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排序方式: 共有853条查询结果,搜索用时 15 毫秒
821.
In determining the three-dimensional (3D) structure of macromolecular assemblies in single particle analysis, a large representative dataset of two-dimensional (2D) average images from huge number of raw images is a key for high resolution. Because alignments prior to averaging are computationally intensive, currently available multireference alignment (MRA) software does not survey every possible alignment. This leads to misaligned images, creating blurred averages and reducing the quality of the final 3D reconstruction. We present a new method, in which multireference alignment is harmonized with classification (multireference multiple alignment: MRMA). This method enables a statistical comparison of multiple alignment peaks, reflecting the similarities between each raw image and a set of reference images. Among the selected alignment candidates for each raw image, misaligned images are statistically excluded, based on the principle that aligned raw images of similar projections have a dense distribution around the correctly aligned coordinates in image space. This newly developed method was examined for accuracy and speed using model image sets with various signal-to-noise ratios, and with electron microscope images of the Transient Receptor Potential C3 and the sodium channel. In every data set, the newly developed method outperformed conventional methods in robustness against noise and in speed, creating 2D average images of higher quality. This statistically harmonized alignment-classification combination should greatly improve the quality of single particle analysis. 相似文献
822.
Nishimura M Kanehira S Sakakura M Shimotsuma Y Miura K Hirao K 《Journal of nanoscience and nanotechnology》2011,11(11):10087-10092
Silicon precipitation inside a glass is an important technique for silicon photonics. We successfully precipitated silicon inside silicate glasses containing an Al metal film using femtosecond laser irradiation. First, the Al-inserted sandwiched glass was fabricated by the direct bonding method. The results of a tensile test indicated that the adhesive strength of the sandwich structure reached approximately 4 MPa. Next, femtosecond laser pulses were focused at the Al/glass interface in the sandwich structure. A transmission electron microscopy photograph at the focus of the laser showed that the Al particles were dispersed into the glass substrate to a depth of approximately 2 microm from the initial Al layer. In addition, Raman spectra indicated that silicon had formed at the interface between the glass and Al film after the laser irradiation. The morphology or the particle size of the precipitated silicon was successfully modified by changing the repetition rate or the pulse energy of the laser. 相似文献
823.
824.
Kenjiro Fukuda Tsuyoshi Sekitani Ute Zschieschang Hagen Klauk Kazunori Kuribara Tomoyuki Yokota Takushi Sugino Kinji Asaka Masaaki Ikeda Hirokazu Kuwabara Tatsuya Yamamoto Kazuo Takimiya Takanori Fukushima Takuzo Aida Makoto Takamiya Takayasu Sakurai Takao Someya 《Advanced functional materials》2011,21(21):4001-4001
825.
Silica (SiO2)‐crosslinked polystyrene (PS) particles possessing photofunctional N,N‐diethyldithiocarbamate (DC) groups on their surface were prepared by the free‐radical emulsion copolymerization of a mixture of SiO2 (diameter Dn = 192 nm), styrene, divinyl benzene, 4‐vinylbenzyl N,N‐diethyldithiocarbamate (VBDC), and 2‐hydroxyethyl methacrylate with a radical initiator under UV irradiation. In this copolymerization, the inimer VBDC had the formation of a hyperbranched structure by a living radical mechanism. These particles had DC groups on their surface. Subsequently, poly(methyl methacrylate) brushes encapsulated SiO2 particles were synthesized by the grafting from a photoinduced atom transfer radical polymerization (ATRP) approach of methyl methacrylate initiated by SiO2‐crosslinked PS particles as a macroinitiator. We constructed the colloidal crystals using these photofunctional particles. Moreover, the SiO2 particle array of colloidal crystals was locked by radical photopolymerization with vinyl monomer as a matrix. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011 相似文献
826.
827.
