A superhydrophilic titanium implant functionalized by ozone gas modulates bone marrow cell and macrophage responses

Bone-forming cells and M? play key roles in bone tissue repair. In this study, we prepared a superhydrophilic titanium implant functionalized by ozone gas to modulate osteoconductivity and inhibit inflammatory response towards titanium implants. After 24 h of ozone gas treatment, the water contact angle of the titanium surface became zero. XPS analysis revealed that hydroxyl groups were greatly increased, but carbon contaminants were largely decreased 24 h after ozone gas functionalization. Also, ozone gas functionalization did not alter titanium surface topography. Superhydrophilic titanium (O₃–Ti) largely increased the aspect ratio, size and perimeter of cells when compared with untreated titanium (unTi). In addition, O₃–Ti facilitated rat bone marrow derived MSCs differentiation and mineralization evidenced by greater ALP activity and bone-like nodule formation. Interestingly, O₃–Ti did not affect RAW264.7 M? proliferation. However, naive RAW264.7 M? cultured on unTi produced a two-fold larger amount of TNFα than that on O₃–Ti. Furthermore, O₃–Ti greatly mitigated proinflammatory cytokine production, including TNFα and IL-6 from LSP-stimulated RAW264.7 M?. These results demonstrated that a superhydrophilic titanium prepared by simple ozone gas functionalization successfully increased MSCs proliferation and differentiation, and mitigated proinflammatory cytokine production from both naive and LPS-stimulated M?. This superhydrophilic surface would be useful as an endosseous implantable biomaterials and as a biomaterial for implantation into other tissues.     

Isolation and characterization of a novel human pancreas-specific gene, pancpin, that is down-regulated in pancreatic cancer cells

We reviewed 19 children and adolescents with cervical spine congenital synostosis as in Klippel-Feil syndrome (KFS), with an average follow-up of 12.5 years. We paid particular attention to neurologic complications associated with cervical spine abnormalities. Five patients were affected by neurologic complications; four underwent a surgical procedure; and 14 had no neurologic finding. Two had hypermobility at one level, and one had hypermobility at two levels. We found that the more numerous the occipito-C1 abnormalities, the more significant the neurologic risk. In contrast, this risk was not related to the number of "mobile blocks" or to age. Various mechanisms of neural complications have been studied in the literature: medullary abnormality, spinal instability, narrowing of the cervical canal, and vascular dysfunction. Surgery is usually thought to be required in cases with neurologic complications. The indication for surgery is, however, less clear in cases of pure instability without neurologic involvement because surgery is likely to increase the future risks at mobile disks either above or below the fuse level. Careful clinical and radiologic observation is necessary in such patients. Magnetic resonance imaging (MRI) with lateral views in flexion and extension seem to be the best method for detecting impingement of the spine on the cord.     

Relationship of occupation to blood pressure among middle-aged Japanese men--the significance of the differences in body mass index and alcohol consumption

To clarify how and why blood pressure differs between occupations, the proportions of hypertensives, and the measures of blood pressure, body mass index (BMI) and alcohol consumption among the individuals not taking antihypertensive drugs were compared between the eight occupational categories using the data from a health check-up for 589 middle-aged Japanese males, mostly randomly selected from five areas in Japan. After adjusting for age, the relationships of occupation to the proportion of hypertensives and the mean systolic and diastolic blood pressure substantially differed among the five areas. However, after further adjustment for residence, these blood pressure levels (the proportion of hypertensives, and the mean systolic and diastolic blood pressure) were found to be higher for the "Personnel in transport and communications", the "Clerical personnel", the "Managerial and civil personnel" and the "Professional and technical personnel", whereas these values were consistently lowest in the "Service personnel". Age and residence-adjusted mean BMI was also higher for the four occupational categories with the increased blood pressure levels. According to a weighted multiple regression analysis across the eight occupations, the age and residence-adjusted mean BMI was a significant predictor of the age and residence-adjusted mean systolic and diastolic blood pressure (p = 0.068 and 0.018, respectively). These results suggest that the occupation-related changes in BMI may largely contribute to the occupation-related changes in blood pressure.     

Production mechanism of polyzirconocarbo- silane using zirconium(IV)acetylacetonate and its conversion of the polymer into inorganic materials

The reaction of polycarbosilane with zirconium(IV)acetylacetonate proceeded at 573 K in nitrogen atmosphere by the condensation reaction of the Si–H bonds in polycarbosilane and the ligands of zirconium(IV)acetylacetonate accompanied by the evolution of acetylacetone, and then the molecular weight increased by the cross-linking reaction with a formation of Si–Zr bond. The obtained polyzirconocarbosilane showed higher ceramic yield than the polycarbosilane. Zirconium contained in the pyrolysed polyzirconocarbosilane was furthermore found to have the effect of inhibiting crystalline grain growth of -type SiC up to high temperature, so Si–Zr–C–O fibre, which was obtained by the use of polyzirconocarbosilane as precursor, showed high tensile strength up to high temperature.     

