Tracking individual kinesin motors in living cells using single quantum-dot imaging

We report a simple method using semiconductor quantum dots (QDs) to track the motion of intracellular proteins with a high sensitivity. We characterized the in vivo motion of individual QD-tagged kinesin motors in living HeLa cells. Single-molecule measurements provided important parameters of the motor, such as its velocity and processivity, as well as an estimate of the force necessary to carry a QD. Our measurements demonstrate the importance of single-molecule experiments in the investigation of intracellular transport as well as the potential of single quantum-dot imaging for the study of important processes such as cellular trafficking, cell polarization, and division.     

TEM observations and finite element modelling of channel deformation in pre-irradiated austenitic stainless steels - Interactions with free surfaces and grain boundaries

Transmission electron microscopy (TEM) observations show that dislocation channel deformation occurs in pre-irradiated austenitic stainless steels, even at low stress levels (∼175 MPa, 290 °C) in low neutron dose (∼0.16 dpa, 185 °C) material. The TEM observations are utilized to design finite element (FE) meshes that include one or two “soft” channels (i.e. low critical resolved shear stress (CRSS)) of particular aspect ratio (length divided by thickness) embedded at the free surface of a “hard” matrix (i.e. high CRSS). The CRSS are adjusted using experimental data and physically based models from the literature. For doses leading to hardening saturation, the computed surface slips are as high as 100% for an applied stress close to the yield stress, when the observed channel aspect ratio is used. Surface slips are much higher than the grain boundary slips because of matrix constraint effect. The matrix CRSS and the channel aspect ratio are the most influential model parameters. Predictions based on an analytical formula are compared with surface slips computed by the FE method. Predicted slips, either in surface or bulk channels, agree reasonably well with either atomic force microscopy measures reported in the literature or measures based on our TEM observations. Finally, it is shown that the induced surface slip and grain boundary stress concentrations strongly enhance the kinetics of the damage mechanisms possibly involved in IASCC.     

Statistical prioritization for software product line testing: an experience report

Software product lines (SPLs) are families of software systems sharing common assets and exhibiting variabilities specific to each product member of the family. Commonalities and variabilities are often represented as features organized in a feature model. Due to combinatorial explosion of the number of products induced by possible features combinations, exhaustive testing of SPLs is intractable. Therefore, sampling and prioritization techniques have been proposed to generate sorted lists of products based on coverage criteria or weights assigned to features. Solely based on the feature model, these techniques do not take into account behavioural usage of such products as a source of prioritization. In this paper, we assess the feasibility of integrating usage models into the testing process to derive statistical testing approaches for SPLs. Usage models are given as Markov chains, enabling prioritization of probable/rare behaviours. We used featured transition systems, compactly modelling variability and behaviour for SPLs, to determine which products are realizing prioritized behaviours. Statistical prioritization can achieve a significant reduction in the state space, and modelling efforts can be rewarded by better automation. In particular, we used MaTeLo, a statistical test cases generation suite developed at ALL4TEC. We assess feasibility criteria on two systems: Claroline, a configurable course management system, and Sferion?, an embedded system providing helicopter landing assistance.     

Thermoelectric transport in graphene and 2D layered materials

ABSTRACT

In early 90s, Hicks and Dresselhaus proposed that low dimensional materials are advantages for thermoelectric applications due to the sharp features in their density-of-states, resulting in a high Seebeck coefficient and, potentially, in a high thermoelectric power factor. Two-dimensional (2D) materials are the latest class of low dimensional materials studied for thermoelectric applications. The experimental exfoliation of graphene, a single-layer of carbon atoms in 2004, triggered an avalanche of studies devoted to 2D materials in view of electronic, thermal, and optical applications. One can mix and match and stack 2D layers to form van der Waals hetero-structures. Such structures have extreme anisotropic transport properties. Both in-plane and cross-plane thermoelectric transport in these structures are of interest. In this short review article, we first review the progress achieved so far in the study of thermoelectric transport properties of graphene, the most widely studied 2D material, as a representative of interesting in-plane thermoelectric properties. Then, we turn our attention to the layered materials, in their cross-plane direction, highlighting their role as potential structures for solid-state thermionic power generators and coolers.     

Human diffusion and city influence

Cities are characterized by concentrating population, economic activity and services. However, not all cities are equal and a natural hierarchy at local, regional or global scales spontaneously emerges. In this work, we introduce a method to quantify city influence using geolocated tweets to characterize human mobility. Rome and Paris appear consistently as the cities attracting most diverse visitors. The ratio between locals and non-local visitors turns out to be fundamental for a city to truly be global. Focusing only on urban residents'' mobility flows, a city-to-city network can be constructed. This network allows us to analyse centrality measures at different scales. New York and London play a central role on the global scale, while urban rankings suffer substantial changes if the focus is set at a regional level.     

Work Extrinsic and Intrinsic Motivation Scale: Its value for organizational psychology research.

