Bidirectional transport of cadmium across apical membrane of renal epithelial cell lines via H+-antiporter and inorganic anion exchanger

Faecal swabs obtained from a random sample of 268 cows and 90 calves on 19 Lugo (northwestern Spain) farms were examined for necrotoxigenic Escherichia coli (NTEC) producing the cytotoxic necrotizing factors type 1 (CNF1) and type 2 (CNF2). We found NTEC CNF1+ and CNF2+ on 11% and 95% of the farms, respectively, NTEC producing CNF2 were significantly more frequently isolated from calves (58%) than from cows (17%) (P < 0.001). The proportion of animals colonized with CNF2+ strains on each farm ranged from 0% to 60%. NTEC strains producing CNF2 isolated from healthy cattle belonged to 27 O serogroups; however, 64% were of one of 12 serogroups (O2, O8, O8-O75, O14, O15, O55, O86, O88, O115, O121, O147, and O168). Furthermore, the serogroups determined in CNF2+ strains isolated from cows (O2, O8, and O14) were different from those found in NTEC producing CNF2 isolated from calves (O8-O75, O15, O55, O86, O88, O115 and O147).     

Hepatitis C virus infection and heart diseases: a multicenter study in Japan

We present our experience of transcutaneous truncal anaesthesia of the maxillary nerve in association with transmucosal anaesthesia of the sphenopalatine ganglion in surgically assisted rapid maxillary expansion. Twelve patients with a skeletal transverse discrepancy of the maxilla were treated in our department from 1994 to 1995. Maxillary transcutaneous nerve block was done with a Quincke 8 cm spinal needle together with transmucosal anaesthesia of the sphenopalatine ganglion. Mepivacaine without adrenaline and sodium bicarbonate 1/10 was used for truncal anaesthesia and lidocaine-prilocaine cream for transmucosal anaesthesia. A Le Fort I osteotomy, lateral nasal wall osteotomy, pterygomaxillary osteotomy, and a palatal osteotomy were done for all patients before the maxillary expansion. Total anaesthesia of the maxillary area facilitated the operations and appreciably reduced the amount of postoperative pain. The ease of achieving effective anaesthesia before and after operation and the absence of side-effects make this form of anaesthetic particularly useful in surgically assisted rapid maxillary expansion.     

Alleviation of monocrotaline-induced pulmonary hypertension by antibodies to monocyte chemotactic and activating factor/monocyte chemoattractant protein-1

Administration of monocrotaline (MCT) causes pulmonary vascular lesions consisting of monocyte/macrophage infiltration in the early phase and medial thickening in pulmonary arteries and arterioles associated with pulmonary hypertension (PH) in the later phase. However, the molecular mechanism of monocyte/macrophage infiltration and its roles remain elusive. Herein, we have evaluated the role of a potent monocyte chemotactic and activating chemokine/monocyte chemoattractant protein-1 (MCAF/MCP-1) in MCT-induced PH in rats. A single injection of MCT induced PH at Day 21, as evidenced by increases in the ratio of right ventricular to left ventricular and septum weights (RV/LV+S) and right ventricular systolic pressure (RVSP). A significant increase in macrophage number in lungs started at Day 14, reaching a maximum at Day 21. MCAF/MCP-1 levels in bronchoalveolar lavage fluids were elevated significantly at Day 14 and remained high until Day 28, whereas plasma MCAF/MCP-1 levels increased at Day 7, returning to normal levels by Day 21. Immunoreactive MCAF/MCP-1 proteins were mainly detected in macrophages in alveoli and in perivascular regions of pulmonary arterioles and venules. Intravenous administration of anti-MCAF/MCP-1 antibodies with MCT significantly decreased macrophage infiltration and eventually reduced the increases in RV/LV+S and RVSP, as well as medial thickening of pulmonary arterioles. Thus, MCAF/MCP-1 is essentially involved in MCT-induced PH by recruiting and activating macrophages.     

Online Earthquake Response Test for Stratified Layers of Clay and Sand

Artificial islands often consist of layers of alluvial clay and reclaimed soil of varying order and thickness. Soft clay layers have nonlinear characteristics and can both amplify and attenuate earthquake ground motions. Liquefied ground impedes propagation of shear waves and thus attenuates the earthquake accelerations. Online testing is a method of feeding soil response characteristics directly from soil samples into a modeling algorithm. The effects of the layer thickness, configuration, and degree of consolidation on the earthquake response characteristics of alternating layers of clay and sand have been investigated. The degree of liquefaction and strain generated in sand adjacent to clay layers increased with the degree of consolidation. Clay layers attenuate the motions of sand layers for short period vibrations but amplify the long period motions, increasing the strain in overlying liquefied sand layers. Clay layers which were closer to the ground surface or of greater thickness tended to increase the surface accelerations. Normalized cumulative energy loss was larger in clay than in sand increasing with a decreasing degree of consolidation.     

