Increased sensitivity of gastric cancer cells to natural killer and lymphokine-activated killer cells by antisense suppression of N-acetylgalactosaminyltransferase

UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferases (GalNAc transferases) catalyze the initial reaction in O-linked (mucin type) oligosaccharide biosynthesis. To attempt to inhibit the synthesis of O-glycan, we transfected antisense cDNA of GalNAc transferase type 1 (GalNAc-T1) into a human gastric cancer cell line, JRST. A decreased expression of GalNAc-T1 at the level of both mRNA and protein was observed in the resultant transfectants. They demonstrated a significantly increased sensitivity to NK and lymphokine-activated killer cells in vitro compared with parental cells and mock transfectants. Although there was no significant difference in in vitro cell proliferation among parental cells, mock transfectants, or antisense transfectants, the in vivo growth rate of antisense transfectants using SCID mice was clearly lower than that of parental cells and mock transfectants. Furthermore, the treatment of mice with anti-asialo-G(M1) Ab abolished this growth-inhibitory effect of antisense transfection. From these results, we conclude that antisense suppression of GalNAc-T1 could increase the sensitivity of tumor cells to NK and lymphokine-activated killer cells, suggesting that this strategy may be of use as a new immunogene therapy.     

Mmic technology for communication systems

MMIC technology is recently progressing at a rapid rate and is now being applied in communications systems. However, there remain few practical applications. This is mainly due to the high cost of conventional mmics because of the small market size and specialized needs. This paper introduces three new technical approaches that overcome the problems: uniplanar mmic, line unified fet^lufet), and multilayer mmic. Concepts and several examples of these technologies are described. It is shown that these technologies are effective not only for cost reduction but also for increased performance. In addition, one example of system application is described.     

99Tcm-MAG3: a sensitive indicator for evaluating perfusion and rejection of renal transplants

Radionuclide renography has a role in evaluating perfusion of transplanted kidneys. In the course of rejection, cortical perfusion decreases before urinary excretion changes. Based on the facts that 99Tcm-MAG3 has different pharmacokinetics and shows a higher kidney-to-background count ratio than 99Tcm-DTPA, we postulated that 99Tcm-MAG3 was a sensitive and reproducible agent to measure cortical perfusion of transplanted kidneys. To clarify the feasibility of using 99Tcm-MAG3 to measure the cortical perfusion index (CPI), sequential renography was performed using 99Tcm-DTPA and 99Tcm-MAG3 in 14 patients with stable renal transplants, who had changes in serum creatinine concentration of less than 50% between the two studies. The CPI was calculated with 99Tcm-DTPA and 99Tcm-MAG3 and these were then compared and correlated with concurrent serum creatinine concentration. The CPI with 99Tcm-MAG3 was 1.43 times that with 99Tcm-DTPA in patients with changes in serum creatinine concentration equal to or less than 20%, and regression analysis revealed that the difference in CPI was larger in patients with more severely decreased renal perfusion than in patients with normal or mildly decreased renal perfusion. This preliminary study has indicated that the CPI with 99Tcm-MAG3 is a sensitive index for detecting changes in renal function, and thus is a feasible indicator of cortical perfusion when evaluating the rejection of transplanted kidneys.     

Idiopathic restrictive cardiomyopathy with diffuse perimyocytic fibrosis--a rare observation

Idiopathic restrictive cardiomyopathy (RCM) is rare. Even today little is understood of its etiology or underlying mechanisms, and definitive diagnostic criteria are lacking. In this report, we describe a case of idiopathic RCM in a young Japanese woman who died while awaiting cardiac transplantation during the 5-year course of the disease. Rare pathologic findings of diffuse perimyocytic fibrosis were revealed at autopsy.     

Development of extruded ethylene propylene rubber insulated superconducting cable

A superconducting power cable is one of the promising ways of underground transmission of huge electric power in the future. The authors have long proposed the idea of the extruded polymer insulation for superconducting cables. The prominent features of the design are to exploit the excellent electrical properties of polymer in the cryogenic temperatures and to separate the helium coolant from the electrical insulation. Although the extruded cross-linked polyethylene cable has proved ability at the liquid nitrogen temperature, the cable insulation cracked due to mechanical stress during cooling to the liquid helium temperature. To overcome this problem, ethylene propylene rubber (EPR) was selected as a new insulating material considering the good results of mechanical and electrical tests of EPR samples at cryogenic temperatures. An extruded EPR insulated superconducting cable 15 m in length was fabricated and a cooling test down to the liquid helium temperature and a voltage test at the liquid helium temperature were carried out with fair success. This is a breakthrough in terms of the electrical insulation design of cryogenic cables.     

