DNA-directed immobilization of horseradish peroxidase onto porous SiO2optical transducers

ABSTRACT: Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of Horseradish Peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-Perot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-Directed Immobilization. Preliminary studies demonstrate high enzymatic activity levels of the immobilized Horseradish Peroxidase, while maintaining its specificity. The catalytic activity of the enzymes immobilized within the porous nanostructure is monitored in real time by reflective interferometric Fourier transform spectroscopy. We show that we can easily regenerate the surface for consecutive biosensing analysis by mild de-hybridization conditions.     

Particle swarm optimization algorithm driven by multichaotic number generator

In this paper, the utilization of different chaotic systems as pseudo-random number generators (PRNGs) for velocity calculation in the PSO algorithm are proposed. Two chaos-based PRNGs are used alternately within one run of the PSO algorithm and dynamically switched over when a certain criterion is met. By using this unique technique, it is possible to improve the performance of PSO algorithm as it is demonstrated on different benchmark functions.     

Hydrophobicity of Thin Films of Compounds of Low‐Electronegativity Metals

Hydrophobic materials capable of withstanding harsh conditions are required for various applications. Here, we show that oxides and nitrides of various low‐electronegativity metals are hydrophobic hard ceramics. We attribute their hydrophobicity to low Lewis acidity of the low‐electronegativity cations implying a low ability of the cations on the surface to form coordinate bonds with water oxygen anions. Furthermore, we observe a systematically stronger hydrophobic behavior of nitrides compared with the corresponding oxides, which we attribute to nitrogen being a poorer Lewis base than oxygen due to a reduced number of lone pairs of electrons, implying a lower ability of nitrogen anions on the surface to form hydrogen bonds with water hydrogen cations. Most of the oxides and nitrides investigated exhibit high values of hardness. Therefore, oxides and nitrides of low‐electronegativity metals should find application as hydrophobic materials in harsh conditions.     

The good,the bad and the ugly – three approaches to management of human waste in a high-mountain environment

Each year, millions of mountaineers visit high-mountain areas. They generate tonnes of faeces and cubic hectometres of urine annually. Thus, proper disposal of human waste is important for the conservation and appropriate management of high-mountain areas. The management can address the issue in three ways: the good (complete/non-invasive); the bad (partial/superficial); and the ugly (invasive). With use of those categories, 20 selected summits from different parts of the world have been evaluated, separately in respect to faeces and urine. It was expected that correct or incorrect disposal of human waste would depend on the changing altitude and/or development level. Disappointingly, the correlation between selection criteria (better or worse solution) and the increase of altitude does not exist. Similarly, the increase of the development level does not play a significant role, especially when urine is taken under consideration. The problem is more global than was thought. The paper makes recommendations which could lead to reduction of this problem.     

Enhanced analytical method for IP mobility handover schemes cost evaluation

Seamless service delivery for mobile users complemented with Quality of Service provisioning for their real-time applications have a hot topic in the field of mobile communication in recent years. Seamless mobility goes hand in hand with Mobile IPv6 protocol. Since many different handover schemes trying to solve the Quality of Service issues have been developed a need for means for comparison has arisen. This paper presents an enhanced universal analytical method for comparison of handover schemes. The method focuses on two important aspects influencing the handover performance—binding update cost and packet delivery cost. The use of the proposed method is presented for comparison of four most common handover schemes—MIPv6, HMIPv6, FMIPv6 and F-HMIPv6.     

Hsp100 Molecular Chaperone ClpB and Its Role in Virulence of Bacterial Pathogens

This review focuses on the molecular chaperone ClpB that belongs to the Hsp100/Clp subfamily of the AAA+ ATPases and its biological function in selected bacterial pathogens, causing a variety of human infectious diseases, including zoonoses. It has been established that ClpB disaggregates and reactivates aggregated cellular proteins. It has been postulated that ClpB’s protein disaggregation activity supports the survival of pathogenic bacteria under host-induced stresses (e.g., high temperature and oxidative stress), which allows them to rapidly adapt to the human host and establish infection. Interestingly, ClpB may also perform other functions in pathogenic bacteria, which are required for their virulence. Since ClpB is not found in human cells, this chaperone emerges as an attractive target for novel antimicrobial therapies in combating bacterial infections.     

Novel inorganic precursors [Co4.32Zn1.68(HCO2)18(C2H8N)6]/SiO2 and Co4.32Zn1.68(HCO2)18(C2H8N)6]/Al2O3 for Fischer–Tropsch synthesis

The silica- and alumina-supported Co–Zn catalysts were synthesized by thermal decomposition of new inorganic precursors [Co_4.32Zn_1.68(HCO₂)₁₈(C₂H₈N)₆]/SiO₂ or Al₂O₃. A novel coordination polymer formulated as [Co_4.32Zn_1.68(HCO₂)₁₈(C₂H₈N)₆] (1) was prepared using the solvothermal technique and characterized by elemental analysis, FT-infrared spectroscopy. Thermal stability of the complex 1 was investigated by thermogravimetric analysis and differential scanning calorimetry, and its structure was determined by single-crystal X-ray diffraction. Characterization of catalysts was carried out using powder X-ray diffraction (XRD), scanning electron microscopy (SEM), and BET specific surface area. The catalysts were evaluated for Fischer–Tropsch synthesis (FTS) in the temperature range 200–300 °C. The results revealed that the synthesized catalysts have higher selectivity to the desired products at 260 °C. The performance of the catalysts was compared to those of catalysts constructed via impregnation method and the fabricated catalysts show higher activity and selectivity than the reference catalysts.