[Correction Notice: An erratum for this article was reported in Vol 42(1) of Canadian Journal of Behavioural Science/Revue canadienne des sciences du comportement (see record 2009-25142-008). In this article, line 2 of the second table was missing from the printed article. The correct table is reprinted in this correction.] The Work Extrinsic and Intrinsic Motivation Scale (WEIMS) is an 18-item measure of work motivation theoretically grounded in self-determination theory (Deci & Ryan, 2000). The purpose of the present research was twofold. First, the applicability of the WEIMS in different work environments was evaluated. Second, its factorial structure and psychometric properties were assessed. Two samples of workers (military: N = 465; civilians: N = 192) voluntarily completed questionnaires. Using the WEIMS’s 3 indexes (work self-determination index, work self-determined and nonself-determined motivation, respectively), results of regression analyses were supportive of its ability to predict positive and negative criteria in the workplace. Results also showed the adequacy of both its construct validity and internal consistency. Its factorial structure was also invariant across samples. Finally, its quasi-simplex pattern and relationships with psychological correlates further supported the self-determination continuum. Overall, these findings provide evidence for the applicability as well as the reliability and validity of the WEIMS in organisational settings. Results are discussed in regard to the applicability of self-determination theory to the workplace. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Attenuated total reflection infrared spectroscopy: an efficient technique to quantitatively determine the orientation and conformation of proteins in single silk fibers

Polarized attenuated total reflection (ATR) infrared spectroscopy is an efficient technique to determine the orientation and conformation of a large variety of samples, but it is more difficult to apply to very small specimens such as silk fibers. The Golden Gate single-reflection ATR accessory that uses diamond as an ATR element and a focalized beam turns out to be highly efficient to study quantitatively the orientation and conformation of a single silk fibroin filament of the silkworm Bombyx mori that is about 10 mum in diameter. For orientation measurements, rotating the sample instead of the electric field greatly simplifies the theoretical analysis and keeps the penetration depth of the infrared radiation constant. A sample holder that can be fitted on the ATR accessory has thus been developed to allow accurate rotation of the sample and to obtain spectra with a low, non-damaging, and reproducible pressure on the fiber. To validate the method, spectra have been recorded as a function of the angle theta between the fiber axis and the polarization of the incident radiation. The data have been fitted following the cosine square dependency of the absorbance with respect to the angle theta. The procedure has been applied to the spectral components of the amide I bands, as determined from spectral decomposition. Multiple angle measurements turn out to be quite useful to correct systematic angle errors and validate the accuracy of the curve-fitting parameters of the band decomposition. By using the calculated dichroic ratio, a parameter of -0.46 +/- 0.01 has been calculated for the antiparallel beta-sheets and -0.04 +/- 0.02 for the remaining structures. From the orientation-insensitive spectrum A(0), the amount of beta-sheets has been estimated to 49 +/- 3%. The results obtained from only two measurements with the electric field of the incident radiation parallel and perpendicular to the fiber axis has demonstrated that ATR spectroscopy can be used routinely in quantitative studies of the molecular orientation and conformation of macromolecules.     

Prediction of grain boundary stress fields and microcrack initiation induced by slip band impingement

Slip localization is widely observed in metallic polycrystals undergoing cyclic deformation or post-irradiation tensile deformation, whatever their crystallographic structure. Hence, strong strain localization occurs in thin slip bands (SBs) inducing by the way local stress concentrations at their intersections with grain boundaries (GBs). Many GB stress field formulae based on the dislocation pile-up theory have been proposed since the pionnering work of Stroh and others. These allow the use of the Griffith criterion for prediction GB fracture initiation. However, recent observations show that assuming that slip is localized on a single atomic plane leads to unrealistic results. In fact, a large number of slip planes are plastically activated and then finite slip band thickness should be accounted for. Numerous crystalline finite element (FE) computations have been carried out using considering a slip bands with low critical resolved shear stress embedded in an elastic matrix. The computed GB normal and shear stress fields:

are considerable lower than the pile-up ones and exhibit strong dependency on the slip band thickness close to the SB corner

but are in fair agreement with the solution predicted by the pile-up theory far away.

Since the pile-up theory leads to the overestimation of the local GB stress fields, the main goal of the current paper is to perform analytical model of GB stress components based upon FE calculations. The effect of various parameters can be understood in the framework of matching asymptotic expansions which is usually applied to cracks with V notches of finite thickness. Finally, the predicted remote stresses to GB fracture in pre-irradiated austenitic stainless steels subjected to tensile loading in various environment are compared to experimental data and the pile-up based predictions.     

SIAPhos: Phosphorylated Sulfonimidamides and their Use in Iridium‐Catalyzed Asymmetric Hydrogenations of Sterically Hindered Cyclic Enamides

Phosphorylated sulfonimidamides (SIAPhos) undergo ion exchange reactions with cationic complexes, [Rh(cod)₂BF₄] and [Ir(cod)₂BarF], or neutral complexes [Rh(cod)Cl]₂ and [Ir(cod)Cl]₂, leading to unprecedented neutral complexes with P‐N‐S‐N chelates. Use of the resulting neutral iridium complexes in asymmetric hydrogenation reactions of tri‐ and tetrasubstituted enamides leads to products with high enantioselectivities (up to 92% ee).