In spinal fixation devices, the Young's modulus of the metallic implant rod should be not only sufficiently low to prevent stress shielding for the patient but also sufficiently high to suppress springback for the surgeon. This paper proposes a novel function of biomedical titanium alloys—self-adjustment of Young's modulus. Deformation-induced ω phase transformation was introduced into β-type titanium alloys so that the Young's modulus of only the deformed part would increase during operation, while that of the non-deformed part would remain low. The Young's modulus increase by deformation was investigated for a binary Ti-12Cr alloy. This alloy successfully underwent deformation-induced ω phase transformation and exhibited the increase in the Young's modulus by deformation. 相似文献
828.
Takeshi Kobayashi Masaaki Ichiki Toshihiko Noguchi Ryutaro Maeda 《Thin solid films》2008,516(16):5272-5276
The present paper describes a Pt/LNO/PZT/LNO/Pt/Ti/SiO2 multilayers deposited on 4-inch Si wafers. We have evaluated the variation of the deflection of the Si wafers with deposition of each of the thin films. The deposition of the multilayers has resulted in downward deflection (center is higher than edge) of the Si wafers. The multilayers have been also deposited onto SOI wafers and fabricated into piezoelectric micro cantilevers through MEMS bulk micromachining. The micro cantilevers have shown the upward deflection. We have characterized the ferroelectric and piezoelectric properties of the PZT thin films through electrical tests of the micro cantilevers. The dielectric constant, saturation polarization, remanent polarization and coercive field were measured to be 1050, 31.3 μC/cm2, 9.1 μC/cm2 and 21 kV/cm, respectively. The transverse piezoelectric constant, d31, was measured to be − 110 pm/V from the DC response of the micro cantilevers. 相似文献
829.
Takechi M Tatehara S Satomura K Fujisawa K Nagayama M 《Journal of materials science. Materials in medicine》2008,19(8):2949-2952
Melatonin influences the release of growth hormone and cortisol in humans, and it was recently reported that it promoted bone formation. On the other hand, fibroblast growth factor-2 (FGF-2) was reported to facilitate the proliferation of osteoblasts. In the present study, we examined the effect of recombinant human FGF-2 and melatonin on the promotion of osteogenesis around titanium implants. Twenty-four 10-week-old female rats of the Wistar strain received titanium implants in both tibiae. In the experimental groups, 100 mg/kg body weight of melatonin was administered by intraperitoneal injection for 4 weeks after implantation and 10 microg of FGF-2 was locally injected around the implant sites 5 days after implantation. The control groups were administered saline only. In the control group, few newly formed bone could be seen around the implants. It was observed to be in direct contact with the implant surface, but otherwise unmineralized connective tissue was occasionally interposed. In the experimental group, newly formed bone was observed around the titanium implant. In addition, in contrast to the control group, abundant bone trabeculae were seen in the medullary canal region. Bone trabeculae were directly connected to existing cortical bone. These results strongly suggested that melatonin and FGF-2 have the potential to promote osseointegration. 相似文献
830.
Kawashita M Domi S Saito Y Aoki M Ebisawa Y Kokubo T Saito T Takano M Araki N Hiraoka M 《Journal of materials science. Materials in medicine》2008,19(5):1897-1903
Ferrimagnetic materials can be expected to be useful as thermo seeds for hyperthermic treatment of cancer, especially where
the cancer is located in deep parts of body, as they can generate heat by magnetic hysteretic loss when they are placed in
an alternating magnetic field. Recently, it was reported that ferrimagnetic maghemite (γ-Fe2O3) microspheres 20–30 μm in diameter prepared in aqueous solution can show excellent heat generating ability. However, these
microspheres have many cracks on their surfaces. In this study, the preparation conditions for the microspheres was further
optimized in order to obtain crack-free ferrimagnetic microspheres, and the in vitro heat generation of the obtained microspheres
was measured in an agar phantom under an alternating magnetic field. Crack-free γ-Fe2O3 microspheres 20–30 μm in diameter were obtained successfully. Their saturation magnetization and coercive force were 68 emu g−1 and 198 Oe, respectively. Their heat generation under an alternating magnetic field of 300 Oe at 100 kHz was estimated to
be 42 W g−1. The microspheres showed in vitro heat generation when they were dispersed in an agar phantom and placed under an alternating
magnetic field. It is believed that these microspheres may be useful for the in situ hyperthermic treatment of cancer. 相似文献