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991.
Correlation of In Vivo and In Vitro Assay Results for Assessment of Free Radical Scavenging Activity of Green Tea Nutraceuticals
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Heba‐Alla H. Abd‐ElSalam Medhat A. Al‐Ghobashy Muhammad Al‐Shorbagy Noha Nassar Hala E. Zaazaa Mohamed A. Ibrahim 《Journal of food science》2016,81(7):C1707-C1715
Green tea (GT)‐derived catechins; epigallocatechin gallate (EGCG) in particular are commonly used nutraceuticals for their free‐radical scavenging activity (FRSA). The influence of photodegradation on the protective power of GT nutracenticals against oxidative stress was thoroughly explored. Photodegradation of GT extracts was carried out and monitored using orthogonal stability‐indicating testing protocol; in vitro and in vivo assays. Total polyphenol content (TPC) and FRSA were determined spectrophotometrically while EGCG was selectively monitored using SPE‐HPLC. In vivo assessment of photodegraded samples was investigated via measuring a number of biomarkers for hepatic oxidative stress and apoptosis (caspase‐3, inducible nitric oxide synthase, nitric oxide, mitogen‐activated protein kinase, glutathione, thiobarbituric acid reactive substances, nuclear factor kappa beta, and nuclear factor erythroid 2‐related factor) as well as liver damage (alanine transaminase and aspartate transaminase) in serum of rats previously subjected to oxidative stress. Results showed complete degradation of EGCG in photodegraded green tea samples with no correlation with either TPC or FRSA. On the other hand, in vivo assay results revealed not only loss of activity but formation of harmful pro‐oxidants. Photostability was found crucial for the protective effect of GT extract against lead acetate insult. Results confirmed that careful design of quality control protocols requires correlation of chemical assays to bioassays to verify efficacy, stability, and most importantly safety of nutraceuticals. 相似文献
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Rashid Ahmad Ejaz Ahmad Waraich Fahim Nawaz Muhammad Y Ashraf Muhammad Khalid 《Journal of the science of food and agriculture》2016,96(2):372-380
Climate change has emerged as one of the most complex challenges of the 21st century and has become an area of interest in the past few decades. Many countries of the world have become extremely vulnerable to the impacts of climate change. The scarcity of water is a serious concern for food security of these countries and climate change has aggravated the risks of extreme events like drought. Oxidative stress, caused by a variety of active oxygen species formed under drought stress, damages many cellular constituents, such as carbohydrates, lipids, nucleic acids and proteins, which ultimately reduces plant growth, respiration and photosynthesis. Se has become an element of interest to many biologists owing to its physiological and toxicological importance. It plays a beneficial role in plants by enhancing growth, reducing damage caused by oxidative stress, enhancing chlorophyll content under light stress, stimulating senesce to produce antioxidants and improving plant tolerance to drought stress by regulating water status. Researchers have adopted different strategies to evaluate the role of selenium in plants under drought stress. Some of the relevant work available regarding the role of Se in alleviating adverse effect of drought stress is discussed in this paper. © 2015 Society of Chemical Industry 相似文献
993.
