Histone Methylation Marks on Circulating Nucleosomes as Novel Blood-Based Biomarker in Colorectal Cancer

Circulating nucleic acids (CNAs) are under investigation as a liquid biopsy in cancer as potential non-invasive biomarkers, as stable structure in circulation nucleosomes could be valuable sources for detection of cancer-specific alterations in histone modifications. Our interest is in histone methylation marks with a focus on colorectal cancer, one of the leading cancers respective the incidence and mortality. Our previous work included the analysis of trimethylations of lysine 9 on histone 3 (H3K9me3) and of lysine 20 on histone 4 (H4K20me3) by chromatin immuno- precipitation-related PCR in circulating nucleosomes. Here we asked whether global immunologic measurement of histone marks in circulation could be a suitable approach to show their potential as biomarkers. In addition to H3K9me3 and H4K20me3 we also measured H3K27me3 in plasma samples from CRC patients (n = 63) and cancer free individuals (n = 40) by ELISA-based methylation assays. Our results show that of three marks, the amounts of H3K27me3 (p = 0.04) and H4K20me3 (p < 0.001) were significantly lower in CRC patients than in healthy controls. For H3K9me3 similar amounts were measured in both groups. Areas under the curve (AUC) in receiver operating characteristic (ROC) curves indicating the power of CRC detection were 0.620 for H3K27me3, 0.715 for H4K20me3 and 0.769 for the combination of both markers. In conclusion, findings of this preliminary study reveal the potential of blood-based detection of CRC by quantification of histone methylation marks and the additive effect of the marker combination.     

Formation of metastable wurtzite phase boron nitride by emulsion detonation synthesis

Emulsion detonation synthesis (EDS) is a newly developed process to synthesize nano‐sized ceramic powders based on the detonation of 2 water‐in‐oil emulsions. The process provides high pressure and temperature along with rapid quenching. In this work, we report the formation of wurtzite phase BN (w‐BN) for the first time by EDS process, using hexagonal BN (h‐BN) as the precursor. Characterization studies demonstrated the formation of w‐BN with sizes varying from nanometer to micrometer scale either embedded in or grown from h‐BN matrix. These findings provide a new avenue to synthesize metastable and superhard BN phases.     

Dexterous workspace optimization of an asymmetric six-degree of freedom Stewart–Gough platform type manipulator

In this paper, an asymmetric Generalized Stewart–Gough Platform (GSP) type parallel manipulator is designed by considering the type synthesis approach. The asymmetric six-Degree Of Freedom (DOF) manipulator optimized in this paper is selected among the GSPs classified under the name of 6D. The dexterous workspace optimization of Asymmetric parallel Manipulator with tEn Different Linear Actuator Lengths (AMEDLAL) subject to kinematics and geometric constraints is performed by using the Particle Swarm Optimization (PSO). The condition number and Minimum Singular Value (MSV) of homogenized Jacobian matrix are employed to obtain the dexterous workspace of AMEDLAL. Finally, the six-DOF AMEDLAL is also compared with the optimized Traditional Stewart–Gough Platform Manipulator (TSPM) considering the volume of the dexterous workspace in order to demonstrate its kinematic performance. Comparisons show that the manipulator proposed in this study illustrates better kinematic performance than TSPM.     

Effect of microwave radiation on processing characteristics of sulphide minerals in inert atmosphere

The influence of microwave radiation on sulphide minerals in inert atmosphere has been investigated with respect to their processing characteristics such as magnetic susceptibility and floatability. Pure sulphide mineral samples were prepared in different size fractions. Microflotation tests were performed and magnetic susceptibilities of samples were measured after treating with microwave energy in inert atmosphere at three power levels for six time intervals. Possible modifications on the mineral surfaces due to microwave energy and exposure time were evaluated by XRD and optical microscopy. The results showed that selective separation of sulphide minerals in terms of floatability and magnetic separation could be possible.     

Parasitic compensation in CCI-based circuits for reduced power consumption

In this paper, the compensation advantage of the first-generation current conveyor (CCI) over the second-generation current conveyor (CCII) in tunable circuits is shown. For this purpose, a new floating frequency dependent negative resistor (FDNR) simulator using three CCIs is presented and employed in a third-order high-pass filter. The compensation feature of the CCI is shown for the proposed high-pass filter. As a second example, the presented compensation method is tested in a second-order band-pass filter constructed with two CCIs. Applying the proposed compensation technique, the CCI-based circuits can operate in lower biasing currents, which result in lower power consumption.     

Planning of capacity,production and inventory decisions in a generic reverse supply chain under uncertain demand and returns

There is a growing interest for the design and operation of reverse supply chain systems due to the cost and the legislation issues. In this paper, we address the disassembly, refurbishing and production operations in a reverse supply chain setting for modular products such as computers and mobile phones considering the uncertainties in this system, which are the return amounts of the used products and demand for final products. We develop a large-scale mixed integer programming model in order to capture all characteristics of this system, and use two-stage stochastic optimisation and robust optimisation approaches to analyse the system behaviour. In the first stage, we focus on the strategic decisions about the capacities at disassembly and refurbishing sites considering different scenarios regarding the uncertainties in the system. In the second stage, we analyse the operational decisions such as production, inventory and disposal rates. We observe through our extensive numerical analysis that the randomness of demand and return values effect the performance of the system substantially and the uncertainty of the return amounts of used products is much more important than the uncertainty of demand in this system.     

Process control at the sealing line during sheet metal hydroforming

Sheet metal hydroforming is a deep drawing process that uses a pressure medium for load transmission. The process is mainly influenced by the control of the blank holder force and the fluid pressure. This report describes investigations of a newly developed process-control system. Process variables can be controlled by the sealing state of the tool. The detection is performed by a CCD-camera system that allows the continuous recording of occurring leakages. Different control strategies can be used to control the multiple process parameters. This paper describes the performance and the limits of the aforementioned technology.     

Enhancing electrochromic properties of polypyrrole by silsesquioxane nanocages

A new monomer, octa(thiophenephenyl)silsesquioxane (OThiophenePS) was synthesized via click chemistry. The chemical structure of OThiophenePS was characterized by nuclear magnetic resonance (¹H NMR) and Fourier transform infrared (FT-IR) spectroscopies. Electrochemical polymerization of pyrrole with OThiophenePS was performed resulting in polypyrrole-attached, polyhedral oligomeric silsesquioxane (OPS–PPy). The spectroelectrochemical studies show that the electrochromic properties of (OPS–PPy) are superior to those of polypyrrole (PPy). This great improvement can be attributed to the more accessible doping sites and the facile ion movement during the redox switching brought by the loose packing of the PPy chains.     

IGNITION DELAY CHARACTERISTICS OF SOME TURKISH VEGETABLE OIL-DIESEL FUEL BLENDS

Vegetable oils have chances to be used in Diesel engines as alternative fuels contributing to the solution of some agricultural, environmental and economical problems. Direct use of them has some technical problem yet but as blended fuels with diesel fuel or esters they have places on the application area. In this paper the effect of the compression ratio on ignition delay is investigated in an ASTM-CER engine working with four different types of vegetable oil of Turkish origin (sunflower, corn, soybean, and olive oil) blended with grade No.2-D diesel fuel at a ratio of 20/80 (v/v) and the results are compared with baseline diesel fuel. Longer ignition delay periods have generally been obtained for blend fuels ranking from olive oil to sunflower oil as compared to diesel fuel.