High-throughput nuclear magnetic resonance analysis using a multiple coil flow probe

An automated method for high-throughput nuclear magnetic resonance (NMR) spectroscopy has been developed using a four-coil Multiplex NMR probe. The probe is constructed with solenoidal microcoils optimized for detection of small volume, mass-limited samples and a flow-through design. Four samples can be simultaneously injected into the Multiplex probe with a robotics liquid handler and then analyzed in rapid succession using a selective excitation experiment. Due to the simultaneous injection of four samples and the reduced analysis time with rapid selective excitation, the analysis rate achieved thus far is as low as 1 sample/34 s for 1D 1H NMR.     

Immuno-SEM characterization of developing bovine cartilage

Collagen is a vital material in the tissues of living organisms. Found almost everywhere in the human body, collagen is important in connective tissues, bone growth, and cartilage. Collagen XI makes up a very small portion of the cartilaginous tissue; however, it plays a key role in cartilaginous tissue. Collagen XI and two collagen XI isoforms, V1b and V2, are critical in the ossification process. The location of collagen XI, V1b, V2, and their specific functions in the ossification process within developing bovine cartilage are not well characterized. In this work, the location of collagens I, II, XI and two collagen XI isoforms, V1b and V2, present in developing bovine cartilage are investigated using the immuno-SEM technique. The results for the locations of collagen I and II indicate a high level of consistency with previous work, thus showing that the technique of immuno-SEM can be used with confidence to determine the location of various collagen types within cartilaginous and mineralized tissue. This work has shown that collagen XI is present in the lower hypertrophic region and also in a pericellular arrangement, within about two microns of cell walls, throughout the cartilaginous tissue. V1b is expressed in the articular surface, mineralized region, resting zone, and the distal edge of the diaphysis. The V2 isoform is most strongly expressed in areas of newly forming cartilage, and disappears with chondrocyte maturation. V2 is present in the distal edge of the epiphysis, as well as in mineralized tissue. Collagen XI and two of its isoforms, V1b and V2, are thought to play a critical role in the ossification process. However, this role is not well understood, and is still being characterized. The detection of collagen XI and two of its isoforms in the osteo-chondral junction as well as at a joint surface further point to collagen XI, V1b, and V2 playing a vital role in the ossification process, and warrants further research as to their specific function within the ossification process.     

Social control in personal relationships: Impact on health behaviors and psychological distress.

This study examines associations among social control, health behavior change, and psychological distress. Social control refers to interactions between social network members that entail regulation, influence, and constraint. Social control is predicted to have dual effects leading to better health practices while also arousing psychological distress. A random sample of 242 individuals answered questions about health practices, overall exposure to social control in their networks, and social control attempts made by a specific network member. Analyses yielded mixed support for the hypotheses, but analyses of responses to the social control attempts of a specific network member did reveal that social control predicted less health-compromising behavior and more health-enhancing behavior as well as more distress. The results suggest that social control warrants greater attention in efforts to understand how personal relationships influence health. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Correlates of motorcycle helmet use among recent graduates of a motorcycle training course

Helmets significantly decrease morbidity and mortality from motorcycle crashes, but many areas of the world lack universal helmet laws. To educate motorcyclists in areas without helmet laws, more knowledge of motorcyclists’ helmet beliefs is needed.A web-based survey was therefore designed to assess motorcyclists’ attitudes, norms and behaviors towards helmets in a U.S. state with a limited helmet law. Of 445 survey respondents, 68.4% of respondents reported always wearing a helmet. The not-always-helmeted riders were more likely than the always-helmeted to be male; to bave less education; and to have a history of previous motorcycle crashes and injuries. Although both groups had taken rider training classes, fewer of the not-always-helmeted had learned how to ride in a class. The strongest correlates of being not-always-helmeted (vs. always-helmeted) were attitudes that helmets were not protective and impaired sight/hearing; and the normative belief that they would only wear helmets if forced by law. Because attitudes are often more easily changed than normative beliefs, education may increase helmet use. However, less than half of riders in this state with a mandatory education program learned how to ride from a rider education course, and 44% of non-helmeted said they would only wear a helmet if forced by law. Legislation may therefore be a more efficient and effective strategy than education to increase helmet use.     

Pattern-based adaptive process control

A controller that has an adaptation strategy based on the patterns exhibited in the recent history of the controller error and manipulated input variable is detailed. The method of approach is to analyze all controller error transients to extract features including overshoot and damping. The deviation of these features from desired values is then used to update the parameters of an internal controller model. When the error transient is caused by a disturbance, the patterns exhibited in the manipulated input variable are also considered to determine whether the disturbance has actually changed the character of the process, or if the error patterns are simply reflecting the nature of the disturbance itself. The strategy is general to a number of model-based controllers and is demonstrated here using both a long range predictive controller and a PI controller with Smith predictor. Details of the strategy are presented along with a demonstration of the method on process simulations constructed to present challenging applications.     

