Validation and modification of modeling thermally activated building systems (TABS) using EnergyPlus

EnergyPlus (EP) integrates a low temperature radiant system module to evaluate thermal performance of radiant systems such as thermally activated building systems (TABS), but the assumptions in this module neglect thermal resistance of the pipe and thermal resistance between the pipe exterior surface and the pipe level, which may result in the inaccurate evaluation of TABS in terms of surface temperature and surface heat flow. In this paper, in order to validate this module used in EP, steady and transient heat transfer processes of TABS in buildings were studied by analytical solution, two-dimensional numerical simulation and EP simulation. The comparison shows that the assumptions indeed result in a largely overestimated cooling and heating capacity of TABS. In order to improve this radiant module, a simple solution of introducing a no mass material layer with the neglected thermal resistances to both sides of the pipe level was proposed. With this method, the results of mean surface temperature and mean heat flow show good agreement with that from analytical solution as well as numerical simulation. Furthermore, the results of the simulation coupling the modified module with room systems show very small deviation from the results found in the literature. In addition, the application of the modified module in a hollow core concrete deck structure with TABS was investigated.     

Assessing cellular toxicities in fibroblasts upon exposure to lipid-based nanoparticles: a high content analysis approach

Lipid-based nanoparticles (LNPs) are widely used for the delivery of drugs and nucleic acids. Although most of them are considered safe, there is confusing evidence in the literature regarding their potential cellular toxicities. Moreover, little is known about the recovery process cells undergo after a cytotoxic insult. We have previously studied the systemic effects of common LNPs with different surface charge (cationic, anionic, neutral) and revealed that positively charged LNPs ((+)LNPs) activate pro-inflammatory cytokines and induce interferon response by acting as an agonist of Toll-like receptor 4 on immune cells. In this study, we focused on the response of human fibroblasts exposed to LNPs and their cellular recovery process. To this end, we used image-based high content analysis (HCA). Using this strategy, we were able to show simultaneously, in several intracellular parameters, that fibroblasts can recover from the cytotoxic effects of (+)LNPs. The use of HCA opens new avenues in understanding cellular response and nanotoxicity and may become a valuable tool for screening safe materials for drug delivery and tissue engineering.     

Tight and uniform layer of covalently bound aminoethylophenyl groups perpendicular to gold surface for attachment of biomolecules

Strongly adhered layers of the compound with the primary amino group directed toward the solution were obtained at the gold surface by chronoamperometric electroreduction of 4-aminoethylobenzenodiazonium salt (AEBD) in acetonitrile solution at appropriately selected potential. The used techniques (EQCM, AFM, EIS, PM, IRRAS) showed that the nature and thickness of formed aminoethylophenyl layer strongly depend on the potential applied to the electrode. Electroreduction of AEBD salt at a potential more negative than -0.6 V (vs Ag/AgCl) leads to about monolayer on the gold surface. Additionally, such a layer was very tight and uniform. The electrochemical measurements indicate that the efficient and precise attachment of biomolecules to the aminoethylophenyl layer is only possible when this layer is formed at appropriate potential. This was shown for ss- and dsDNA.     

Fast point-based 3-D alignment of live cells.

Typical time intervals between acquisitions of three-dimensional (3-D) images of the same cell in live cell imaging are in the orders of minutes. In the meantime, the live cell can move in a water basin on the stage. This movement can hamper the studies of intranuclear processes. We propose a fast point-based image registration method for the suppression of the movement of a cell as a whole in the image data. First, centroids of certain intracellular objects are computed for each image in a time-lapse series. Then, a matching between the centroids, which have the maximal number of pairs, is sought between consecutive point sets by a 3-D extension of a two-dimensional fast point pattern matching method, which is invariant to rotation, translation, local distortion, and extra/missing points. The proposed 3-D extension assumes rotations only around the z axis to retain the complexity of the original method. The final step involves computing the optimal fully 3-D transformation between images from corresponding points in the least-squares manner. The robustness of the method was evaluated on generated data. The results of the simulations show that the method is very precise and its correctness can be estimated. This article also presents two practical application examples, namely the registration of images of HP1 domains and the registration of images of telomeres. More than 97% of time-consecutive images were successfully registered. The results show that the method is very well suited to live cell imaging.     