Mizher Hezam AL‐Zuaidy Azizah Abdul Hamid Amin Ismail Suhaila Mohamed Ahmad Faizal Abdul Razis Muhammad Waseem Mumtaz Syafiq Zikri Salleh 《Journal of food science》2016,81(5):C1080-C1090
Diabetes mellitus is normally characterized by chronic hyperglycemia associated with disturbances in the fat, carbohydrate, and protein metabolism. There is an increasing trend of using natural products instead of synthetic agents as alternative therapy for disorders due to their fewer side effects. In this study, antidiabetic and antioxidant activities of different Melicope lunu‐ankenda (ML) ethanolic extracts were evaluated using inhibition of α‐glucosidase and 2,2‐diphenyl‐l‐picrylhydrazyl (DPPH) radicals scavenging activity, respectively; whereas, proton nuclear magnetic resonance (1H NMR) and ultra‐high performance liquid chromatography‐tandem mass spectrometric (UHPLC‐MS/MS) techniques were used for metabolite profiling of ML leaf extracts at different concentrations of ethanol and water. Sixty percent of ethanolic ML extract showed highest inhibitory effect against α‐glucosidase enzyme (IC50 of 37 μg/mL) and DPPH scavenging activity (IC50 of 48 μg/mL). Antidiabetic effect of ML extracts was also evaluated in vivo and it was found that the high doses (400 mg/Kg BW) of ML extract exhibited high suppression in fasting blood glucose level by 62.75%. The metabolites responsible for variation among ML samples with variable ethanolic levels have been evaluated successfully using 1H‐NMR–based metabolomics. The principal component analysis (PCA) and partial least squares(PLS) analysis scores depicted clear and distinct separations into 4 clusters representing the 4 ethanolic concentrations by PC1 and PC2, with an eigenvalue of 69.9%. Various 1H‐NMR chemical shifts related to the metabolites responsible for sample difference were also ascribed. The main bioactive compounds identified attributing toward the separation included: isorhamnetin, skimmianine, scopoletin, and melicarpinone. Hence, ML may be used as promising medicinal plant for the development of new functional foods, new generation antidiabetic drugs, as a single entity phytomedicine or in combinational therapy. 相似文献
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为了深入研究中巴喀喇昆仑公路洪扎河以北地段的顺层岩质边坡弯曲失稳机制,采用材料力学压杆稳定及刚性体平衡原理,在D.S.Cavers的基础上考虑地震力作用,分析得到岩质边坡的三种弯曲模型(平面边坡的柔性弯曲、平面边坡的三铰梁模型、曲线边坡的三绞铰模型)的失稳公式,将地震与非地震作用下平面边坡的柔性弯曲进行对比分析。研究结果表明:当内摩擦角φj处于0°~15°范围时,若水平地震加速度方向与岩体弯曲方向相反,平面边坡柔性弯曲模型在地震作用下不易发生弯曲变形;若水平地震加速度方向与岩体弯曲方向一致,地震作用下平面边坡柔性弯曲模型的临界状态总长度l总比非地震作用下的l小,平面边坡在此方向的地震作用下更易发生柔性弯曲变形。 相似文献
999.
Muhammad Saqib Shahid Iqbal Asif Mahmood Raheel Akram 《International Journal of Food Properties》2016,19(4):745-751
The main objective of this research was to conduct a theoretical investigation into the antioxidant properties of chlorogenic acid. Calculations based on the density functional theory were performed using the B3LYP exchange-correlation functional and the 6-311G standard basis set to determine the theoretical parameters, such as bond dissociation enthalpy and spin density. H atom abstraction from ?OH groups was the most representative reaction pathway chosen for this study. From these calculations, it was estimated that bond dissociation enthalpies of hydroxyl groups were in the order of 3’-OH > 4’-OH > 2-OH > 1-OH. Spin densities of O-atoms of free radicals formed (after the removal of H atoms) from the hydroxyl groups of aromatic rings were lower than that of hydroxyl groups of alicyclic rings. On the basis of the calculated results, H atom abstraction from ?OH groups of aromatic ring should be the main mechanism for the antioxidant potential of chlorogenic acid. Moreover, a comparison of bond dissociation enthalpy values for different phenolic acid antioxidants showed that the chlorogenic acid is a potent antioxidant. This theoretical investigation might be helpful for the future applications of chlorogenic acid in the field of the pharmacy and food industries. 相似文献
1000.
Syed M. Nasar‐Abbas Zill‐ e‐Huma Thi‐Huong Vu Muhammad Kamran Khan Henry Esbenshade Vijay Jayasena 《Comprehensive Reviews in Food Science and Food Safety》2016,15(1):63-72
Carob (Ceratonia siliqua L.) is well known for its valuable locust bean gum obtained from the carob seeds. Separation of seeds from the pod leaves behind the carob kibble which is a good source of dietary fiber, sugars, and a range of bioactive compounds such as polyphenols and pinitol. Bioactive compounds present in carob kibble have been found to be beneficial in the control of many health problems such as diabetes, heart diseases, and colon cancer due to their antidiabetic, antioxidant, and anti‐inflammatory activities. Carob kibble has substantial potential to be used as a food ingredient. This article focuses on the composition, health benefits, and food applications of carob kibble. 相似文献