《Journal of Superconductivity》1988,1(4):407-415
We have investigated the temperature dependence of the electrical transport and the thermogravimetric properties, from -200°C to +1000, of the Bi-Sr-Ca-Cu-O high-temperature superconductor. We conclude that this system has a cooperative, simultaneous-melting/oxygen desorption/metal-insulator transition that occurs near 900°C. We speculate on its nature and on its relationship to phenomena found in other high-T
c superconductors. 相似文献
90.
The empowerment of service workers: what, why, how, and when 总被引:17,自引:0,他引:17
In recent years, businesses have rushed to adopt an empowerment approach to service delivery in which employees face customers "free of rulebooks," encouraged to do whatever is necessary to satisfy them. But that approach may not be right for everyone. Bowen and Lawler look at the benefits and costs of empowering employees, the range of management practices that empower employees to varying degrees, and key business characteristics that affect the choice of approaches. Managers need to make sure that there is a good fit between their organizational needs and their approach to frontline employees. 相似文献