Enhancing nitrification in vertical flow constructed wetland utilizing a passive air pump

Nitrification of simulated secondary effluent solution using a variant of the vertical flow bed constructed wetland was studied. In this system oxygen required for the nitrification process is supplied by a passive air pump. The passive air pump is based on a fill and draw cycle, where oxygen depleted air is removed from the system while fresh air is introduced. Each volume of effluent drained is displaced by an equal volume of fresh air. Spatial and temporal oxygen distribution in the system as a function of drained effluent volume was investigated. Experimental results of nitrification and the corresponding oxygen consumption show good agreement with theoretical calculations based on physico–chemical considerations. Observations show that while oxygen distribution within the media was found to be non-uniform at the beginning of each cycle, it approached more uniform distribution with time. The latter resulted mainly from diffusion of oxygen in the gaseous phase.     

Deposition of aerosol (“hygroscopic dust”) on electronics – Mechanism and risk

In this paper the risk associated with deposition of aerosol (“hygroscopic dust”) on printed circuit boards is assessed for different equipment designs and deployment. The amount of deposited dust is related to (1) the total dust availability in a given type of equipment and (2) to the rate of deposition of dust due to different mechanisms. Of these, the Brownian diffusion, gravitational settling, thermophoresis and electrophoresis are of different relative importance depending on such design factors as air flow in the enclosure, temperature gradients between boards and walls and presence of electric fields. The expressions for electric fields in the vicinity of printed circuit boards relevant to electrophoretic deposition are derived. It is shown that not only the sealed Absolute Humidity Control Modules but also the breathing Relative Humidity Control Modules should not present a hygroscopic dust risk for either indoor or outdoor applications. Direct air cooled system with forced convection present a definite dust reliability risk both for indoor and outdoor applications while open natural convection cooled systems present a borderline case where each design has to be considered separately.     

Computing occluding and transparent motions

Computing the motions of several moving objects in image sequences involves simultaneous motion analysis and segmentation. This task can become complicated when image motion changes significantly between frames, as with camera vibrations. Such vibrations make tracking in longer sequences harder, as temporal motion constancy cannot be assumed. The problem becomes even more difficult in the case of transparent motions.A method is presented for detecting and tracking occluding and transparent moving objects, which uses temporal integration without assuming motion constancy. Each new frame in the sequence is compared to a dynamic internal representation image of the tracked object. The internal representation image is constructed by temporally integrating frames after registration based on the motion computation. The temporal integration maintains sharpness of the tracked object, while blurring objects that have other motions. Comparing new frames to the internal representation image causes the motion analysis algorithm to continue tracking the same object in subsequent frames, and to improve the segmentation.     

Pinning of Austenite Grain Boundaries by

The growth behavior of austenite grains in the presence of A1N precipitates varies with the temperature and time of anneal. To study this behavior, two iron alloys, (in weight percent) a 0.1 carbon base chemistry with 0.03A1/0.01N and 0.09A1/0.04N, respectively, were annealed between 1000 °C and 1200 °C for times of up to 180 minutes. Using optical microscopy, as many as 1000 austenite grains per heat-treatment condition were measured. Conditions of sup- pressed, abnormal, and uniform grain growth were observed. Using an extraction replica tech- nique, the size, shape, and distribution of the A1N particles were determined using transmission electron microscopy (TEM). The largest grain boundary curvatures calculated, using the Hellman- Hillert pinning model, were in close agreement with independent calculations of curvatures using the grain size data. The largest grains in the lognormal size distribution of austenite grains were found to be the ones with the potential to grow to abnormally large sizes.