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排序方式: 共有507条查询结果,搜索用时 15 毫秒
71.
Fernández Eduardo Collet Maxime Alarcón Pablo Bauduin Simon Duysinx Pierre 《Structural and Multidisciplinary Optimization》2019,60(5):2113-2130
Structural and Multidisciplinary Optimization - The maximum size constraint restricts the amount of material within a test region in each point of the design domain, leading to a highly constrained... 相似文献
72.
73.
Engineered Ferritin for Magnetogenetic Manipulation of Proteins and Organelles Inside Living Cells 下载免费PDF全文
Domenik Liße Cornelia Monzel Chiara Vicario John Manzi Isabelle Maurin Mathieu Coppey Jacob Piehler Maxime Dahan 《Advanced materials (Deerfield Beach, Fla.)》2017,29(42)
Magnetogenetics is emerging as a novel approach for remote‐controlled manipulation of cellular functions in tissues and organisms with high spatial and temporal resolution. A critical, still challenging issue for these techniques is to conjugate target proteins with magnetic probes that can satisfy multiple colloidal and biofunctional constraints. Here, semisynthetic magnetic nanoparticles are tailored based on human ferritin coupled to monomeric enhanced green fluorescent protein (mEGFP) for magnetic manipulation of proteins inside living cells. This study demonstrates efficient delivery, intracellular stealth properties, and rapid subcellular targeting of those magnetic nanoparticles via GFP–nanobody interactions. By means of magnetic field gradients, rapid spatial reorganization in the cytosol of proteins captured to the nanoparticle surface is achieved. Moreover, exploiting efficient nanoparticle targeting to intracellular membranes, remote‐controlled arrest of mitochondrial dynamics using magnetic fields is demonstrated. The studies establish subcellular control of proteins and organelles with unprecedented spatial and temporal resolution, thus opening new prospects for magnetogenetic applications in fundamental cell biology and nanomedicine. 相似文献
74.
Experimental investigations of non‐Newtonian/Newtonian liquid‐liquid flows in microchannels 下载免费PDF全文
Evangelia Roumpea Maxime Chinaud Panagiota Angeli 《American Institute of Chemical Engineers》2017,63(8):3599-3609
The plug flow of a non‐Newtonian and a Newtonian liquid was experimentally investigated in a quartz microchannel (200‐µm internal diameter). Two aqueous glycerol solutions containing xanthan gum at 1000 and 2000 ppm were the non‐Newtonian fluids and 0.0046 Pa s silicone oil was the Newtonian phase forming the dispersed plugs. Two‐color particle image velocimetry was used to obtain the hydrodynamic characteristics and the velocity profiles in both phases under different fluid flow rates. The experimental results revealed that the increase in xanthan gum concentration produced longer, bullet‐shaped plugs, and increased the thickness of the film surrounding them. From the shear rate and viscosity profiles, it was found that the polymer solution was in the shear‐thinning region while the viscosity was higher in the middle of the channel compared to the region close to the wall. Circulation times in the aqueous phase increased with the concentration of xanthan gum. © 2017 The Authors AIChE Journal published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers AIChE J, 63: 3599–3609, 2017 相似文献
75.
Maxime Guillerm Annabelle Couvert Abdeltif Amrane Edith Norrant Audrey Breton ric Dumont 《中国化学工程学报》2017,25(10):1512-1518
Toluene degradation performances were studied in a 10 L Two-Phase Partitioning Bioreactor (TPPB).The liquid phase consisted of a mixture of water and PDMS 50 (PolyDiMethylSiloxane,i.e.silicone oil,viscosity of 46 maa·s) in the volume ratio of 75%/25%.Two series of experiments were carried out:in the first,the reactor was sequentially supplied with toluene whereas in the second,toluene was continuously supplied.Activated sludge from the wastewater treatment plant of Beaurade (Rennes,France) was used at an initial concentration of 0.5 dry mass g· (mixture L)-1.The elimination capacity (EC) was investigated as well as the change in biomass concentration over time.Toluene biodegradation was very efficient (removal efficiency,RE =100%) for toluene flows ranging from 0.2 to 1.2 ml· h-1,corresponding to elimination capacities of up to 104 g· m-3· h-1.For a toluene flow of 1.2 ml·h-1,the biomass concentration measured at the end of the experiment was 4.7 dry mass g.(mixture L)-1.The oxygen concentration in the liquid phase was clearly not a limiting factor in these operating conditions.Based on these results,an extrapolation leading to the design of a large-scale pilot TPPB can now be considered to study toluene degradation performances in industrial conditions. 相似文献
76.
