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51.
Mike Lafferty Mark J. Dycaico 《Journal of The Association for Laboratory Automation》2004,9(4):200-208
Recombinant approaches for tapping into the biodiversity present in nature for the discovery of novel enzymes and biosynthetic pathways can result in large gene libraries. Likewise, laboratory evolution techniques can result in large but potentially valuable libraries. Thorough screening of these libraries requires ultra high-throughput methods. The GigaMatrix™ screening platform addresses this opportunity using reusable high-density plates with 100,000 to 1,000,000 through-hole wells in a microplate footprint. In addition to throughputs of over 107 wells per day, the platform offers a significant reduction in reagent use and waste, has fully integrated automated “cherry picking,” and uses no complicated dispensing equipment. Wells containing putative hits from targeted fluorescent liquid phase assays are revealed by a fluorescent imaging system. Vision-guided robotics are utilized to recover hits by accessing individual 200 μm and smaller wells with a disposable sterile needle. The GigaMatrix platform has proven to be an effective and efficient tool for screening gene libraries for both discovery and evolution applications. 相似文献
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Definition of the problem
The concept of “ethics” has recently been incorporated into both the 2014 Model Professional Code for Dentists and the current version of the “National Competence-Based Catalog of Learning Objectives in Dental Medicine” (NKLZ, Nationaler Kompetenzbasierter Lernzielkatalog Zahnmedizin). The study presented here addresses the question which relevance this has for the relevance of the new concept accomplished for the dental profession and society.Arguments
To this end, the societal processes that are related to the autonomous freedom of action of the dental profession are discussed. Therefore the sociological fields of discourse such as profession (and the effect of its constitutive antinomies), trust, education, expertise, practice of treatments and sanction are reviewed for their potential of having connections to each other and serving as a structural frame concerning an ethically reflected acting of the professional. The basis of the professional freedom of action is the trust of society and the individual patient in the professionally acting dentist.Conclusion
The study arrives at the conclusion that it was a serious omission for many decades not to make young dentists aware of the ethical foundations of dental activity on an institutionally established basis and thus failing to encourage them to constantly reflect their professional expertise in relation to the problems to be solved. This deficit has now been identified. It is absolutely essential to implement an institutionalized structure dealing with ethical questions within the scope of dental medicine.53.
54.
Shahram Izadi Mike Fraser Steve Benford Martin Flintham Chris Greenhalgh Tom Rodden Holger Schnädelbach 《Personal and Ubiquitous Computing》2002,6(4):290-298
The Citywide project is exploring ways in which technology can provide people with rich and engaging digital experiences
as they move through physical space, including historical experiences, performances and games. This paper describes some initial
results and experiences with this project based upon two prototype demonstrators. In the first, we describe an application
in which a search party explores an archaeological site, uncovering enacted scenes within the virtual world that are of a
historical relevance to their particular physical location. In the second, we describe a museum experience where participants
explore an outdoors location, hunting for buried virtual artifacts that they then bring back to a museum for a more detailed
study. Our demonstrators employ a varied set of devices, including mobile wireless interfaces for locating hotspots of virtual
activity when outdoors, to give different experiences of the virtual world depending upon location, task, available equipment
and accuracy of tracking. We conclude by discussing some of the potential advantages of using an underlying shared virtual
world to support interactive experiences across extended physical settings. 相似文献
55.
Long-term aging of copper added to soils 总被引:3,自引:0,他引:3
Ma Y Lombi E Oliver IW Nolan AL McLaughlin MJ 《Environmental science & technology》2006,40(20):6310-6317
Aging refers to the processes by which the mobility and bioavailability/toxicity of metals added to soil decline with time. Although long-term aging is a key process that needs to be considered in the risk assessment of metals in field soils, the long-term aging of Cu added to soils has not been studied in detail. In this study, the effects of aging on Cu isotopic exchangeability, total Cu in soil pore water, pore water free Cu2+ activity, and "available" Cu measured by the technique of diffusive gradient in thin film (DGT-Cu) were investigated in 19 European soils at two total Cu concentrations shown to inhibit plant (tomato) growth by 10 and 90%. After addition of Cu, the soils were leached, incubated outdoors, and sampled regularly over a 2-year period. The results showed that when water soluble Cu was added to soils, concentrations of Cu determined by each of the techniques tended to decrease rapidly initially, followed by further decreases at slow rates. Soil pH was a vital factor affecting the aging rate of Cu added to soils. The relatively low solubility products and low isotopic exchangeabilities of Cu in calcareous soils immediately after addition of soluble Cu2+ suggested Cu2+ probably precipitated in these soils as Cu2(OH)2CO3 (malachite) and Cu(OH)2. Isotopic dilution was found to be a robust technique for measuring rates of long-term aging reactions. A semi-mechanistic model was developed to describe the rate and extent of Cu aging across soils as affected by soil pH and other physicochemical parameters. Although not measured directly, it is inferred from soil physicochemical controls on Cu aging that processes of precipitation/nucleation of Cu in soils and hydrolysis of Cu2+ followed by a diffusion process controlled the decrease in Cu availability with time. The model was validated by testing it against field soils with different contamination histories and was found to successfully predict the isotopic exchangeability of Cu added to soils based on two parameters: soil pH and time. 相似文献
56.
Mike Yonker 《世界电子元器件》2006,(10):18-20,22
概述
自从20世纪40年代黑白广播电视问世以来,电视已走过了一个漫长的发展历程。我们经历了彩色电视与数字广播电视在画质方面的创新,以及在信号传输方式上的不断进步,如从有线到卫星再到HD等。每项新技术的推出都使电视的受欢迎程度与日俱增,并都能为观众带来更为丰富的节目内容。目前,电视正在迎来它的另一个全新的发展阶段:移动数字电视。 相似文献
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Scientometrics - The lack of females in many Science Technology, Engineering and Mathematics (STEM) subjects in the USA is an ongoing concern, with many initiatives attempting to redress this... 相似文献