Influence of Bone Substitutes on Mesenchymal Stromal Cells in an Inflammatory Microenvironment

Bone regeneration is driven by mesenchymal stromal cells (MSCs) via their interactions with immune cells, such as macrophages (MPs). Bone substitutes, e.g., bi-calcium phosphates (BCPs), are commonly used to treat bone defects. However, little research has focused on MSC responses to BCPs in the context of inflammation. The objective of this study was to investigate whether BCPs influence MSC responses and MSC–MP interactions, at the gene and protein levels, in an inflammatory microenvironment. In setup A, human bone marrow MSCs combined with two different BCP granules (BCP 60/40 or BCP 20/80) were cultured with or without cytokine stimulation (IL1β + TNFα) to mimic acute inflammation. In setup B, U937 cell-line-derived MPs were introduced via transwell cocultures to setup A. Monolayer MSCs with and without cytokine stimulation served as controls. After 72 h, the expressions of genes related to osteogenesis, healing, inflammation and remodeling were assessed in the MSCs via quantitative polymerase chain reactions. Additionally, MSC-secreted cytokines related to healing, inflammation and chemotaxis were assessed via multiplex immunoassays. Overall, the results indicate that, under both inflammatory and non-inflammatory conditions, the BCP granules significantly regulated the MSC gene expressions towards a pro-healing genotype but had relatively little effect on the MSC secretory profiles. In the presence of the MPs (coculture), the BCPs positively regulated both the gene expression and cytokine secretion of the MSCs. Overall, similar trends in MSC responses were observed with BCP 60/40 and BCP 20/80. In summary, within the limits of in vitro models, these findings suggest that the presence of BCP granules at a surgical site may not necessarily have a detrimental effect on MSC-mediated wound healing, even in the event of inflammation.     

Free vibration analysis of multi-symmetric stiffened shells

The application of structural symmetry techniques to the free vibration analysis of cylindrical and conical shells for the prediction of natural frequencies and mode shapes is described. Appropriate boundary conditions have been developed for the analysis of only a part of the shell and have been shown to yield results comparable to the full shell analysis. Half and quarter models of the shell have been developed and analysed using semi-loof and facet shell finite elements. Unstiffened and stiffened circular cylindrical shells and stiffened conical shells have been considered.     

Assured learning-enabled autonomy: A metacognitive reinforcement learning framework

Reinforcement learning (RL) agents with pre-specified reward functions cannot provide guaranteed safety across variety of circumstances that an uncertain system might encounter. To guarantee performance while assuring satisfaction of safety constraints across variety of circumstances, an assured autonomous control framework is presented in this article by empowering RL algorithms with metacognitive learning capabilities. More specifically, adapting the reward function parameters of the RL agent is performed in a metacognitive decision-making layer to assure the feasibility of RL agent. That is, to assure that the learned policy by the RL agent satisfies safety constraints specified by signal temporal logic while achieving as much performance as possible. The metacognitive layer monitors any possible future safety violation under the actions of the RL agent and employs a higher-layer Bayesian RL algorithm to proactively adapt the reward function for the lower-layer RL agent. To minimize the higher-layer Bayesian RL intervention, a fitness function is leveraged by the metacognitive layer as a metric to evaluate success of the lower-layer RL agent in satisfaction of safety and liveness specifications, and the higher-layer Bayesian RL intervenes only if there is a risk of lower-layer RL failure. Finally, a simulation example is provided to validate the effectiveness of the proposed approach.     

The modification of the characteristics of ZnO nanofibers by TCNQ doping content

In this study, the electrical properties of an Al/p-Si metal/semiconductor photodiodes with Tetracyanoquinodimethane–Polyvinyl chloride (TCNQ–PVC) and PVC–TCNQ:ZnO interfacial layers were investigated. Growing of the interfacial layers on p-Si were fulfilled using electrospinning method as a fiber form. Al metallic and ohmic contacts were deposited via physical vapor deposition method. Scanning electron microscopy (SEM) pictures of the devices were captured to examine the morphology of the structure. Within the scope of electrical characterization, I–V measurements of the Al/PVC–TCNQ/p-Si and Al/PVC–TCNQ:ZnO/p-Si devices were accomplished both in the dark and under illumination conditions. Various device parameters, such as ideality factor and barrier height values were determined from I–V characteristics. Although the ideality factor values were obtained as 8.47 and 6.85 for undoped and ZnO-doped Al/PVC–TCNQ/p-Si diodes, the barrier height values were calculated as 0.84 for both devices. When a comparison was made between ZnO doped and undoped Al/PVC–TCNQ/p-Si diodes, it was evaluated that the rectification and photoresponse properties of the heterojunction diode was improved with ZnO dopant.