Franois Ozanam Charlotte Vieillard Marcel Warntjes Thomas Dubois Maxime Pauly Jean-Noël Chazalviel 《加拿大化工杂志》1998,76(6):1020-1026
The hydrogentated surface of silicon exhibits remarkable properties, but poor resistance to oxidation. To improve its stability, surface hydrogen has been replaced by several organic groups. Such grafting can be carried out chemically by a multi-step reaction scheme. However, an electrochemical approach allows direct reaction with the hydrogenated surface. The porous-silicon surface has been partially methoxylated by a controlled anodic dissolution. If has also been methylated using a non-destructive anodic process, with a yield of 80%, limited only by steric hindrance. The methylated surface of porous silicon exhibits a stability against oxidation increased by an order of magnitude. 相似文献
77.
T. Matsumoto M. Kubota S. Matsuoka P. Ginet J. Furtado F. Barbier 《International Journal of Hydrogen Energy》2017,42(11):7422-7428
In order to determine appropriate value for threshold stress intensity factor for hydrogen-assisted cracking (KIH), constant-displacement and rising-load tests were conducted in high-pressure hydrogen gas for JIS-SCM435 low alloy steel (Cr-Mo steel) used as stationary storage buffer of a hydrogen refuelling station with 0.2% proof strength and ultimate tensile strength equal to 772 MPa and 948 MPa respectively. Thresholds for crack arrest under constant displacement and for crack initiation under rising load were identified. The crack arrest threshold under constant displacement was 44.3 MPa m1/2 to 44.5 MPa m1/2 when small-scale yielding and plane-strain criteria were satisfied and the crack initiation threshold under rising load was 33.1 MPa m1/2 to 41.1 MPa m1/2 in 115 MPa hydrogen gas. The crack arrest threshold was roughly equivalent to the crack initiation threshold although the crack initiation threshold showed slightly more conservative values. It was considered that both test methods could be suitable to determine appropriate value for KIH for this material. 相似文献
78.
Moreau-Villéger V Delingette H Sermesant M Ashikaga H McVeigh E Ayache N 《IEEE transactions on bio-medical engineering》2006,53(8):1457-1466
In this paper, we address the problem of estimating the parameters of an electrophysiological model of the heart from a set of electrical recordings. The chosen model is the reaction-diffusion model on the transmembrane potential proposed by Aliev and Panfilov. For this model of the transmembrane, we estimate a local apparent two-dimensional conductivity from a measured depolarization time distribution. First, we perform an initial adjustment including the choice of initial conditions and of a set of global parameters. We then propose a local estimation by minimizing the quadratic error between the depolarization time computed by the model and the measures. As a first step we address the problem on the epicardial surface in the case of an isotropic version of the Aliev and Panfilov model. The minimization is performed using Brent method without computing the derivative of the error. The feasibility of the approach is demonstrated on synthetic electrophysiological measurements. A proof of concept is obtained on real electrophysiological measures of normal and infarcted canine hearts. 相似文献
79.
Pressure difference inside the Polymer Electrolyte Membrane Fuel Cells (PEMFC) arises due to load variations, during which the pressure difference between anode and cathode rises. Practically, this problem can be avoided by equalizing anode and cathode pressures, to protect the fuel cell from permanent damage. This paper focuses on pressure regulation in the anode and cathode sides of the PEMFC. The control objective is achieved using second order sliding mode multi-input multi-output (MIMO) controller based on “Twisting algorithm”. Parametric uncertainty is formally presented and included in a nonlinear dynamic fuel cell model. The resultant nonlinear controller is robust and is proved to guarantee performance around any equilibrium point and under parametric uncertainty. Simulation results show that the proposed controller has a good transient response under load variations. 相似文献
80.
Ralph Gilles Michael Hofmann Yan Gao Frank Johnson Luana Iorio Michael Larsen Feng Liang Markus Hoelzel Bruno Barbier 《Metallurgical and Materials Transactions A》2010,41(5):1144-1150
The effects of tailoring FeCo alloys with addition of ternary elements Pt, Pd, Mn, Ir, and Re have been investigated. In the
composition range of 30 to 70 pct Co, FeCo alloys undergo a continuous order-disorder phase transition at a maximum temperature
of 730 °C at the equiatomic composition. The effects of temperature and composition on the degree of long-range order in six
alloys (Fe70Co30, Fe67Co30Pt3, Fe67Co30Pd3, Fe67Co30Mn3, Fe67Co30Ir3, and Fe67Co30Re3; at. pct) were compared using neutron diffraction. The transition goes along with growing of antiphase domains during the
heating process. 相似文献