Phorbol ester stimulates PAF synthesisvia the activation of protein kinase C in rat leukocytes

When rat pleural mononuclear leukocytes were stimulated with 1 μM phorbol myristate acetate (PMA), platelet-activating factor (PAF)-like activity was detected in the supernatant and the cellular fractions of the incubation mixture, as measured by rabbit platelet aggregation. C₁₆PAF activity peaked at 30 min in both fractions. Acetyltransferase activity in the microsomal fraction of the stimulated cells also increased rapidly and showed a peak at 10 min. A protein kinase C inhibitor, staurosporine, and an inhibitor of phospholipase A₂,p-bromophenacylbromide, inhibited stimulated PAF formation in both fractions. Staurosporine also inhibited PMA induced acetyltransferase activity. The data suggest that PMA stimulates PAF synthesis by the remodeling pathway in rat pleural cells through activation of both phospholipase A₂ and acetyltransferase, and that the acetyltransferase, in turn, may be activated through activation of protein kinase C. Based on a paper presented at the Third International Conference on Platelet-Activating Factor and Structurally Related Alkyl Ether Lipids, Tokyo, Japan, May 1989.     

A 2-V, 1-10 GHz BiCMOS transceiver chip for multimode wirelesscommunications networks

This paper concerns the design consideration, fabrication process, and performance results for an ultra-broadband, low-voltage, low-power, BiCMOS-based transceiver chip for cellular-satellite-LAN wireless communication networks. The transceiver chip incorporates an RF amplifier, a Gilbert down-mixer, and an IF amplifier in the receive path, and an IF amplifier, a Gilbert up-mixer, and an RF amplifier in the transmit path. For an RF frequency in the 1-10 GHz band and an IF frequency in the 100-1000 MHz band, the developed transceiver chip consumes less than 60 mW at 2 V, to yield a downconversion gain of 40 dB at 1 GHz and 10 dB at 10 GHz and an upconversion gain of 42 dB at 1 GHz and 11 dB at 10 GHz. To avoid possible start-up problems caused during “stand-by” to “enable” mode transition, a simple switching technique is employed for enabling either the receive or the transmit path, by changing the value of a reference voltage applied to both the down- and the up-mixers. While the developed transceiver chip exhibits the best performance for a dc supply voltage of 2 V, it shows a graceful degradation for a ±0.15 V voltage deviation. The transceiver's chip size is 1.04 mm×1.04 mm     

Development of a compact high-density microbial hydrogen reactor for portable bio-fuel cell system

The development of a compact high-density microbial reactor for hydrogen production is described with possible implications to use as a portable bio-fuel cell system. To construct the compact bioreactor, mainly, the cell density and immobilization methods were optimized in this paper. The encapsulation of hydrogen producing bacterium, Escherichia coli strain MC13-4, in alginate gel beads provided approximately three-fold increase in hydrogen production in comparison with the free cell suspension. The immobilized cells (cell density; O.D. 100) and 500 mM glucose solution were packed into a 20 mL glass bottle that was connected to the fuel cell. This system has generated electricity of over 20 mW for 20 min.     

Multilateral analysis of increasing collective dose and new ALARA programme

JAPC (The Japan Atomic Power Company) is the only electric power company that operates different types of nuclear reactors in Japan; it operates two BWRs (boiling water reactors), one pressurised water reactor and one gas cooled reactor. JAPC has been conducting various activities aimed at reducing radiation dose received by workers for over 45 y. Recently, the collective dose resulting from periodic maintenance has increased at each plant because of the replacement of large equipment and the unexpected extension of the outage period. In particular, the collective dose at Tokai-2 is one of the highest among Japanese BWR plants((1)), owing to the replacement and strengthening of equipment to meet earthquake-proof requirements. In this study, the authors performed a multilateral analysis of unacceptably a large collective dose and devised a new ALARA programme that includes a 3D dose prediction map and the development of machines to assist workers.     

A Hydrogen Management Method in Residential Distributed Generation Systems with Photovoltaic Cells and Hydrogen‐Storage Type Fuel Cells

Recently, renewable energy has been attracting attention as a result of global warming and the depletion of fossil fuels. Photovoltaic (PV) systems have spread rapidly around the world because they generate electric power quietly and can be installed in many places. The output power generated in a PV system fluctuates with changes in solar irradiance and panel temperature. The reverse flow of surplus power in output spikes may have a negative effect on electric power quality, such as on the frequency and voltage in a power system. A residential distributed generation (DG) system composed of a fuel cell (FC) unit, an electrolyzer (EL) unit, and a PV system has been proposed in order to resolve these problems. In order to operate this system without interruption, the hydrogen storage volume must be managed. This paper proposes a novel hydrogen management method for a residential DG system with PV cells and hydrogen‐storage type FCs. The hydrogen storage volume is maintained at the preset frequency by operating the FC unit and the EL unit. Models of the PV, FC, and EL were constructed for a simulation. In the simulation, we showed that the proposed management method is viable for a residential DG system with PV cells and hydrogen‐storage FCs.