Tensile-strained multiple quantum-well structures for a largerefractive index change caused by current injection

Tensile-strained multiple quantum-well (MQW) structures with camel-back shaped first valence sub-bands are proposed as structures with a large refractive index change caused by current injection. These structures have a high joint density of states at the absorption edge, and the injected carriers in the structures have a long lifetime because of separation in the k-space between electrons and holes. The refractive index change caused by current injection is calculated for camel-back InGaAs/InGaAsP strained MQW structures for 1.55 μm-wavelength light. These structures show a larger refractive index change than the other InGaAs/InGaAsP strained/unstrained MQW structures     

A 3.84 GIPS integrated memory array processor with 64 processingelements and a 2-Mb SRAM

An integrated memory array processor (IMAP) ULSI with 64 processing elements and a 2-Mb SRAM has been developed for image processing. The chip attains a 3.84 GIPS peak performance through the use of SIMD parallel processing and a 1.28 GByte/s on-chip processor-memory bandwidth. The IMAP is capable of parallel indirect addressing, which increases applications for parallel algorithms. Large power consumption with the wide memory bandwidth is avoided by reducing the number of active sense amplifiers and adopting dynamic power control. Fabricated with a 0.55-μm BiCMOS double layer metal process technology, the IMAP contains 11 million transistors in a 15.1×15.6 mm² die area     

A design technique for a 60 GHz-bandwidth distributed basebandamplifier IC module

A DC-60 GHz, 9 dB distributed amplifier IC module is fabricated with 0.15 μm InAlAs-InGaAs low-noise HEMTs with 155 GHz f_T and 234 GHz f_max. The device is mounted in a metal package with 1.8 mm coaxial cable signal interfaces. The package is specially designed using three-dimensional electromagnetic field analyses, resulting in very flat frequency characteristics of the module within 1.5 dB gain ripples over the entire bandwidth. A multichip module loaded with two amplifier ICs in cascade is also fabricated, and operates at a 17.5 dB gain from 60 kHz to 48 GHz. The 1 dB gain compression output power is about 5 dBm for both modules. The noise figure of the single-chip module is approximately 4 dB over a 10-40 GHz frequency range     

A compact programming environment for microprocessor-based controllers

To write a reliable program in an assembly language for microprocessor-based controller, we introduce data abstraction facility into an assembly language. For this purpose, we has designed and implemented ADT-RASM86 and its symbolic debugger. This paper presents the figure of the system and describes how the system is effective for writing a reliable program for microprocessors.     

Compositional range,thermal stability,hardness and electrical resistivity of amorphous alloys in Al-Si (or Ge)-transition metal systems

An amorphous single phase was found to be formed in wide compositional ranges in rapidly solidified Al-Si-transition metal (M) and Al-Ge-M alloys. The compositional ranges are in the range from 12 to 53 at. % Si or Ge and 8 to 23% M and Al-Si-Co and Al-Ge-Fe alloys have the widest glass-formation ranges. Because the interaction between aluminium and silicon or germanium atoms is thought to be repulsive from the immiscible equilibrium phase diagrams, the glass formation is probably due to an attractive interaction of M-Si (or Ge) and Al-M pairs. Hardness, H _v, and crystallization temperature, T _x, increase with increasing M content and the highest values reach 1120 DPN and 715 K, while the change with silicon or germanium content is much smaller for H _v and is hardly seen for T _x. Additionally, the H _v and T _x have maximum values for Al-Si (or Ge)-M (M=Cr, Mn or Fe), decrease with the decrease and increase in the group number of M element and are the lowest for Al-Si (or Ge)-Ni alloys. The compositional dependence is interpreted under the assumption that T _x and H _v of the aluminium-based amorphous alloys are mainly dominated by the attractive interaction of M-(Si or Ge) and Al-M pairs. Room-temperature resistivity, _RT, increases in the range of 220 to 1940 cm with increasing silicon or germanium and M contents. The change in _RT with the group number of M elements shows a maximum phenomenon for manganese. It has thus been clarified that the characteristics of the Al-Si-M and Al-Ge-M amorphous alloys have the different compositional dependence as compared with those for conventional metalmetalloid amorphous alloys, probably because of the unusual interaction among the constituent elements.     

A brush device with visual and haptic feedback for virtual painting of 3D virtual objects

We have previously developed a mixed reality (MR) painting system with which a user could take a physical object in the real world and apply virtual paint to it. However, this system could not provide the sensation of painting on virtual objects in MR space. Therefore, we subsequently proposed and developed mechanisms that simulated the effect of touch and movement when a brush device was used to paint on a virtual canvas. In this paper, we use visual and haptic feedback to provide the sensation of painting on virtual three-dimensional objects using a new brush device called the MAI Painting Brush++. We evaluate and confirm its effectiveness through several user studies.