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31.
Joanna CabajAuthor VitaeJadwiga So?oduchoAuthor Vitae Antoni ChylaAuthor VitaeAgnieszka J?drychowska 《Sensors and actuators. B, Chemical》2011,157(1):225-231
Electrolytic deposition has been widely used to immobilize biomacromolecules, and it is always the most important factor to preserve or even increase an activity of the immobilized protein. We report here simple and rather universal method for the highly efficient immobilization of laccase for amperometric biosensing. Laccase from Cerrena unicolor has been successfully immobilized (electrolytic deposition) on the surface of thin, ordered polythiophene films (3-methylthiophene/3-thiopheneacetic acid/N-heptyl-3,6-bis(2-thiophene)carbazole). Two different compounds capable of mediating laccase-catalyzed reactions have been tested by cyclic voltammetry. They exhibited quasi-reversible electrodic behaviour with formal redox potentials ranging from 68 and 918 mV (E0vs. SCE). The immersion of the laccase-coated electrode in solution with substrate generated large catalytic currents easily recorded by cyclic voltammetry at low potential scan rates. Considering the fact, that immobilization strategy showed high efficiency, obtained results suggest that method for phenoloxidase immobilization has a great potential of enabling high throughput fabrication of bioelectronics’ devices. 相似文献
32.
The notions of $(\overline{\in}, \overline{\in} \vee \overline{\hbox{q}})The notions of ([`( ? )],[`( ? )] ú[`q])(\overline{\in}, \overline{\in} \vee \overline{\hbox{q}})-fuzzy p-ideals and fuzzy p-ideals with thresholds related to soft set theory are discussed. Relations between ([`( ? )],[`( ? )] ú[`q])(\overline{\in}, \overline{\in} \vee \overline{\hbox{q}})-fuzzy ideals and ([`( ? )],[`( ? )] ú[`q])(\overline{\in}, \overline{\in} \vee \overline{\hbox{q}})-fuzzy p-ideals are investigated. Characterizations of an ([`( ? )],[`( ? )] ú[`q])(\overline{\in}, \overline{\in} \vee \overline{\hbox{q}})-fuzzy p-ideal and a fuzzy p-ideal with thresholds are displayed. Implication-based fuzzy p-ideals are discussed. 相似文献
33.
We consider a problem of dynamic stochastic portfolio optimization modelled by a fully non-linear Hamilton–Jacobi–Bellman (HJB) equation. Using the Riccati transformation, the HJB equation is transformed to a simpler quasi-linear partial differential equation. An auxiliary quadratic programming problem is obtained, which involves a vector of expected asset returns and a covariance matrix of the returns as input parameters. Since this problem can be sensitive to the input data, we modify the problem from fixed input parameters to worst-case optimization over convex or discrete uncertainty sets both for asset mean returns and their covariance matrix. Qualitative as well as quantitative properties of the value function are analysed along with providing illustrative numerical examples. We show application to robust portfolio optimization for the German DAX30 Index. 相似文献
34.
35.
N. K. S. Lee R. S. Goonetilleke Y. S. Cheung Geommi M. Y. So 《Microsystem Technologies》2001,7(2):55-62
The use of pressure sensors made of conductive polymers is common in biomechanical applications. Unfortunately, hysteresis,
nonlinearity, non-repeatability and creep have a significant effect on the pressure readings when such conductive polymers
are used. The objective of this paper is to explore the potential of a new flexible encapsulated micro electromechanical system
(MEMS) pressure sensor system as an alternative for human interface pressure measurement. A prototype has been designed, fabricated,
and characterized. Testing has shown that the proposed packaging approach shows very little degradation in the performance
characteristics of the original MEMS pressure sensor. The much-needed characteristics of repeatability, linearity, low hysteresis,
temperature independency are preserved. Thus the flexible encapsulated MEMS pressure sensor system is very promising and shows
superiority over the commercially available conductive polymer film sensors for pressure measurement in biomechanical applications.
Received: 1 December 1999/Accepted: 17 August 2000 相似文献
36.
This paper reports on the types and magnitudes of localization errors of simulated binaural direction cues generated using non-individualized, head-related transfer functions (HRTFs) with different levels of complexity. Four levels of complexity, as represented by the number of non-zero coefficients of the associated HRTF filters (128, 64, 32, 18 non-zero coefficients), were studied. Experiment 1 collected 1728 data runs that were exhaustive combinations of the four levels of complexity, nine simulated directions of sound (no direction (i.e., diotical-mono), 0 degrees , 45 degrees , 90 degrees , 135 degrees , 180 degrees , 225 degrees , 270 degrees , and 315 degrees azimuth angles at 0 degrees elevation), two repetitions, and 24 participants). Binaural cues generated from HRTFs of reduced complexity (from 128 to 18 non-zero coefficients) produced significantly higher localization errors for the directions of 45 degrees , 135 degrees , 225 degrees , and 315 degrees azimuth angles (p<0.01). From the directions of 0 degrees , 90 degrees , and 270 degrees azimuth angles, the cues produced by HRTFs with reduced complexity did not affect the localization error (p>0.2). Surprisingly, cues produced by HRTFs of 128 non-zero coefficients did not have the lowest number of errors. From 45 degrees , 135 degrees , 225 degrees , and 315 degrees , the lowest numbers of errors were obtained from cues produced by HRTFs of 64, 32, 32, and 64 non-zero coefficients, respectively. Based on these findings, a prototype virtual headphone-based surround-sound (VHSS) system was developed. A double-blind usability experiment with 32 participants indicated that the prototype VHSS system received significantly better surround-sound ratings than did a Dolby stereo system (p<0.02). This paper reports results from an original ergonomics study and the application of these results to the design of a consumer product. 相似文献
37.
