Laser transfer processing of thick film lead zirconate titanate and removal of damage layer

A mechanical polishing technique is demonstrated for the effective removal of the interfacial region of a lead zirconate titanate thick film after laser transfer from a sapphire fabrication substrate. The laser-exposed interface originally in contact with sapphire, sintered at 1150 °C, was glass-like in appearance, consistent with melting being the principal de-lamination mechanism during a 15-ns single pulse of KrF laser radiation. The mechanical removal of the laser-generated, amorphous low-permittivity layer resulted in a decrease of the coercive field, from ~45 to ~30 kV/cm, with an increase in relative permittivity (100 Hz) from 350 to 780. Remnant polarisation was ~20 μC/cm² in both sample types. The contribution of the laser-generated damage layer is modelled using a series two-capacitor circuit.     

Modelling internal migration in Kenya: an econometric analysis with limited data

"In this paper the determinants of internal migration in Kenya are analyzed on the basis of a human capital model. Explanatory variables included in the specification are both economic (wage rates and employment rates) and noneconomic (for example, population density and educational attainment). Also incorporated are variables which reflect intervening opportunities.... The econometric results show that destination variables are important determinants of internal migration, as is distance between the districts. Further, the variables for the intervening opportunities add significantly to the explanatory power of the model."     

Cassettes for PCR-mediated gene tagging in Candida albicans utilizing nourseothricin resistance

In recent years a number of molecular tools have been reported for use in the human fungal pathogen Candida albicans, including PCR-mediated approaches for gene disruption, conditional expression and epitope tagging. Traditionally these methods have utilized auxotrophic markers; however, the availability of auxotrophic markers can be limiting and in some instances their use may also impact on the interpretation of results. As a result, the use of positive selection markers has now become more commonplace. Here we report the development and validation of a set of cassettes for PCR-mediated gene tagging and overexpression studies utilizing the nourseothricin resistance (CaNAT1) positive selection marker. In particular we have produced cassettes containing yeast-enhanced GFP, YFP, CFP, RFP and a combined V5-6xHis epitope tag. The cassettes are engineered for use in PCR-mediated gene tagging strategies where insertion is targeted to the 3' end of the gene of interest. In addition, to facilitate protein functional analysis and genetic suppression studies through the use of overexpression, we have also constructed a promoter replacement cassette containing the ENO1 promoter which is known to be expressed at a high level. These cassettes expand on the range of molecular tools available for working with C. albicans and may also be used in other Candida species that display sensitivity to nourseothricin.     

Heartwood of Cupressus lusitanica, C. macrocarpa, Leyland and Ovens cypress and prediction of its durability using near-infrared spectroscopy

The heartwood of plantation-grown Cupressus lusitanica, C. macrocarpa, Leyland and Ovens cypress (×Cuprocyparis leylandii and ×C. ovensii) from trials in New Zealand, was evaluated using breast height increment cores and in vitro decay tests with fungal cultures to determine the variation in the heartwood content and natural durability, and its prediction using near-infrared (NIR) spectroscopy. The increment core measurements showed the length of the cores was strongly influenced by the stocking and site, but the heartwood content and weight loss with decay testing were strongly influenced by species and genotype. The heartwood content was consistently high for C. macrocarpa, but varied widely for C. lusitanica, and the Leyland and Ovens cypress clones. The weight loss was similar for the species and cypress clones, with the heartwood classified as very durable and durable, but there were differences in the distributions of less durable heartwood. The NIR calibration models of mass loss with fungal decay testing had ratios of prediction to deviation (RPD) of 1.0–1.3, which suggests the models could be used to segregate the heartwood for high and low values of natural durability.     

On-chip deposition of carbon nanotubes using CMOS microhotplates

The direct deposition of carbon nanotubes on CMOS microhotplates is demonstrated in this paper. Tungsten microhotplates, fabricated on thin SOI membranes aside CMOS control circuitry, are used to locally grow carbon nanotubes by chemical vapour deposition. Unlike bulk heating of the entire chip, which could cause degradation to CMOS devices and interconnects due to high growth temperatures in excess of 500?°C, this novel technique allows carbon nanotubes to be grown on-chip in localized regions. The microfabricated heaters are thermally isolated from the rest of the CMOS chip as they are on the membranes. This allows carbon nanotubes to be grown alongside CMOS circuitry on the same wafer without any external heating, thus enabling new applications (e.g.?smart gas sensing) where the integration of CMOS and carbon nanotubes is required.     