Children's spatial knowledge of their school campus.

72 4th-, 6th-, and 8th-grade boys were tested with different methodologies for deriving measures of their knowledge of interlandmark distances and directions on their school campus. Results show that age group differences were not large and appeared comparable for most measures. However, other results indicated that measures derived from direction vs distance information were independent. First, the accuracy with which Ss made distance and direction estimates varied across specific combinations of reference sites and targets; this pattern of differential accuracy was quite different for distance vs direction estimates. Second, correlations among the dependent measures indicated that Ss who were accurate at direction estimates were not necessarily accurate for distance estimates. It is emphasized that measures derived from Ss' estimates of direction information were not comparable with measures derived from their estimates of distance information. (19 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Narp regulates long-term aversive effects of morphine withdrawal.

Although long-lasting effects of drug withdrawal are thought to play a key role in motivating continued drug use, the mechanisms mediating this type of drug-induced plasticity are unclear. Because Narp is an immediate early gene product that is secreted at synaptic sites and binds to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, it has been implicated in mediating enduring forms of synaptic plasticity. In previous studies, the authors found that Narp is selectively induced by morphine withdrawal in the extended amygdala, a group of limbic nuclei that mediate aversive behavioral responses. Accordingly, in this study, the authors evaluate whether long-term aversive effects of morphine withdrawal are altered in Narp knockout (KO) mice. The authors found that acute physical signs of morphine withdrawal are unaffected by Narp deletion. However, Narp KO mice acquire and sustain more aversive responses to the environment conditioned with morphine withdrawal than do wild type (WT) controls. Paradoxically, Narp KO mice undergo accelerated extinction of this heightened aversive response. Taken together, these studies suggest that Narp modulates both acquisition and extinction of aversive responses to morphine withdrawal and, therefore, may regulate plasticity processes underlying drug addiction. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Contextual control over conditioned responding in an extinction paradigm.

Four experiments studied contextual control over rats' freezing to conditioned stimuli (CSs) that had been paired with shock and were then extinguished. In Experiment 1, rats were exposed to a CS A–shock and a CS B–shock pairing in Context C. CS A was then extinguished in Context A, and CS B in Context B. Freezing was renewed when each CS was presented in the context where the other CS had been extinguished. In Experiments 2–4, rats were exposed to a CS A–shock pairing in A and a CS B–shock pairing in B. They were then exposed to Context C where one, both, or neither of the CSs were extinguished, or where both CSs continued to be reinforced. On test, the rats froze more to CS A than to CS B in Context A, and more to CS B than to CS A in Context B, but only if the CSs had been extinguished. Thus, after extinction, rats use contexts to regulate retrieval not only of their memory for extinction, but also of their memory for the original conditioning episode. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Multilayer film preparation of poly(4-vinylphenol) from aqueous media

The use of weak polyelectrolytes in multilayer polymer systems provides a means of altering the physico-chemical properties of these thin films. This paper examines the limits of the polyanions by incorporating the extremely weak polyelectrolyte, poly(4-vinylphenol) (PVPh), into a multilayer system with poly(allylamine hydrochloride) (PAH) from a dilute aqueous media. Film growth was monitored using absorption spectroscopy and revealed a clear linear trend in film thickness. The surface morphology, thickness, and swelling of the multilayer films systems were examined using atomic force microscopy (AFM). Film thickness greatly depended upon the pH of the polymer precursor solutions; 50-layer systems created at pH 12.5 and 11.0 were 8.2 and 246.6 nm thick, respectively, a difference of over 2900%. This is the first report on the incorporation of PVPh into a multilayer system from an aqueous media.     

3-Deoxyschweinfurthin B Lowers Cholesterol Levels by Decreasing Synthesis and Increasing Export in Cultured Cancer Cell Lines

The schweinfurthins have potent antiproliferative activity in multiple glioblastoma multiforme (GBM) cell lines; however, the mechanism by which growth is impeded is not fully understood. Previously, we demonstrated that the schweinfurthins reduce the level of key isoprenoid intermediates in the cholesterol biosynthetic pathway. Herein, we describe the effects of the schweinfurthins on cholesterol homeostasis. Intracellular cholesterol levels are greatly reduced in cells incubated with 3‐deoxyschweinfurthin B (3dSB), an analog of the natural product schweinfurthin B. Decreased cholesterol levels are due to decreased cholesterol synthesis and increased cholesterol efflux; both of these cellular actions can be influenced by liver X‐receptor (LXR) activation. The effects of 3dSB on ATP‐binding cassette transporter 1 levels and other LXR targets are similar to that of 25‐hydroxycholesterol, an LXR agonist. Unlike 25‐hydroxycholesterol, 3dSB does not act as a direct agonist for LXR α or β. These data suggest that cholesterol homeostasis plays a significant role in the growth inhibitory activity of the schweinfurthins and may elucidate a mechanism that can be targeted in human cancers such as GBM.