Reverse direction-based surrounder queries for mobile recommendations

This paper proposes a new spatial query called a reverse direction-based surrounder (RDBS) query, which retrieves a user who is seeing a point of interest (POI) as one of their direction-based surrounders (DBSs). According to a user, one POI can be dominated by a second POI if the POIs are directionally close and the first POI is farther from the user than the second is. Two POIs are directionally close if their included angle with respect to the user is smaller than an angular threshold ??. If a POI cannot be dominated by another POI, it is a DBS of the user. We also propose an extended query called competitor RDBS query. POIs that share the same RDBSs with another POI are defined as competitors of that POI. We design algorithms to answer the RDBS queries and competitor queries. The experimental results show that the proposed algorithms can answer the queries efficiently.     

Compact electrostatic levitator for diffraction measurements with a two axis diffractometer and a laboratory x-ray source

A compact electrostatic levitator was developed for the structural analysis of high-temperature liquids by x-ray diffraction methods. The size of the levitator was 200 mm in diameter and 200 mm in height and can be set up on a two axis diffractometer with a laboratory x-ray source, which is very convenient in performing structural measurements of high-temperature liquids. In particular, since the laboratory x-ray source allows a great amount of user time, preliminary or challenging experiments can be performed with trial and error, which prepares and complements synchrotron x-ray experiments. The present small apparatus also provides the advantage of portability and facility of setting. To demonstrate the capability of this electrostatic levitator, the static structure factors of alumina and silicon samples in their liquid phases were successfully measured.     

Japanese/English Cross-Language Information Retrieval: Exploration of Query Translation and Transliteration

Cross-language information retrieval (CLIR), where queriesand documents are in different languages, has of late become one ofthe major topics within the information retrieval community. Thispaper proposes a Japanese/English CLIR system, where we combine aquery translation and retrieval modules. We currently target theretrieval of technical documents, and therefore the performance of oursystem is highly dependent on the quality of the translation oftechnical terms. However, the technical term translation is stillproblematic in that technical terms are often compound words, and thusnew terms are progressively created by combining existing basewords. In addition, Japanese often represents loanwords based on itsspecial phonogram. Consequently, existing dictionaries find itdifficult to achieve sufficient coverage. To counter the firstproblem, we produce a Japanese/English dictionary for base words, andtranslate compound words on a word-by-word basis. We also use aprobabilistic method to resolve translation ambiguity. For the secondproblem, we use a transliteration method, which corresponds wordsunlisted in the base word dictionary to their phonetic equivalents inthe target language. We evaluate our system using a test collectionfor CLIR, and show that both the compound word translation andtransliteration methods improve the system performance.     

The influence of carboxylate ions on the growth of β-FeOOH particles

Colloidal -FeOOH particles were produced by hydrolysis of FeCl₃ solutions doped with varied amounts of formate, lactate, oxalate, tartarate, pyromellitate and EDTA ions at 100 °C. The resulting particles were characterized by X-ray diffraction, transmission electron microscopy, thermogravimetric-differential thermal analysis, Fourier transform-infrared spectroscopy, elemental analysis, and the adsorption of nitrogen and water. With increasing concentration of added carboxylate ions, the mean modal size of the particles formed increased at low concentrations and decreased after passing the maximum. The crystallite sizes also revealed a maximum on adding EDTA, oxalate and lactate ions, while they monotonically decreased with the addition of other ions. Doping with tartarate and pyromellitate ions by more than 7 and 10 mol%, respectively, produced amorphous particles containing a large amount of these ions. The particles formed with 20 mol% tartarate ions adsorbed water selectively.     

Contributions of distinct prefrontal neuron classes in reward processing

The prefrontal cortex(PFC)is thought to be involved in higher order cognitive functions,such as in working memory,abstract categorization,and reward processing.It has been reported that two distinct neuron classes(putative pyramidal cells and interneurons)in the PFC played different functional roles in neural circuits involved in forming working memory and abstract categories.However,it remains elusive how the two types of neurons process reward information in the PFC.To investigate this issue,the activity of single neurons was extracellularly recorded in the PFC of the monkey performing a reward predicting task.PFC neurons were classified into putative pyramidal cells and interneurons,respectively,based on the waveforms of action potentials.Both the two types of neurons encoded reward information and discriminated two reward conditions(the preferred reward condition vs.the nonpreferred reward condition).However,the putative pyramidal neurons had better and more reliable discriminability than the putative interneurons.Also,the pyramidal cells represented reward information in the preferred reward condition,but not in the nonpreferred reward condition by raising their firing rates relative to the baseline rates.In contrast,the interneurons encoded reward information in the nonpreferred reward condition,but not in the preferred reward condition by inhibiting their discharge rates relative to the baseline rates.These results suggested that the putative pyramidal cells and interneurons had complementary functions in reward processing.These findings may help to clarify individual functions of each type of neurons in PFC neuronal circuits involved in reward processing.