The sensitivity of a neural network's output to its input and weight perturbations is an important measure for evaluating the network's performance. In this letter, we propose an approach to quantify the sensitivity of Madalines. The sensitivity is defined as the probability of output deviation due to input and weight perturbations with respect to overall input patterns. Based on the structural characteristics of Madalines, a bottom-up strategy is followed, along which the sensitivity of single neurons, that is, Adalines, is considered first and then the sensitivity of the entire Madaline network. By means of probability theory, an analytical formula is derived for the calculation of Adalines' sensitivity, and an algorithm is designed for the computation of Madalines' sensitivity. Computer simulations are run to verify the effectiveness of the formula and algorithm. The simulation results are in good agreement with the theoretical results. 相似文献
38.
Modeling and measurement of simultaneous switching noise coupling through signal via transition 总被引:1,自引:0,他引:1
Jongbae Park Hyungsoo Kim Youchul Jeong Jingook Kim Jun So Pak Dong Gun Kam Joungho Kim 《Advanced Packaging, IEEE Transactions on》2006,29(3):548-559
The signal via is a heavily utilized interconnection structure in high-density System-on-Package (SoP) substrates and printed circuit boards (PCBs). Vias facilitate complicated routings in these multilayer structures. Significant simultaneous switching noise (SSN) coupling occurs through the signal via transition when the signal via suffers return current interruption caused by reference plane exchange. The coupled SSN decreases noise and timing margins of digital and analog circuits, resulting in reduction of achievable jitter performance, bit error ratio (BER), and system reliability. We introduce a modeling method to estimate SSN coupling based on a balanced transmission line matrix (TLM) method. The proposed modeling method is successfully verified by a series of time-domain and frequency-domain measurements of several via transition structures. First, it is clearly verified that SSN coupling causes considerable clock waveform distortion, increases jitter and noise, and reduces margins in pseudorandom bit sequence (PRBS) eye patterns. We also note that the major frequency spectrum component of the coupled noise is one of the plane pair resonance frequencies in the PCB power/ground pair. Furthermore, we demonstrate that the amount of SSN noise coupling is strongly dependent not only on the position of the signal via, but also on the layer configuration of the multilayer PCB. Finally, we have successfully proposed and confirmed a design methodology to minimize the SSN coupling based on an optimal via positioning approach. 相似文献
39.
Ying-Hung So Stark E. J. Yongfu Li Kisting S. Achen A. Baranek K. Scheck D. Hetzner J. Folkenroth J. J. Tpper M. Baumgartner T. 《Advanced Packaging, IEEE Transactions on》2006,29(4):741-750
A positive-tone and aqueous-base-developable benzocyclobutene (BCB)-based dielectric material curable in air is described in this paper. The prepolymer is made from divinylsiloxane bisbenzocyclobutene (DVS-bisBCB) and BCB-acrylic acid. The formulation contains antioxidants that allow the prepolymer to cure in air and a diazonaphthaquinone to make it photosensitive. Patterned films have high resolution, and via openings are scum-free without a descum operation. Whether cured in nitrogen or in air, the prepolymer produces a film with optical, electrical, thermal, and mechanical properties desirable for many microelectronic applications, such as packaging applications and as a planarization or insulation layer in display applications 相似文献
40.
In this note, the problem of the frequency estimation of a sinusoid embedded in white noise is considered. The approach used herein is the minimization of the sample variance of the output of constrained notch filters fed by the noisy sinusoid. In particular, this note focuses on closed-form expressions of the frequency estimate, which can be obtained using notch filters having an all-zeros finite-impulse response (FIR) structure. The results presented in this note are as follows: 1) it is shown that the FIR notch filters obtained from standard second-order infinite-impulse response (IIR) filters are inadequate; 2) a new second-order IIR notch filter is proposed, which provides an unbiased estimate of the frequency; 3) the FIR filter obtained from the new IIR filter provides a closed-form unbiased frequency estimate; and 4) the closed-form frequency estimate obtained using the new FIR notch filter asymptotically converges toward the Pisarenko harmonic decomposition estimator and the Yule-Walker estimator. 相似文献