Recent advances in the biochemistry and clinical relevance of the isoprostane pathway

Isoprostanes (IsoPs), lipid peroxidation products formed via the free radical-mediated oxidation of arachidonic acid, have become the “gold standard” biomarker of oxidative stress in vivo over the past 15 yr. Significant advances have been made in understanding this important pathway of lipid peroxidation. Recent studies from our laboratory are discussed that have provided insights into the mechanism of formation and regioisomeric distribution of these compounds and that have identified novel products of the IsoP pathway such as cyclized dioxolane IsoPs, IsoP-derived racemic prostaglandins, and reactive cyclopentenone IsoP, the latter of which possess potent biological actions. Furthermore, new independent studies have demonstrated that IsoPs are the most reliable available marker of lipid peroxidation in vivo, and recent work examining IsoP formation has provided valuable infromation about the pathogenesis of numerous human diseases. Thus, the complexity of the IsoP pathway has expanded, providing novel insights into mechanisms of lipid peroxidation in vivo and allowing investigators to explore the role of oxidative stress in human disease.     

Compositional analysis and electrical properties of Sb,Mn-doped barium strontium titanate PTCR ceramics with TiO<Subscript>2</Subscript> and SiO<Subscript>2</Subscript> sintering additives

The secondary phase compositions in Sb-doped (Ba, Sr)TiO₃ ceramics containing SiO₂ and excess TiO₂ sintering additives have been examined by XRD, BEI and EPMA techniques. It is shown that alongside the primary (Ba_0.797Sr_0.2Sb_0.003)TiO₃ phase, (Ba_1.95Sr_0.05)₂(Ti_1.2Si_1.8)O₈ and Sb-doped (Ba_0.99Sr_0.01)₆Ti₁₇O₄₀ phases form at the intergranular regions. Substitution of up to 0.04 mol% MnO₂ enhances the PTCR effect, giving a ratio ρ_max/ρ_min ∼ 7 × 10⁵ for the optimum 0.04% MnO₂ composition. At this level Mn cannot be detected in the microstructure by EPMA, however in insulating samples containing 0.08 mol% of MnO₂, it was detected in the (Ba_0.99Sr_0.01)₆Ti₁₇O₄₀ intergranular phase.     

Spinal action of ketorolac, S(+)- and R(-)-ibuprofen on non-noxious activation of the catechol oxidation in the rat locus coeruleus: evidence for a central role of prostaglandins in the strychnine model of allodynia

BACKGROUND: Blockade of spinal glycine receptors with intrathecal strychnine produces an allodynia-like state in the anesthetized rat. Innocuous hair deflection in the presence of intrathecal strychnine induces a nociceptive-like activation of catechol oxidation in the locus coeruleus and enhances cardiovascular responses. Because prostaglandins play a central role in augmenting pain, this study evaluated the effect of intrathecal nonsteroidal antiinflammatory drugs in strychnine-induced allodynia. METHODS: In urethane-anesthetized rats, changes in catechol oxidation in the locus coeruleus, measured using in vivo voltammetry, and cardiovascular parameters evoked by hair deflection of caudal dermatomes were determined after strychnine (40 microg) or saline were administered intrathecally. Subsequently, the effects of 30 microg ketorolac, 10 microg S(+)-ibuprofen, and 10 microg R(-)-ibuprofen administered intrathecally were evaluated. RESULTS: After strychnine was administered intrathecally, hair deflection evoked an increase in the locus coeruleus catechol oxidation (peak, 149.7+/-7.2% of baseline) and mean arterial blood pressure (peak, 127.5+/-3.8% of baseline). These responses were not observed after saline was administered intrathecally. All hair deflection-evoked, strychnine-dependent peak responses were attenuated significantly with intrathecally administered ketorolac and S(+)-ibuprofen but not with R(-)-ibuprofen. CONCLUSIONS: Locus coeruleus catechol oxidation is a sensitive biochemical index of strychnine-induced allodynia and is correlated temporally with the cardiovascular responses evoked by hair deflection during spinal glycinergic inhibition. The ability of intrathecally administered ketorolac and S(+)-ibuprofen, but not R(-)-ibuprofen, to suppress the locus coeruleus catechol oxidation and cardiovascular peak responses evoked during strychnine-induced allodynia provide evidence that central prostaglandins play an important role in the abnormal sensory processing of strychnine-induced allodynia.