     

Coronary artery bypass grafting versus percutaneous coronary intervention in end-stage kidney disease: A systematic review and meta-analysis of clinical studies

The most significant complication of end-stage kidney disease (ESKD) is cardiovascular disease, mainly coronary artery disease (CAD). Although the effective treatment of CAD is an important prognostic factor, whether percutaneous coronary intervention (PCI) or coronary artery bypass grafting (CABG) is better for treating CAD in this group of patients is still controversial. We searched Pubmed/Medline, Web of Science, Embase, the Cochrane Central Register of Controlled Trials articles that compared the outcomes of CABG versus PCI in patients with ESKD requiring dialysis. A total of 10 observational studies with 39,666 patients were included. Our analysis showed that when compared to PCI, CABG had lower risk of need for repeat revascularization (relative risk [RR] = 2.25, 95% confidence interval [CI] 2.1–2.42, p < 0.00001) and cardiovascular death (RR = 1.19, 95% CI 1.14–1.23, p < 0.00001) and higher risk for short-term mortality (RR = 0.43, 95% CI 0.38–0.48, p < 0.00001). There was no statistically significant difference between the PCI and CABG groups in the risk for late mortality (RR = 1.05, 95% CI 0.97–1.14, p = 0.25), myocardial infarction (RR = 1.05, 95% CI 0.46–2.36, p = 0.91) or stroke (RR = 1.02, 95% CI 0.64–1.61, p = 0.95). This meta-analysis showed that in ESKD patients requiring dialysis, CABG was superior to PCI in regard to cardiovascular death and need for repeat revascularization and inferior to PCI in regard to short term mortality. However, this meta-analysis has limitations and needs confirmation with large randomized controlled trials.     

Vertical Handover Decision Algorithm for Multimedia Streaming in VANET

Wireless Personal Communications - Vehicular ad hoc networks represent one of the most important applications of wireless ad hoc networks. In highways, where different networks exist, frequent and...     

Optical solitons to the nonlinear Shrödinger’s equation with spatio-temporal dispersion using complex amplitude ansatz

In this work, we investigate the optical solitons to the non-linear Shrödinger’s equation with spatio-temporal dispersion. There are two types of non-linear media studied in this paper. They are Kerr law and parabolic law. By adopting a complex amplitude ansatz method composed of the addition of bright and dark optical solitons, we present the exact dark, bright and dark-bright or combined optical solitons to the model. Numerical results and discussions are also presented.     

Designing,modeling and performance investigation of bio-inspired flow field based DMFCs

In an attempt to improve upon conventional flow fields (e.g., serpentine flow field), Murray's Law was applied to design two different bio-inspired, leaf-shaped flow fields. This law governs the dimensions of natural networks, such as: the veins within plant leaves and human lungs. In this study, the serpentine, the lung, and the two leaf-shaped flow fields were used to form seven different anode–cathode combinations. The experiments focused on the effects of methanol concentration (0.50 M, 0.75 M, and 1.00 M) and the combined effect of methanol and oxygen flow rates (1.3 ml/min methanol and 400 ml/min oxygen, as well as 2 and 3 times both of these flow rates). An analytical model was also developed to help understand the experimental results. The results show that the highest performance could be achieved when the bio-inspired configurations were used on the cathode. The best configuration was the serpentine (anode) – second leaf design (cathode), with a peak power density of 888 W/m². For comparison, a peak power density of 824 W/m² was achieved when the serpentine flow field was used on the anode and cathode. Furthermore, of all the tested configurations, the lung-based flow field provided the lowest performance in all tests.