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111.
112.
Tarui Y Iida H Ono E Miki W Hirasawa E Fujita K Tanaka T Taniguchi M 《Journal of Bioscience and Bioengineering》2005,100(4):443-448
Transient expression of genes coding for the poly-gamma-glutamate (gammaPGA) synthetase system (pgs) was investigated in tobacco plants. Three genes of the pgs, pgsA, pgsB and pgsC, were separately placed under the control of the CaMV 35S promoter and introduced into tobacco leaves via Agrobacterium infection. Synthesized gammaPGA in plant tissues was detected immunologically with mouse anti-gammaPGA antiserum which specifically reacts with gammaPGA on a nitrocellulose membrane. Confirmation of gammaPGA biosynthesis in the transient expression analysis in tobacco tissue indicates that subunits of pgs complex were expressed and reassembled in a functional form. 相似文献
113.
Conjugated α-linolenic acid (ClnA) was prepared from α-linolenic acid (9,12,15–18∶3n−3, LnA) by alkaline treatment; we fractionated
CLnA into three peaks by reversed-phase column-HPLC as evidenced by monitoring absorbance at 205, 235, and 268 nm. Peak I
was a conjugated dienoic FA derived from LnA, whereas Peaks II and III were conjugated trienoic LnA. Proton NMR analysis showed
that Peak III consisted of the all-trans isomer. The methylated Peak III was further divided into five peaks (Peaks IV–VIII) by silver ion column-HPLC. Peak V, a
major constituent in the Peak III fraction, was identified as conjugated 10t,12t,14t-LnA by GC-EIMS and 1H NMR analysis. Peaks III and V, which consisted of conjugated all-trans trienoic LnA, had stronger growth-inhibitory effects on human tumor cell lines than the other collected peaks and strongly
induced lipid peroxidation as compared with Peaks I, II, and LnA. We propose that conjugated all-trans trienoic FA have the strongest growth-inhibitory effect among the conjugated trienoic acids and conjugated dienoic acids
produced by alkaline treatment of α-LnA, and that this effect is mediated by lipid peroxidation. 相似文献
114.
Sugimoto Shigetoki Minemura Kouichi Kobayashi Kohroh Seki Masafumi Shikada Minoru Ueki Atsufumi Yanase Tomoo Miki Tetsuya 《Electronics letters》1977,13(22):680-682
The letter reports results obtained with laboratory transmission experiments on high-speed digital signals by optical wavelength-division multiplexing, using a newly developed optical multiplexer and demultiplexer, as well as other high-performance optical devices and fibres. 相似文献
115.
Y Takemoto T Hata K Kamino N Mitsuda T Miki H Kawagoe T Ogihara 《Canadian Metallurgical Quarterly》1995,34(9):863-867
A 40-year-old female patient with Werner's syndrome (WS) suffering from thyroid cancer and myelodysplastic syndrome (MDS) is reported. She had been diagnosed as having WS complicated with thyroid cancer seven years previously. Total thyroidectomy and radioactive iodine (131I, 100 mCi/year) therapy for seven years had slowed the progression of thyroid cancer. She suffered a sudden onset of MDS at the age of 40 years. After six months she died from overt leukemia. We found an additional chromosome aberration of chromosome 10 in the progression of leukemia from MDS. 相似文献
116.
It is demonstrated through the use of transformation relations, function approximation and error limits that it is impossible to distinguish among rival gasification models using just a single temperature programmed reaction experiment. Two experiments at different heating rates must be used and the ratio between rates is important. These depend on the models to be distinguished. 相似文献
117.
118.
Toshikazu Takigawa Hidenori Kadoya Takashi Miki Takashi Yamamoto Toshiro Masuda 《Polymer》2006,47(13):4811-4815
Dynamic viscoelastic properties of a series of cyclic olefin copolymers have been investigated. The specimens differ in total molecular weight as well as molecular weight between entanglements. The angular frequency (ω) dependence curves of dynamic storage and loss moduli (G′ and G″, respectively) of the specimens have shown that G′ ∝ ω2 and G″ ∝ ω in the terminal region, and a plateau region at high ω. On the basis of the experimental results, the dependence of total molecular weight as well as molecular weight between entanglements has been examined for zero-shear viscosity (η0) and steady-state compliance (Je). It is shown that for the melts of the copolymers in the entangled regime, Mw being the weight-average molecular weight and Me the molecular weight between entanglements. The steady-state compliance Je for the melts scales with Me and Mw as . 相似文献
119.
Using a starter culture containing Lactococcus lactis subsp. cremoris and Acetobacter orientalis, repeated-batch culture for fermented-milk production was carried out for 14 d. The pH decreased to approximately 4.3 and the milk solidified stably when the mixing ratio of fermented-milk to fresh milk was set at a level as high as 10%. The microbial population gradually changed as the culture progressed, and the cell densities of lactic and acetic acid bacteria finally leveled off to constant values. The quality of the fermented-milk became almost constant with respect to the changes in the cell densities of the lactic and acetic acid bacteria. Escherichia coli was then inoculated into the fermented-milk to simulate household contamination. E. coli was washed out with the progress of the repeated-batch culture, and thus showed no adverse effects on the fermented-milk production. 相似文献
120.
Hiromasa Tanno Emi Kanno Suzuna Sato Yu Asao Mizuki Shimono Shiho Kurosaka Yukari Oikawa Shinyo Ishi Miki Shoji Ko Sato Jun Kasamatsu Tomomitsu Miyasaka Hideki Yamamoto Keiko Ishii Yoshimichi Imai Masahiro Tachi Kazuyoshi Kawakami 《International journal of molecular sciences》2021,22(8)
Chronic infections are considered one of the most severe problems in skin wounds, and bacteria are present in over 90% of chronic wounds. Pseudomonas aeruginosa is frequently isolated from chronic wounds and is thought to be a cause of delayed wound healing. Invariant natural killer T (iNKT) cells, unique lymphocytes with a potent regulatory ability in various inflammatory responses, accelerate the wound healing process. In the present study, we investigated the contribution of iNKT cells in the host defense against P. aeruginosa inoculation at the wound sites. We analyzed the re-epithelialization, bacterial load, accumulation of leukocytes, and production of cytokines and antimicrobial peptides. In iNKT cell–deficient (Jα18KO) mice, re-epithelialization was significantly decreased, and the number of live colonies was significantly increased, when compared with those in wild-type (WT) mice on day 7. IL-17A, and IL-22 production was significantly lower in Jα18KO mice than in WT mice on day 5. Furthermore, the administration of α-galactosylceramide (α-GalCer), a specific activator of iNKT cells, led to enhanced host protection, as shown by reduced bacterial load, and to increased production of IL-22, IL-23, and S100A9 compared that of with WT mice. These results suggest that iNKT cells promote P. aeruginosa clearance during skin wound healing. 相似文献